2022
DOI: 10.3390/ani12030313
|View full text |Cite
|
Sign up to set email alerts
|

Form of Supplemental Selenium Affects the Expression of mRNA Transcripts Encoding Selenoproteins, and Proteins Regulating Cholesterol Uptake, in the Corpus Luteum of Grazing Beef Cows

Abstract: Selenium (Se)-deficient soils necessitate supplementation of this mineral to the diet of forage-grazing cattle. Functionally, Se is incorporated into selenoproteins, some of which function as important antioxidants. We have previously shown that the source of supplemental Se; inorganic (sodium selenite or sodium selenate; ISe), organic (selenomethionine or selenocysteine; OSe) or 1:1 mix of ISe and OSe (MIX), provided to Angus-cross cows affects concentrations of progesterone (P4) during the early luteal phase… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
7
0

Year Published

2022
2022
2023
2023

Publication Types

Select...
7

Relationship

2
5

Authors

Journals

citations
Cited by 8 publications
(7 citation statements)
references
References 131 publications
0
7
0
Order By: Relevance
“…Consequently, we accept the hypothesis that the pituitary and hepatic selenoprotein mRNA content of steers raised on MIX versus ISe forms of supplemental Se, and consumed MIX versus ISe forms of s u p p l e m e n t a l S e , wh i l e u n d e r go i n g s u m m e r -l o n g consumption of toxic endophyte-infected tall fescue, differed. When these findings are considered with our recent report that the mRNA content of GPX6, selenoprotein P, selenoR, and selenoV is increased in the corpus luteum (another high metabolic activity tissue) of MIX versus ISe replacement beef heifers (Carr et al, 2022), it seems reasonable to conclude that supplementation of cattle with different forms of Se affects the expressed selenoprotein transcriptome.…”
Section: Complement and Abundance Of Selenoprotein Mrna Expressed By ...mentioning
confidence: 67%
“…Consequently, we accept the hypothesis that the pituitary and hepatic selenoprotein mRNA content of steers raised on MIX versus ISe forms of supplemental Se, and consumed MIX versus ISe forms of s u p p l e m e n t a l S e , wh i l e u n d e r go i n g s u m m e r -l o n g consumption of toxic endophyte-infected tall fescue, differed. When these findings are considered with our recent report that the mRNA content of GPX6, selenoprotein P, selenoR, and selenoV is increased in the corpus luteum (another high metabolic activity tissue) of MIX versus ISe replacement beef heifers (Carr et al, 2022), it seems reasonable to conclude that supplementation of cattle with different forms of Se affects the expressed selenoprotein transcriptome.…”
Section: Complement and Abundance Of Selenoprotein Mrna Expressed By ...mentioning
confidence: 67%
“…Using an extensively reported model [10,28,29,42,43], we studied the effects of supplemental form of Se as ISe (the industry standard) or a 1:1 mixture of ISe to OSe (MIX) on grazing beef cows and heifers and observed significant effects on the CL [10] and endometrium [34], which laid the premise for the present study. This experiment had two specific objectives.…”
Section: Discussionmentioning
confidence: 99%
“…Real-time PCR (qPCR) was used to quantify the relative abundance of mRNA in CAR samples for identified genes that differed according to the RNA-Seq analysis and to characterize mRNA transcript abundance of selenoproteins and related receptors in CAR at MRP using a technique that is routinely used in our laboratory [10,34,43]. Approximately 1 µg of RNA from each sample was reverse-transcribed into cDNA using SuperScript IV VILO Master Mix with ezDNAse Enzyme (Invitrogen by Thermo Fisher Scientific, Vilnius, Lithuania).…”
Section: Real-time Pcr Analysismentioning
confidence: 99%
See 1 more Smart Citation
“…Selenium supplementation was also found to affect progesterone levels of cows and sheep across different phases of estrus [ 284 , 285 ] and gestation [ 286 , 287 ]. Further investigation into relevant mRNA transcript levels in the corpus luteum uncovered no change in transcripts involved in progesterone synthesis, but yielded an increase in transcripts ( Ldlr and Hsl ) involved in the regulation of cholesterol availability [ 288 ]. This might suggest that selenium-induced effects on progesterone production in the corpus luteum are consequences of changes in cholesterol availability, rather than directly influencing progesterone synthesis.…”
Section: Signals From Brain To Bodymentioning
confidence: 99%