This trial was aimed to determine the prevalence of renal coccidiosis in domestic geese and ducks in Nineveh governorate, Iraq. One hundred and thirty birds of both sexes and different ages were randomly purchased from many localities of Nineveh governorate during the period between 2017 and 2018. According to geese and ducks' age, the overall infection was 70% and 51.66%, respectively, 74.35% for geese and 53.19% for ducks <6 months, 64.51% and 46.15% for ≤7 months. Sex distribution of renal coccidiosis showed that 75.55%, 54.05% of female geese and ducks, and 60%, 47.82% of male geese and ducks were positive, respectively. Impression smears from kidneys for the presence of Eimeria truncata in geese showed that 49 (70 %) were positive, while 31 (51.66%) were positive for ducks. Cloacal fecal samples for Eimeria truncata oocytes' were tested by flotation technique revealed that 22.85% were positive for geese and 16.66% for ducks. The dimensions of Eimeria truncata development stages (schizonts, macrogametes, microgametes, and oocytes) were measured. The histological sections of the infected geese and ducks' kidneys showed the different developmental stages of the Eimeria truncata parasite with various pathological changes.
The current study included the possibility using fluorescent Acridine orange stain in the diagnosis of Babesia spp in cattle in Mosul city/Iraq, this dye is easily applied and takes no more than 2-5 minutes in the diagnosis of Babesia spp using of fluorescent microscope versus 45 minutes of Giemsa stain by using light microscope. The benefit of Acridine orange stain is in its uses in epidemiological and survey studies and for the control programs against Babesiosis but it is considered to be of little value in study of morphological features of small blood protozoa with low parasitemia comparing with the golden Giemsa stain in the study of morphological and specifications of Babesia spp.
The current study examined 100 small intestines collected randomly from sheep slaughtered in the abattoir and butcher's shops from different Mosul city / Iraq areas of both sexes (55 females, 45 males) and different ages. Moniezia expansa was diagnosed in 9 samples of intestines by studying the morphometric characteristics of these tapeworms, especially the mature segments, in which both the ovaries and vitelline glands appeared in the ring shape on either side of the body segments and the rosette-like shape of the interproglotidial glands. No significant difference was noticed between males and females of sheep in our study, and the infection rate was 10% in sheep less than a year old and older than two years, with no significant difference between the age groups. The results of the molecular analysis by using conventional polymerase chain reaction technique confirmed the diagnosis of these worms, which belong to the genus Moniezia, with a product reaction of 700 base pairs. The sequencing result shows two strains of Moniezia expanza, which isolated from Iraq (Moniezia expansa-Iraqi one and Moniezia expansa-Iraqi 2) were similar to each other had a significant distance to other strains. The study also showed that Moniezia expansa is different from the same species in other countries.
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