The presence of zoonotic pathogens in bioterrorism poses a serious danger to worldwide health and safety. Zoonotic diseases, which can be transmitted from animals to humans, are an attractive choice for individuals looking to cause deliberate harm. This summary explores how zoonotic diseases could be used for bioterrorism, emphasizing the dangers and difficulties involved. Zoonotic organisms like bacteria, viruses, and parasites can be readily acquired from natural sources, presenting a wide range of potential biological weapons. Concealing the source of the pathogen in animals makes it challenging to track, contributing to the covert nature of these acts of bioterrorism. Controlling the spread and identifying deliberate outbreaks of zoonotic diseases, which are transmitted by animal vectors, is challenging due to their complex transmission dynamics. The deliberate use of zoonotic pathogens in bioterrorism is intended to induce sickness, fatalities, or fear in human communities. Malign individuals may attempt to enhance the risk and resistance of zoonotic agents, intensifying their impact on public health and straining healthcare systems. Identifying and dealing with zoonotic bioterrorism poses significant challenges. Recognizing intentional events late can slow down quick reactions. The interconnected nature of human, animal, and environmental health emphasizes the importance of using a One Health approach, which encourages cooperation to address and minimize the effects of purposeful release of disease-causing agents from animals. In order to prevent the deliberate transmission of zoonotic diseases, we require improved monitoring, biosecurity measures, and international collaboration. Global cooperation is essential to tackle the cross-border danger, enhance biosafety measures, exchange crucial data, and reinforce response capacity. It is essential to comprehend and address the possible impact of zoonotic diseases in the event of bioterrorism in order to safeguard public health and global security.