Formation and stability of a suspended biomimetic lipid bilayer on silicon submicrometer-sized pores

Simon, Anne; Girard-Egrot, Agnès; Sauter, F.; Pudda, C.; Picollet-D’hahan, Nathalie; Blum, Loı̈c J.; Chatelain, François; Fuchs, Alexandra

doi:10.1016/j.jcis.2006.11.050

Cited by 61 publications

(55 citation statements)

References 38 publications

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“…One of the most intensively investigated solid supports for freely suspended lipid bilayers is a porous membrane with a pore diameter ranging from B10 to B100 nm (for example, anodic aluminumoxide, 49 polycarbonate membranes 50 and photolithographically generated silicon membranes). 51,52 Depending on the surface of the porous supports, the bilayer is formed via the rupture of large or giant unilamellar vesicles and painting of lipids dissolved in organic solvent (Figure 4a). 53 Such configurations are similar to the black lipid membrane in which planar lipid bilayers are formed across Teflon apertures with hole diameters of 0.1-1 mm.…”

Section: Lipid-1d-nanostructure Hybridsmentioning

confidence: 99%

“…A lithographically generated nanopore array in a silicon-based inorganic thin film has been suggested as an alternative platform to overcome these intrinsic limitations of anodic aluminumoxide supports. 51,52,55 In an attempt to maximize the stability of a free-standing lipid bilayer, the effect of such chemical and structural parameters as the pore diameter of silicon and silicon nitride nanopore supports was investigated with atomic force microscopy 52,53,56 ( Figure 4b) and impedance spectroscopy. 55 The results show that, in general, the bilayers suspended on the smaller pore arrays are more stable over time, and the aging stability of the lipid membranes is subject to the bilayer chemistry.…”

Section: Lipid-1d-nanostructure Hybridsmentioning

confidence: 99%

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Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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Cell membranes contain a variety of lipids and functional proteins that offer active platforms that organize the membrane components into functional assemblies and perform biologically important reactions. The dynamic and complex nature of the membranes makes them attractive materials, but at the same time, researchers report substantial experimental uncertainty in controlling the membrane reactions and extracting valuable information. The fascinating new structures and properties of nanomaterials could be utilized in addressing aforementioned issues, and the design and synthesis of lipid-nanostructure hybrids could be beneficial to the research areas in lipid-membrane biotechnology and nanobiotechnology. These hybrid structures possess dimensions that are comparable to that of biological molecules and structures and physicochemical properties that arise from both lipids and nanomaterials. Therefore, lipid-nanostructure hybrids offer additional options for control of the synthesized structures, provide new insight in understanding nanostructures and biological systems and allow the mimicking of functional subcellular membrane components and monitoring of the membrane-associated reactions in a highly sensitive and controllable manner. In this review, we present recent advances in the synthesis of various lipid-nanostructure hybrids and the application of these structures in biotechnology and nanotechnology. We further describe the scientific and practical applications of lipid-nanostructure hybrids for detecting membrane-targeting molecules, interfacing nanostructures with live cells and creating membrane-mimicking platforms to investigate various intercellular processes.

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Section: Lipid-1d-nanostructure Hybridsmentioning

confidence: 99%

Section: Lipid-1d-nanostructure Hybridsmentioning

confidence: 99%

Lipid-nanostructure hybrids and their applications in nanobiotechnology

Lee

Nam

2013

NPG Asia Mater

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“…6 There is a scale discrepancy between the micrometer-thick septa and nanometer-thick BLMs, which may result in an instability of the BLMs. When free-standing BLMs are spanned over nanopores, 11,12,25 or suspended in nanometer-thick substrates, 21 the membrane stability will presumably be enhanced. We have examined BLM formation across anodic porous alumina films (Fig.…”

Section: Fabrication and Blm Formationmentioning

confidence: 99%

Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities

et al. 2012

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“…A lipid monolayer is held at defined tension at the interface, which maximizes and controls the packing density. Using a hydrophilic substrate and starting in the solution, a lipid monolayer can be deposited onto the moving substrate (Simon et al 2007). …”

Section: Preparation Methods For Supported Lipid Membranesmentioning

confidence: 99%