Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Panday, Raju; Jha, Dinesh Kumar; Thapa, Nirajan; Pokharel, Basant R.; Aryal, Nanda K.

doi:10.2174/1874402801407010006

Cited by 15 publications

(12 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason for obtaining different lengths was probably due to inhibition of sequencing reactions by individual matrices. Similar results were obtained by Kumar Gupta [24], who worked on stool samples, and also obtained short Cytb sequence fragments of 366, 374 and 503 bp [14,34,46].…”

Section: Discussionsupporting

confidence: 86%

“…We show that when using bone tissue, the primers used in this work for the Cytb gene fragment ampli cations work better, because rstly, they differentiate closely related species well and have the additional advantage that they can be used for many other mammalian species as well. Our research is con rmed by many studies, not only for the family Cervidae, but also other works on the identi cation of wild mammalian species [1,5,23,34,47,48].…”

Section: Discussionsupporting

confidence: 62%

“…At the beginning, it should be noted that DNA analysis of biological samples has become the standard practice in animal identi cation at various taxonomic levels. Different types of tissues, such as bones, blood, hair (fur), feathers, skin, meat (muscle sample), faecal, and others are often the subject of many studies in various DNA analysis laboratories [14,34].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Universal Fragment of mtDNA for Cervidae and Other Ungulate Species Identification

Filip

Strzała²,

Stępień

et al. 2020

Preprint

View full text Add to dashboard Cite

ObjectiveThe abundance of literature and many studies aimed at the identification of free-living animal species has helped to identify specific nucleotide marker sequences. In many cases, the best marker to distinguish species is the mitochondrial genome (mtDNA) or one of its fragments. In molecular analyses of Cervidae, biological material such as blood or muscle is easily obtained, as nearly all of these species are gaming animals.ResultsIn our research, we present the case study of successful species identification based on degraded samples of bone, with the use of short mtDNA fragments. We obtained a partial sequence of the mitochondrial cytochrome b (Cytb) gene for Capreolus capreolus, Dama dama, and Cervus elaphus, that can be used for species affiliation. The proposed methodology is helpful as a routine identification procedure for a variety of tissue sources, even in cases where the samples are degraded. The new sequences have been deposited in GenBank, enriching the existing Cervidae mtDNA base.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Universal Fragment of mtDNA for Cervidae and Other Ungulate Species Identification

Filip

Strzała²,

Stępień

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Plethora of instances using different molecular techniques are available for species-level identification (Hakki et al 2003;Asmelash et al 2017). In the recent past, the use of molecular markers (Cytochrome b, 12S ribosomal RNA, 16S ribosomal RNA, and Cytochrome oxidase c subunit I) also estimated as a successful tool for species identification due to the bearing of nucleotide variation among the species (Hsieh et al 2001;Panday et al 2014). Nevertheless, millions of gene sequences are presently available in global database (http://www.boldsystems.org, https://www.ncbi.nlm.nih.gov) and the online gene sequence similarity search engines are proved to be effective to examine the DNA data, generated from any biological sources.…”

Section: Introductionmentioning

confidence: 99%

Utility of mitochondrial DNA in wildlife forensic science: reliable identification of confiscated materials from Eastern India

Kumar

Chandra

Kundu

et al. 2019

Mitochondrial DNA Part B

View full text Add to dashboard Cite

The poachers adopted various cunning ways to modify the wildlife materials before commercialization. Thus, the confiscated wildlife materials is often a crime with no eyewitness and difficult for identification. However, accurate identification of the seized wildlife samples is vital for species identification, detection of trade origin and better conservation management. The study employed DNA sequencing based identification of suspected wildlife materials, received from forest departments in east India. We adopted sufficient safety measures while dealing with the samples and processed the entire job at the in-house facilities in Zoological Survey of India (ZSI), Kolkata. Among 21 confiscated wildlife materials, most of them are identified by Cytb gene, while one specimen by COI gene, and one by both COI and Cytb. All the confiscated amorphous samples are accurately detected by sequence similarity search (99%-100%), estimated genetic divergence, and return monophyly using NJ phylogenetic analysis. The study detected the ongoing illegal trade of five of east India's most threatened animals, B. gaurus, N. nebulosa, R. unicornis, L. olivacea, and P. molurus. The study further identified the illegal hunting of mammals (S. scrofa, B. indicus, M. muntjak, A. axis), avifauna (G. leucolophus), and herpetofauna (N. naja) in the region. The study affirms the efficacy of DNA based identification tools for detection of wildlife crime and forensic sciences.

show abstract

“…Moreover, many international organizations such as the Consortium for the Barcode of Life (CBOL) and the Barcode of Life Data System (BOLD) have been established to promote DNA barcoding as a global standard for species identification. In addition to taxonomy, DNA barcoding has recently been applied in various fields, including medicine and food science, forensics, and conservation biology [ 19 , 20 , 25 – 27 ]. In the field of TM, DNA barcoding has been widely used to authenticate herbal sources [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding for the Identification and Authentication of Animal Species in Traditional Medicine

Yang

Ding

Chen

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Animal-based traditional medicine not only plays a significant role in therapeutic practices worldwide but also provides a potential compound library for drug discovery. However, persistent hunting and illegal trade markedly threaten numerous medicinal animal species, and increasing demand further provokes the emergence of various adulterants. As the conventional methods are difficult and time-consuming to detect processed products or identify animal species with similar morphology, developing novel authentication methods for animal-based traditional medicine represents an urgent need. During the last decade, DNA barcoding offers an accurate and efficient strategy that can identify existing species and discover unknown species via analysis of sequence variation in a standardized region of DNA. Recent studies have shown that DNA barcoding as well as minibarcoding and metabarcoding is capable of identifying animal species and discriminating the authentics from the adulterants in various types of traditional medicines, including raw materials, processed products, and complex preparations. These techniques can also be used to detect the unlabelled and threatened animal species in traditional medicine. Here, we review the recent progress of DNA barcoding for the identification and authentication of animal species used in traditional medicine, which provides a reference for quality control and trade supervision of animal-based traditional medicine.

show abstract

Forensic Wildlife Parts and their Product Identification and Individualization Using DNA Barcoding

Cited by 15 publications

References 88 publications

Universal Fragment of mtDNA for Cervidae and Other Ungulate Species Identification

Universal Fragment of mtDNA for Cervidae and Other Ungulate Species Identification

Utility of mitochondrial DNA in wildlife forensic science: reliable identification of confiscated materials from Eastern India

DNA Barcoding for the Identification and Authentication of Animal Species in Traditional Medicine

Contact Info

Product

Resources

About