Formation of an Antral Follicle–like Structure of Bovine Cumulus–Oocyte Complexes Embedded Individually or in Groups in Collagen Gels

Абакушина, Е. В.; Morita, Yasuhiro; Kaedei, Yukine; Tanihara, Fuminori; Namula, Zhao; Viet, VL; Otoi, Takeshige

doi:10.1111/j.1439-0531.2010.01684.x

Cited by 3 publications

(5 citation statements)

References 34 publications

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“…In a previous study, we observed that almost all oocytes had undergone the GVBD stage, irrespective of AFLS formation, when GV-stage oocytes with unknown oocyte diameter from follicles 1 to 5 mm in diameter were cultured for 14 days in collagen gel (Abakushina et al, 2011). In the present study, similarly, COCs aspirated from follicles 1-5 mm diameter were cultured for 8 days using the same culture method.…”

Section: Discussionmentioning

confidence: 79%

“…The aim of this experiment was to evaluate the effect of dbcAMP on the formation of the AFLS and on the meiotic status of oocytes cultured for 8 days. In a previous study, we found that the mean diameters of AFLS in the group culture method were smaller than those in the individual culture method, but more COCs formed AFLS in the group culture method (Abakushina et al, 2011). Therefore, the COCs were embedded in collagen gels by the group culture method (3 COCs per gel) and then cultured in TCM199 culture medium supplemented with 0.5, 1.0 or 2.0 mM of dbcAMP (Sigma-Aldrich) for 8 days.…”

Section: Hormonal Assessmentmentioning

confidence: 99%

“…Bovine oocytes were collected and cultured according to a previously reported method (Abakushina et al, 2011). Briefly, bovine ovaries were obtained from a slaughterhouse, and COCs were aspirated from follicles 1-5 mm in diameter using an 18-gauge needle connected to a 5-mL disposable syringe.…”

Section: Collection and Collagen-gel Embedding Culture Of Bovine Oocytesmentioning

confidence: 99%

“…The meiotic status of oocytes cultured for 8 days was examined according to the method described by Abakushina et al (2011). The AFLS formation relates to the proliferation of cumulus/granulosa cells surrounding oocytes and the viability of oocytes (Harada et al, 1997).…”

Section: Measurement Of Diameter Of Afls and Meiotic Status Of Oocytesmentioning

confidence: 99%

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Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Ono

Takaoka

Morita

et al. 2015

Acta Veterinaria Hungarica

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This study evaluated the effect of dibutyryl cyclic adenosine monophosphate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus-oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4-8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5-2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1-100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Müllerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.Key words: Antrum-like structures, cAMP, cumulus cells, gap junction, follicular formation Three-dimensional (3-D) culture of cumulus-oocyte complexes (COCs) may provide a valuable approach for studying the regulation of follicular development. When the 3-D organisation of cumulus granulosa cells around the oocyte was maintained during oocyte culture, COCs formed an antral follicle-like structure (AFLS) and grew in vitro (Harada et al., 1997). The formation of an AFLS is dependent on the proliferation and differentiation of cumulus granulosa

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Section: Discussionmentioning

confidence: 79%

Section: Hormonal Assessmentmentioning

confidence: 99%

Section: Collection and Collagen-gel Embedding Culture Of Bovine Oocytesmentioning

confidence: 99%

Section: Measurement Of Diameter Of Afls and Meiotic Status Of Oocytesmentioning

confidence: 99%

See 2 more Smart Citations

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Ono

Takaoka

Morita

et al. 2015

Acta Veterinaria Hungarica

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show abstract

“…The diameters of oocytes with AFLS formation were measured before and after growth culture for 8 days. The meiotic stage in the oocytes were analyzed by staining with the DNA-binding fluorochrome bisbenzimide (Hoechst 33342) that was previously described by Abakushina et al (9).…”

Section: Methodsmentioning

confidence: 99%

Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles

Morita

Karja

Kim

et al. 2015

Animal Biotechnology

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Fragmin/protamine microparticles (F/P MPs) approximately 0.5-1 µM in diameter were prepared by the simple mixing of fragmin with protamine. This study investigated the effects of F/P MP-containing collagen gels as a hormone carrier on the formation of antral follicle-like structures and on the development of growing bovine oocytes. The supplementation of F/P MPs in collagen gels contributed to the beneficial effects of follicle stimulating hormone (FSH) on the formation and size of antral follicle-like structures. The F/P MPs may serve as potential hormone carriers for the growth of cultured bovine oocytes from early antral follicles.

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Towards a 3D culture of mouse ovarian follicles

Belli¹,

Vigone²,

Merico³

et al. 2012

Int. J. Dev. Biol.

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The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.

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Formation of an Antral Follicle–like Structure of Bovine Cumulus–Oocyte Complexes Embedded Individually or in Groups in Collagen Gels

Cited by 3 publications

References 34 publications

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles

Towards a 3D culture of mouse ovarian follicles

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