Food Targeting: Determination of the Cocoa Shell Content (Theobroma cacao L.) in Cocoa Products by LC-QqQ-MS/MS

Abstract

A targeted metabolomics LC-ESI-QqQ-MS/MS application for the determination of cocoa shell based on 15 non-polar key metabolites was developed, validated according to recognized guidelines, and used to predict the cocoa shell content in various cocoa products. For the cocoa shell prediction, different PLSR models based on different cocoa shell calibration series were developed and their suitability and prediction quality were compared. By analysing samples from different origins and harvest years with known shell content, the prediction model could be confirmed. The predicted shell content could be verified with a deviation of about 1% cocoa shell. The presented method demonstrates the suitability of the targeted application of metabolomic profiling for the determination of cocoa shell and its applicability in routine analysis is discussed.

The effect addition of palm oil and coconut oil on the proximate value of pendawa chocolate, North Sumatra

“…The produced chocolate bars were analyzed for water content using the drying method (modified) [6], ash content using the dry ash method [7], fat content of the chocolate bars using the soxhlet method (limited) [8], and protein content using kjedhal method [9].…”

Kajian Penerimaan Panelis Terhadap Produk Coklat Batangan Pendawa, Sibiru-Biru, Deli Serdang, Sumatra Utara

“…Kemudian dilakukan penyangraian terhadap biji kakao kering menggunakan mesin roasting dengan suhu 90 o C kurang lebih selama 60 menit. Selanjutnya pada dilakukan proses pemisahan kulit kakao dengan menjadi Nib dengan menggunakan mesin NSS (Nib Shell Separator) (Cain et al, 2020). Setelah itu, dilakukan proses pemastaan biji kakao kering dan dilanjutkan dengan proses pengempaan.…”

Discrepancy Study of the Chemical Constituents of Panax Ginseng from Different Growth Environments with UPLC-MS-Based Metabolomics Strategy

“…Benefiting from modern technology, high-resolution mass spectrometry, such as Quadrupole Time of Flight (Q-TOF), has been maturely applied to the non-targeted study of metabolites without reference [ 25 , 26 , 27 , 28 , 29 ]. In addition, liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI-QQQ-MS/MS) coupled with multiple reaction monitoring (MRM) is also a mature technique for marker-targeted quantitative analysis [ 30 , 31 ]. However, there are few overall studies on targeting and non-targeting of metabolites at the same time, resulting in the inability to fully reveal the characteristics of a particular medicinal material.…”