Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products

Bruno, Antonia; Sandionigi, Anna; Agostinetto, Giulia; Bernabovi, Lorenzo; Frigerio, Jessica; Casiraghi, Maurizio; Labra, Massimo

doi:10.3390/genes10030248

Cited by 46 publications

(30 citation statements)

References 40 publications

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“…Moreover, the DNA metabarcoding approach has also been demonstrated to be useful for quantitative analysis. In fact, differences in sequence reads abundance between species can be used to infer the corresponding differences in species abundance in a food sample [ 116 ].…”

Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

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In the last decades, the demand for molecular tools for authenticating and tracing agri-food products has significantly increased. Food safety and quality have gained an increased interest for consumers, producers, and retailers, therefore, the availability of analytical methods for the determination of food authenticity and the detection of major adulterations takes on a fundamental role. Among the different molecular approaches, some techniques such as the molecular markers-based methods are well established, while some innovative approaches such as isothermal amplification-based methods and DNA metabarcoding have only recently found application in the agri-food sector. In this review, we provide an overview of the most widely used molecular techniques for fresh and processed agri-food authentication and traceability, showing their recent advances and applications and discussing their main advantages and limitations. The application of these techniques to agri-food traceability and authentication can contribute a great deal to the reassurance of consumers in terms of transparency and food safety and may allow producers and retailers to adequately promote their products.

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Section: Molecular Approaches To Agri-food Analysismentioning

confidence: 99%

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Fanelli

Mascio

Miazzi

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…This method has also been used to look at Echinacea products [13], but with the same lack of differentiation. Similarly, metabarcoding has been used on plant species in foods as well [14], but with the same limitations based on the constrained resolution of species.…”

Section: Hplc-uv Metabarcoding and Genome Skims Of Botanical Dietarymentioning

confidence: 99%

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

et al. 2021

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The use of DNA-based methods to authenticate botanical dietary supplements has been vigorously debated for a variety of reasons. More comparisons of DNA-based and chemical methods are needed, and concordant evaluation of orthogonal approaches on the same products will provide data to better understand the strengths and weaknesses of both approaches. The overall application of DNA-based methods is already firmly integrated into a wide array of continually modernizing stand alone and complementary authentication protocols. Recently, the use of full-length chloroplast genome sequences provided enhanced discriminatory capacity for closely related species of Echinacea compared to traditional DNA barcoding approaches (matK and rbcL). Here, two next-generation sequencing approaches were used: (1) genome skimming and (2) PCR amplicon (metabarcoding). The two genetic approaches were then combined with HPLC-UV to evaluate 20 commercially available dietary supplements of Echinacea representing “finished” products. The trade-offs involved in different DNA approaches were discussed, with a focus on how DNA methods support existing, accepted chemical methods. In most of the products (19/20), HPLC-UV suggested the presence of Echinacea spp. While metabarcoding was not useful with this genus and instead only resolved 7 products to the family level, genome skimming was able to resolve to species (9) or genus (1) with the 10/20 products where it was successful. Additional ingredients that HPLC-UV was unable to identify were also found in four products along with the relative sequence proportion of the constituents. Additionally, genome skimming was able to identify one product that was a different Echinacea species entirely.

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“…This is particularly important since the quality, reliability, and consistency of analytical results are used to judge the quality and authenticity of a product. DNA-based methods for molecular diagnosis are some of the most promising prospective standards for quality confidence, though they require further validation (Bruno et al, 2019;Newmaster et al, 2019). Validating DNA metabarcoding is however challenging.…”

Section: Bidens Andicola 2 Ptu_232mentioning

confidence: 99%

DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika

Raclariu-Manolică

Anmarkrud

Kierczak³

et al. 2021

Front. Plant Sci.

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Herbs and spices are some of the most vulnerable products in terms of fraud and adulteration in the food sector. Although standard analytical methods are accurate for quality control of specific lead or marker compounds, they cannot accurately assess the entire species composition of many marketed products. Complementary analytical approaches are thus often used for comprehensive screening of herbs and spices. In this study we evaluate DNA metabarcoding for the identification and authentication of 62 products, containing basil, oregano, and paprika collected from different retailers and importers in Norway. Our results show varying degrees of discrepancy between the constituent species and those listed on the product labels, despite high product authenticity. We suggest the false positives result from the sensitivity of DNA metabarcoding and filtering thresholds should be integrated into protocols to reduce false positives. Our results highlight how integrating DNA metabarcoding into the toolbox of analytical methods for quality control of fresh and/or processed plant-based food can improve product quality.

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Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products

Cited by 46 publications

References 40 publications

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika

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