Forensics: Food Authentication Using MtDNA

Murugaiah, Chandrika

doi:10.15226/jnhfs.2015.00153

Cited by 6 publications

(4 citation statements)

References 101 publications

(157 reference statements)

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“…In Iraq gastrointestinal parasitic protozoal infections are commonly and widely distributed because of the low level of environmental and personal hygiene due to improper disposal of humans and animals excreta. The infections with Giardia and Cryptosporidium occur by the fecal-oral route [46,47]. Cryptosporidium is food and waterborne disease-causing diarrhea in both humans and animals; children are highly exposed to infection due to their direct contact with pets and with insufficient hygiene habits; and may lead to death of small children, adults and immunocompromised people (AIDS patients) [48,49].…”

Section: Public Health Importance Of Cryptosporidiosis and Giardiasismentioning

confidence: 99%

Cryptosporidium and Giardia Infection in Pet Animals: A review Study in Iraq

Alhayali

Al-Taee

Alneema

2021

Egyptian Journal of Veterinary Sciences

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C ryptosporidium parvum and Giardia lamblia are the most common zoonotic intestinal protozoan parasites causing diarrheal diseases Cryptosporidiosis and Giardiasis are significant health concerns in both humans and animals; for this reason, these parasites acquired public and veterinary importance. Dogs and cats are susceptible to infection and play an important role in transmitting these protozoans to human. Recently in Iraq, there is an interest in keeping pet animals and an increase of stray cats and dogs which accompany livestock. Some people started to attract stray cats through feeding and became like house animals. This literature review focused on the results of Iraqi researchers to overview the importance prevalence of these parasites on both human and animals. This literature review recommended to educate pet owners especially children and follow veterinarians' instructions, to control and prevent transmission of these zoonotic diseases and future researches should focus and investigate from these finding and highlight the role of domestic animals in transmission of these diseases.

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Section: Public Health Importance Of Cryptosporidiosis and Giardiasismentioning

confidence: 99%

Cryptosporidium and Giardia Infection in Pet Animals: A review Study in Iraq

Alhayali

Al-Taee

Alneema

2021

Egyptian Journal of Veterinary Sciences

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“…The effectiveness of multiplex PCR depends on primer design and intra-species conserved regions, such as mitochondrial DNA (mtDNA), are preferable to nuclear DNA targets ( Mohamad et al, 2013 ) simply because they are more specific. In studying molecular traceability of species origin, mitochondrial genes have been targeted because of their abundance per cell and maternal inheritance, which is advantageous for discrimination of closely related species ( Murugaiah et al, 2015 , Wang et al, 2006 ). The widely used mtDNA gene for phylogenic study is cytochrome b ( Cyt b ), which is highly conserved making it suitable for identifying intra- and inter-species ancestry even in processed foods ( Murugaiah et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…In studying molecular traceability of species origin, mitochondrial genes have been targeted because of their abundance per cell and maternal inheritance, which is advantageous for discrimination of closely related species ( Murugaiah et al, 2015 , Wang et al, 2006 ). The widely used mtDNA gene for phylogenic study is cytochrome b ( Cyt b ), which is highly conserved making it suitable for identifying intra- and inter-species ancestry even in processed foods ( Murugaiah et al, 2015 ). Several studies have been performed using multiplex PCR for mitochondrial genes for verification of meat species ( Hou et al, 2015 , Zarringhabaie et al, 2011 ) and identification of milk species ( Dalmasso et al, 2011 , Csikos et al, 2016 , López-Calleja et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of species adulteration in meat products and Mozzarella-type cheeses using duplex PCR of mitochondrial cyt b gene: A food safety concern in Bangladesh

Khatun

Hossain

Munshi

et al. 2021

Food Chemistry: Molecular Sciences

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“…Various molecular techniques used for food authentication have been developed and reviewed [6,7]. These techniques are based on the DNA polymorphism between species and are classified into three types.…”

Section: Introductionmentioning

confidence: 99%

Authentication of Meat and Meat Products Using Triacylglycerols Profiling and by DNA Analysis

Hrbek

Zdeňková

Jilkova

et al. 2020

Foods

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Two alternative, complementary analytical strategies were successfully used to identify the most common meat species—beef, pork and chicken—in meat products. The first innovative high-throughput approach was based on triacylglycerols fingerprinting by direct analysis in real time coupled with high-resolution mass spectrometry (DART–HRMS). The second was the classic commonly used DNA analysis based on the use of nuclear or mitochondrial DNA in multiplex polymerase chain reaction (mPCR). The DART–HRMS method represents a rapid, high throughput screening method and was shown to have a good potential for the authentication of meat products. Nevertheless, it should be noted that due to a limited number of samples in this pilot study, we present here a proof of concept. More samples must be analyzed by DART–HRMS to build a robust classification model applicable for reliable authentication. To verify the DART–HRMS results, all samples were analyzed by PCRs. Good compliance in samples classification was documented. In routine practice under these conditions, screening based on DART–HRMS could be used for identification of suspect samples, which could be then examined and validated by accurate PCRs. In this way, saving of both labor and cost could be achieved. In the final phase, commercially available meat products from the Czech market were tested using this new strategy. Canned meats—typical Czech sausages and luncheon meats, all with declared content of beef, pork and chicken meat—were used. Compliance with the label declaration was confirmed and no adulteration was found.

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Forensics: Food Authentication Using MtDNA

Cited by 6 publications

References 101 publications

Cryptosporidium and Giardia Infection in Pet Animals: A review Study in Iraq

Cryptosporidium and Giardia Infection in Pet Animals: A review Study in Iraq

Detection of species adulteration in meat products and Mozzarella-type cheeses using duplex PCR of mitochondrial cyt b gene: A food safety concern in Bangladesh

Authentication of Meat and Meat Products Using Triacylglycerols Profiling and by DNA Analysis

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