Foreign body response to subcutaneous biomaterial implants in a mast cell-deficient Kitw-Sh murine model

Avula, Mahender N.; Rao, Archana N.; McGill, Lawrence D.; Grainger, David W.; Solzbacher, Florian

doi:10.1016/j.actbio.2013.12.056

Cited by 58 publications

(69 citation statements)

References 36 publications

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“…6 show similar inflammatory responses and collagen deposition around masitinibreleasing CGM implants compared to control implants at 21 days. Masitinib's effect in targeting fibroblasts via mast cell-stabilization and through the inhibition of fibroblast growth factor receptor 3 (FGFR3) should pharmacologically result in reduced collagen production (Avula et al, 2014;Dubreuil et al, 2009). This anticipated pharmacology is apparently not observed.…”

Section: Discussionmentioning

confidence: 97%

“…The functionality of the sensors is also independent of oxygen availability, limiting the factors affecting sensor response in vivo (Feldman et al, 2003). PEG-PLGA microsphere composite coatings were designed to dissolve the PEG matrix within minutes of implantation to allow unhindered glucose access to the sensor surface (Avula et al, 2013;2014). This coating shows little significant effect on the sensors' glucose sensitivity: less than 10% change is observed in sensor signal outputs tested in vitro in a glucose standard before and after coating.…”

Section: Discussionmentioning

confidence: 98%

“…Tissue beds were fixed in 10% neutral buffered formalin and sent to Associated Research and University Pathologists (ARUP, USA) labs for slicing and staining with Hematoxylin and Eosin (H&E), and Masson's Trichrome (MTC) stains. Histologically prepared slides of various tissue sites were evaluated for fibrous encapsulation in number of layers around implant sites from multiple sites (n ¼5) on multiple sections (n ¼4) for each implant type (Avula et al, 2013;2014).…”

Section: Histological Evaluationmentioning

confidence: 99%

“…Sensors were coated with a soluble PEG matrix-PLGA microsphere composite thin film matrix formulation as described previously (Avula et al, 2013;2014). Briefly, PLGA microspheres containing masitinib were dispersed in aqueous PEG solution and the resulting formulation is coated around the commercial CGM sensors cryogenically using a 2-part aluminum mold, yielding a rapidly (few minutes) soluble PEG coating that disperses the drugloaded PLGA microspheres around the implant peripheral tissue site at distances of several hundred micrometers (Avula et al, 2013;2014). Control implant coatings were prepared with a similar process with identical ingredients (PEG, PLGA, etc.)…”

Section: Glucose Sensor Surface Modification Drug Loading and In VImentioning

confidence: 99%

“…We propose that its pharmacology could be exploited to benefit CGM function. We use drug formulated into a microsphere delivery system released locally from CGM sensors implanted subcutaneously in wild type C57BL/6 mice to monitor CGM functional responses in situ for 21 days (Avula et al, 2013;2014).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Local release of masitinib alters in vivo implantable continuous glucose sensor performance

Avula

Jones

Rao

et al. 2016

Biosensors and Bioelectronics

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Histological Evaluationmentioning

confidence: 99%

Section: Glucose Sensor Surface Modification Drug Loading and In VImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Local release of masitinib alters in vivo implantable continuous glucose sensor performance

Avula

Jones

Rao

et al. 2016

Biosensors and Bioelectronics

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Extracellular Matrix‐Based Biohybrid Materials for Engineering Compliant, Matrix‐Dense Tissues

Bracaglia

Fisher

2015

Adv Healthcare Materials

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An ideal tissue engineering scaffold should not only promote, but take an active role in, constructive remodeling and formation of site appropriate tissue. ECM-derived proteins provide unmatched cellular recognition, and therefore influence cellular response towards predicted remodeling behaviors. Materials built with only these proteins, however, can degrade rapidly or begin too weak to substitute for compliant, matrix-dense tissues. The focus of this review is on biohybrid materials that incorporate polymer components with ECM-derived proteins, to produce a substrate with desired mechanical and degradation properties, as well as actively guide tissue remodeling. Materials are described through four fabrication methods: (1) polymer and ECM-protein fibers woven together, (2) polymer and ECM proteins combined in a bilayer, (3) cell-built ECM on polymer scaffold, and (4) ECM proteins and polymers combined in a single hydrogel. Scaffolds from each fabrication method can achieve characteristics suitable for different types of tissue. In vivo testing has shown progressive remodeling in injury models, and suggests ECM-based biohybrid materials promote a prohealing immune response over single component alternatives. The prohealing immune response is associated with lasting success and long term host maintenance of the implant.

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Polymer scaffold architecture is a key determinant in mast cell inflammatory and angiogenic responses

Abebayehu

Spence

McClure

et al. 2019

J Biomedical Materials Res

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Implanted polymer scaffolds can induce inflammation leading to the foreign body response (FBR), fibrosis, and implant failure. Thus, it is important to understand how immune cells interact with scaffolds to mitigate inflammation and promote a regenerative response. We previously demonstrated that macrophage phenotype is modulated by fiber and pore diameters of an electrospun scaffold. However, it is unclear if this effect is consistent among other innate immune cells. Mast cells are inflammatory sentinels that play a vital role in the FBR of implanted biomaterials, as well as angiogenesis. We determined if altering electrospun scaffold architecture modulates mast cell responses, with the goal of promoting regenerative cell-scaffold interactions. Polydioxanone (PDO) scaffolds were made from 60 mg/mL or 140 mg/mL PDO solutions, yielding structures with divergent fiber and pore diameters. Mouse mast cells plated on these scaffolds were activated with IL-33 or lipo-polysaccharide (LPS). Relative to the 60 mg/mL scaffold, 140 mg/mL scaffolds yielded less IL-6 and TNF, and greater VEGF secretion. Pores >4–6 μm elicited less IL-6 and TNF secretion. IL-33-induced VEGF regulation was more complex, showing effects of both pore size and fiber diameter. These data indicate parameters that can predict mast cell responses to scaffolds, informing biomaterial design to increase wound healing and diminish implant rejection.

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Foreign body response to subcutaneous biomaterial implants in a mast cell-deficient Kitw-Sh murine model

Cited by 58 publications

References 36 publications

Local release of masitinib alters in vivo implantable continuous glucose sensor performance

Local release of masitinib alters in vivo implantable continuous glucose sensor performance

Extracellular Matrix‐Based Biohybrid Materials for Engineering Compliant, Matrix‐Dense Tissues

Polymer scaffold architecture is a key determinant in mast cell inflammatory and angiogenic responses

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