Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities

Hirano‐Iwata, Ayumi; Oshima, Azusa; Mozumi, Hideki; Kimura, Yasuo; Niwano, Michio

doi:10.2116/analsci.28.1049

Cited by 17 publications

(13 citation statements)

References 46 publications

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“…[29][30][31][32][33][34] In this method, a lipid bilayer is formed by folding up two monolayers simultaneously, which spreads on the water surface, so that the organic solvent involved in BLMs becomes much less when compared with other methods such as the ''painting'' method or the DIB method. [29][30][31][32][33][34] In this method, a lipid bilayer is formed by folding up two monolayers simultaneously, which spreads on the water surface, so that the organic solvent involved in BLMs becomes much less when compared with other methods such as the ''painting'' method or the DIB method.…”

Section: Fabrication Of Blms Using the Folding Methodsmentioning

confidence: 99%

Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

et al. 2015

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Non-invasive measurement of the membrane tension of free-standing black lipid membranes (BLMs), with sensitivity on the order of μN m(-1), was achieved using laser-induced surface deformation (LISD) spectroscopy. A BLM was vertically formed via the folding method and aqueous phases with different refractive indices were added on each side in order to induce radiation pressure by a laser beam. The dynamic response of the deformed BLMs was measured under periodic intensity modulation and their tensions could be estimated. The dependence of membrane tension on the cholesterol concentration of BLMs composed of phosphatidylcholine and phosphatidylethanolamine was investigated, with the membrane tension increasing from 1.3 μN m(-1) to 68.1 μN m(-1) when the cholesterol concentration increased from zero to 33%. These tension values are much smaller than some of those previously reported, because this method does not suppress membrane fluctuation unlike other conventional methods. Our LISD system can be a promising tool for the measurement of membrane tension in BLMs.

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Section: Fabrication Of Blms Using the Folding Methodsmentioning

confidence: 99%

Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

et al. 2015

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“…The key feature of this approach is that the area of the stable BLMs is still large, which is suitable for the incorporation of ion channel proteins via proteoliposome fusion. The activities of proteoliposome‐delivered ion channels, such as acetylcholine receptor channel, KcsA potassium channel, and human ether‐a‐go‐go related gene (hERG) channel, were successfully recorded with the BLMs formed in the tapered apertures, showing promise for use as a platform for ion channel drug screening.…”

Section: Free‐standing Blms In Microfabricated Devicesmentioning

confidence: 99%

“…The use of a nonvolatile solvent has been a common approach for improving BLM stability . Such solvents increase the hydrophobicity of the pore rim and permit the lipids near the edge of the pore to become appropriately oriented .…”

Section: Free‐standing Blms In Microfabricated Devicesmentioning

confidence: 99%

“…In another approach, proteoliposomes are first fused to a phospholipid monolayer at the air/water interface (Figure b) and the channel‐containing lipid monolayer is then folded with another lipid monolayer to from BLMs containing ion channels . Based on the combination of these conventional approaches and BLMs suspended in microfabricated chips or gel‐supported BLMs, single‐channel activities of eukaryotic hERG, the rat N ‐methyl‐ d ‐aspartate receptor (NMDAR), the human glycine receptor, and BK channels were recorded (Table ). DIBs have also been coupled with conventional vesicle fusion.…”

Section: Incorporation Of Ion Channels Into Blmsmentioning

confidence: 99%

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Micro‐ and Nano‐Technologies for Lipid Bilayer‐Based Ion‐Channel Functional Assays

Hirano‐Iwata

Ishinari

Yamamoto

et al. 2015

Chemistry An Asian Journal

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Ion channel proteins provide gated pores that allow ions to passively flow across cell membranes. Owing to their crucial roles in regulating transmembrane ion flow, ion channel proteins have attracted the attention of pharmaceutical investigators as drug targets for use in the studies of both therapeutics and side effects. In this review, we discuss the current technologies that are used in the formation of ion channel-integrated bilayer lipid membranes (BLMs) in microfabricated devices as a potential platform for next-generation drug screening systems. Advances in BLM fabrication methodology have allowed the preparation of BLMs in sophisticated formats, such as microfluidic, automated, and/or array systems, which can be combined with channel current recordings. A much more critical step is the integration of the target channels into BLMs. Current technologies for the functional reconstitution of ion channel proteins are presented and discussed. Finally, the remaining issues of the BLM-based methods for recording ion channel activities and their potential applications as drug screening systems are discussed.

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“…6,7 More recently, mechanically stable bilayers have been be exploited, which can be formed, often semi-automatically, by virtue of advances in the bilayer formation technique. 8,9 Therefore, the design of transmembrane signaling associated with stable planar bilayer membranes will be interesting for constructing sophisticated biosensors that respond to a variety of targets.…”

Section: Introductionmentioning

confidence: 99%

A Planar Bilayer Lipid Membrane Sensor Using a Miniaturized Auto-patch System

et al. 2017

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Stable Lipid Bilayers Based on Micro- and Nano-Fabrication as a Platform for Recording Ion-Channel Activities

Cited by 17 publications

References 46 publications

Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

Measurement of membrane tension of free standing lipid bilayers via laser-induced surface deformation spectroscopy

Micro‐ and Nano‐Technologies for Lipid Bilayer‐Based Ion‐Channel Functional Assays

A Planar Bilayer Lipid Membrane Sensor Using a Miniaturized Auto-patch System

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