Formation and Characterization of Reversed Micelles Composed of Phospholipids and Fatty Acids

Sugiura, Shinji; Ichikawa, Sosaku; Sano, Yoh; Nakajima, Mitsutoshi; Liu, Xin Qi; Seki, Minoru; Furusaki, Shintaro

doi:10.1006/jcis.2001.7703

Cited by 13 publications

(9 citation statements)

References 27 publications

(37 reference statements)

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“…The bilayer‐forming lipids in the oil phase were dissolved in hexane at a relatively high concentration of 5% as the total concentration (PtdCho, C and OLA at 25 mM each). These amphiphilic molecules are known to form reversed micelles in nonpolar organic solvent such as hexane (Ichikawa et al, ; Sugiura et al, ; Walde et al, ). Our previous report (Kuroiwa et al, ) and this study showed that water and calcein are solubilized in hexane, and the fact that reversed‐micellar transport has been confirmed.…”

Section: Resultsmentioning

confidence: 99%

Lipid Vesicle Preparation Using W/O/W Multiple Emulsions Via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials

Ossai

Kuroiwa

Horikoshi

et al. 2019

J Americ Oil Chem Soc

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We studied the effects of emulsifiers on the entrapment yields of a hydrophilic material, calcein, in lipid vesicles formed using the multiple emulsion method. The primary emulsion (W 1 /O) was prepared via sonication while the secondary emulsification that produced the W 1 /O/W 2 multiple emulsions was achieved using the microchannel (MC) emulsification technique. The emulsifiers used in the continuous (W 2 ) phase were Tween ® 80, Pluronic ® F68, and bovine serum albumin (BSA). Lipid vesicles formed via solvent evaporation of the multiple emulsion droplets had an average diameter of about 180 nm, similar to the size of the water droplets in the primary W 1 /O emulsions. The entrapment yields of calcein increased with decreasing concentrations of Tween ® 80 but decreased with decreasing concentrations of Pluronic ® F68 and BSA. The effects of type and concentration of emulsifier were considered in relation to three possible mechanisms: (i) destabilization/solubilization of lipid bilayers by emulsifiers, (ii) reversed-micellar transport of W 1 contents from internal to external water phases through the O phase, and (iii) release of inner water (W 1 ) contents into the continuous (W 2 ) phase via the instability of W 1 /O/W 2 and leakage of W 1 contents. Using the food grade emulsifier Tween ® 80 at a low concentration of about 0.1 or 0.05 wt%, calcein as a model hydrophilic material could be efficiently entrapped (ca. 80%) in homogenously dispersed lipid vesicles.

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Section: Resultsmentioning

confidence: 99%

Lipid Vesicle Preparation Using W/O/W Multiple Emulsions Via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials

Ossai

Kuroiwa

Horikoshi

et al. 2019

J Americ Oil Chem Soc

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“…This phenomenon can be explained by the inadequacy of water in the lecithin-hexane system to provide the required central core of micelles, as the commercial soy lecithin contained only 0.24% moisture. When the lecithin-to-hexane mass ratio increased, the resulting lower PL concentration led to the occurrence of single PL molecules or small aggregates, as reflected by the large proportion of micelles around 1 nm [15]. When the proportion of hexane decreased, the concentration of PL increased, and the small PL molecules began to interact.…”

Section: Effect Of Solvent Ratiomentioning

confidence: 95%

Preparation of Deoiled Soy Lecithin by Ultrafiltration

Liu

et al. 2011

J Americ Oil Chem Soc

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Deoiling of soy lecithin through ultrafiltration (UF) using an inorganic ceramic membrane was examined in a favorable solvent medium (hexane). Phospholipid (PL) reverse micelles with an average particle size of 9.8 nm were prepared in a 1:3 soy lecithin/hexane system (m/m) with 9% water added at a temperature of 25°C. Consequently, an inorganic ceramic membrane with a pore size of 5 nm was selected. UF was employed in continuous diafiltration mode at transmembrane pressure (TMP) of 0.25 MPa, and a final deoiled lecithin product with a high acetone insoluble (AI) content of 96.32% was obtained at a yield of around 84%.

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“…The influence of the nonpolar solvent along with other chemical components, i.e. water and additives, on phospholipid self-assembly is illustrated by the large variety of lecithin morphologies reported in literature (13,19,56). It is worth noting that very subtle differences in the oil phase affect the formed reverse micellar structures (36).…”

Section: Effect Of Increasing Water Concentration On Lecithin Self-asmentioning

confidence: 98%

Effect of temperature, water content and free fatty acid on reverse micelle formation of phospholipids in vegetable oil

Lehtinen

Nugroho

Lehtimaa

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Formation and Characterization of Reversed Micelles Composed of Phospholipids and Fatty Acids

Cited by 13 publications

References 27 publications

Lipid Vesicle Preparation Using W/O/W Multiple Emulsions Via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials

Lipid Vesicle Preparation Using W/O/W Multiple Emulsions Via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials

Preparation of Deoiled Soy Lecithin by Ultrafiltration

Effect of temperature, water content and free fatty acid on reverse micelle formation of phospholipids in vegetable oil

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