Formation of Biocompatible Nanoparticles by Self-Assembly of Enzymatic Hydrolysates of Chitosan and Carboxymethyl Cellulose

Ichikawa, Sosaku; Iwamoto, Satoshi; Watanabe, Jun

doi:10.1271/bbb.69.1637

Cited by 65 publications

(33 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results are in line with preceding studies on the swelling of CMC-based nanoparticles at lower pH values, induced by loosened electrostatic linkages of CMC and Ca 2 + due to the decrease in negative charge density on the polymer. [39] As expected, the increase in the CaCl 2 /CMC ratio results in an increase in the particle size (Figure 4 c). A CaCl 2 /CMC ratio of over 0.5 with a {CoPW 9 O 34 }/CMC ratio of 0.2 at pH 7 leads to a fast aggregation of the particles.…”

Section: Resultssupporting

confidence: 80%

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Geisberger

Paulus

Carraro

et al. 2011

Chemistry A European J

View full text Add to dashboard Cite

Chitosan and its derivates continue to attract considerable research interest as effective drug carriers with good biocompatibility and high cellular uptake rates. We used these versatile features to tap the considerable biomedical potential of polyoxometalates (POMs) through their encapsulation into a carboxymethyl chitosan (CMC) matrix. The nanocapsules were prepared by ionic gelification with Ca(2+); their size distribution ranges from 60 to 150 nm. Because [Co(4)(H(2)O)(2)(PW(9)O(34))(2)](10-) is well known for its manifold properties, such as antiviral activity, it was selected as a model POM. The resulting composites were characterised with a wide range of analytical methods, which pointed to quantitative encapsulation of intact POMs within the CMC matrix. We studied the biocompatibility of the POM/CMC nanocomposites on HeLa cells through MTT and proliferation assays. Even after prolonged incubation times at high concentrations, the composites did not display cytotoxicity, thereby drastically reducing the side effects of the pristine POMs. This opens up new avenues for designing novel inorganic drug prototypes from bioactive POMs.

show abstract

Section: Resultssupporting

confidence: 80%

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Geisberger

Paulus

Carraro

et al. 2011

Chemistry A European J

View full text Add to dashboard Cite

show abstract

“…Chitosan is accompanied by only one polyanionic substance, such as tripolyphosphate (TPP) (1e9), alginate (10,11), dextran sulfate (12), cellulose-hydrolyzed carboxymethyl cellulose (13), heparin (14,15), DNA (16), glycyrrhetic acid (17), carboxymethyl konjac glucomannan and poly-gamma glutamic acid (18,19), and fucoidan (20e22). Nanoparticles have distinct advantages over microparticles, such as a relatively higher intracellular uptake.…”

mentioning

confidence: 99%

Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis

Lee

Lim

2016

Journal of Bioscience and Bioengineering

View full text Add to dashboard Cite

“…Electrostatic complexation can be used to formulate biopolymer particles. Depending on the biopolymers used, either negatively or positively charged biopolymer particles can be formed (16). The advantage of a biopolymer based-system is that the materials are from natural resources using preparation methods.…”

Section: Nano-science-engineering-technology Applications Tomentioning

confidence: 99%

Nano-Science-Engineering-Technology Applications to Food and Nutrition

Nakajima

Wang

Chaudhry

et al. 2015

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

The potential benefits of nanotechnology have been recognized by many industrial sectors, and nanotechnology products are increasingly appearing in the world as consumer products. It has also wide application in the field of food: nano-sensors, smart packaging, nanoencapsulation and delivery of food compounds. Nanotechnology can be applied to the formulation of novel materials at nanoscale, as well as the development of new techniques for research to provide innovative systems. Nanotechnology is expected to open new opportunities in the field of science and technology.In the conference, the following 4 topics will be presented. Nakajima will present "Micro/Nano-Engineering for Formulation of Functional Foods". Health benefits related to functional lipids have been studied; however, most bioactives are water-insoluble, leading to low bioavailability and limiting food applications. The properties of bioactive foods can be controlled by their micro/nano-scale structure. Conventional preparations for bioactive foods are generally formulated by applying large-scale processing. Therefore, it is difficult to precisely control the structure of the products. Recently micro/nano engineering has been under development, as applied to bioactive foods. Emulsification is one of the important processes for food and other industries. Conventional emulsification equipment, such as the highpressure homogenizer, is used to produce emulsions. However, such obtained emulsions are polydispersed with a droplet diameter of 100 nm-100 mm. A new method for producing monodispersed emulsions using precisely-microfabricated microchannel (MC) arrays was proposed by Nakajima's group (1). MC emulsification is capable of producing monodispersed emulsions, which have been used to formulate the monodispersed microparticles and double emulsions. A nanochannel (NC) emulsification device has been also developed, in order to produce monodispersed sub-micron emulsions. Besides these micro/nano processing techniques, formulation and characterization of oil in water micro/nano emulsions or particles loaded with bioactives were studied, evaluating the physiochemical properties (2). In the presentation, the development of MC/NC emulsification, formulation and characterization of food nanoparticles and nanotechnology-based food will be discussed.Chaudhry will present "Applications and Implications of Nanotechnology for Food and Nutrition". The new developments in nanoscale sciences and technologies are promising to bring much innovation to the food sectors, and projected to revolutionize the food chain. Use of nano-sized ingredients in food products has raised concerns in regard to safety. Due to nanoscale dimensions and distinctive properties, the use of nanomaterials in food and health-food products poses a number of challenges, e.g. detection in food products, assessment of safety, and consumer acceptance. Chaudhry will discuss the known and projected applications of nanotechnology for food and related sectors, and will highlight the main challenges in r...

show abstract

Formation of Biocompatible Nanoparticles by Self-Assembly of Enzymatic Hydrolysates of Chitosan and Carboxymethyl Cellulose

Cited by 65 publications

References 24 publications

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Synthesis, Characterisation and Cytotoxicity of Polyoxometalate/Carboxymethyl Chitosan Nanocomposites

Formation of chitosan-fucoidan nanoparticles and their electrostatic interactions: Quantitative analysis

Nano-Science-Engineering-Technology Applications to Food and Nutrition

Contact Info

Product

Resources

About