Aims: To investigate the phytochemical composition and effect of temperature on the proximate and mineral composition of Zingiber officinale. Study Design: Activity directed phytochemical screening, proximate analysis and mineral composition investigation of Z. officinale rhizomes using in vitro methods. Place and Duration of Study: Medicinal Plants Section, Bioresources Development Centre, Ogbomoso, Nigeria between May and November, 2016. Methodology: Fresh rhizome of Z. officinale was milled, extracted with absolute ethanol and screened for phytochemicals. Proximate and mineral analyses were carried out at various temperatures; room temperature (28°C; control), 40, 50 and 60°C.