Food safety using NMR-based metabolomics: Assessment of the Atlantic bluefin tuna, Thunnus thynnus, from the Mediterranean Sea

Cappello, Tiziana; Giannetto, Alessia; Parrino, Vincenzo; Marco, Giuseppe De; Mauceri, Angela; Maisano, Maria

doi:10.1016/j.fct.2018.03.038

Cited by 61 publications

(34 citation statements)

References 37 publications

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“…Xenobiotics lead to variations in cellular or biochemical components, processes, structures or functions ("biomarkers") in sentinel organisms ("bioindicators") (Shugart et al 2003). Different strategies have been applied using a number of biomarkers in different bioindicators to evaluate the pollutantinduced stress syndrome in aquatic environments (Viarengo et al 2007;Cappello et al 2018Cappello et al , 2019Gornati et al 2019;Caricato et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Attaallah

Marchionni

El-Beltagy

et al. 2020

The European Zoological Journal

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A huge progress has been achieved in mammalian in vitro technique. Instead, of the many trials to develop marine invertebrate cell cultures, only a few have obtained them and only from few tissues. Since in vitro cell culture of invertebrates could be very useful for many aspects of basic and applied science, in this work we investigate and describe the development of a technique for the establishment of cell cultures from gill, mantle and gonadic tissue of the Manila clam (Ruditapes philippinarum). We maintained viable cultures for up to 25 days. Culture viability and proliferation were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and with trypan blue, while an antibody against the ATP-dependent RNA helicase VASA, a protein expressed in the germline, and in multipotent stem cells of some animals, was used to verify the presence of these cell types. Following the described protocol: 1) explant resulted the better source to obtain cell cultures, when compared to enzymatic dissociation; 2) cultures of suspended cells were viable for longer period than adherent cells; 3) cell cultures obtained from tissues sampled in September-October performed better compared to other periods of the year, regarding maintenance and growth; 4) the tissue from which we obtained longer-lived cell cultures was gonadic tissue, especially form samples that show more undifferentiated germ cells and more VASA-stained cells. This study describes the challenges concerning the development of in vitro culture techniques for aquatic invertebrates.

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Section: Introductionmentioning

confidence: 99%

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Attaallah

Marchionni

El-Beltagy

et al. 2020

The European Zoological Journal

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“…Metabolomics can be used to analyze changes in all metabolites in organisms caused by changes in nutritional status [ 8 , 9 ], and therefore it can directly reveal the chemical processes and metabolic state changes in organisms. It is also useful in exploring the metabolic and physiological status of an organisms from the metabolites [ 10 , 11 ]. Metabolic balances may be disturbed by many factors, such as dietary composition [ 12 ] and nutrients [ 13 ] have been shown to influence metabolism.…”

Section: Introductionmentioning

confidence: 99%

Metabonomic Responses of Grazing Yak to Different Concentrate Supplementations in Cold Season

Zhou

Yue

Peng

et al. 2020

Animals

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Supplementation plays an important role in reversing the weight loss of grazing yaks during cold season. However, little is known about the effect of supplementation on the serum metabolites of grazing yaks. The objective of this study was to explore the effects of supplementary feeding on average daily gain (ADG) and serum metabolites with nuclear magnetic resonance (NMR)-based metabolomics method in growing yaks during cold season on the Qinghai-Tibetan plateau. Twenty 1.5-year-old female yaks (91.38 ± 10.43 kg LW) were evenly divided into three treatment groups and a control group (CON) (n = 5 per group). All the yaks were released to graze during daytime, whereas the yaks in the treatment groups were supplemented with highland barley (HLB), rapeseed meal (RSM), and highland barley plus rapeseed meal (HLB + RSM) at night. The whole experiment lasted for 120 days. Results indicated that the ADG of growing yak heifers was increased by concentrate supplementations, and ADG under HLB and HLB + RSM group was 37.5% higher (p < 0.05) than that with RSM supplementation. Supplementary feeding increased the plasma concentrations of total protein (TP), albumin (ALB), and blood urea nitrogen (BUN) of those in the CON group, and concentrations of BUN were higher in the RSM group than in the HLB and HLB + RSM group. Compared with the CON group, serum levels of glutamine, glycine, β-glucose were lower and that of choline was higher in the HLB group; serum levels of lactate were lower and that of choline, glutamate were higher in the HLB + RSM group. Compared with the HLB + RSM group, serum levels of glycerophosphoryl choline (GPC) and lactate were higher, and those of choline, glutamine, glutamate, leucine, N-acetyaspartate, α-glucose, and β-glucose were lower in the HLB group; serum levels of citrate, GPC and lactate were higher, and those of 3-Hydroxybutyrate, betaine, choline, glutamate, glutamine, N-acetylglycoprotein, N-acetyaspartate, α-glucose, and β-glucose were lower in the RSM group. It could be concluded that concentrate supplementations significantly improved the growth performance of growing yaks and supplementation with HBL or HLB plus RSM was better than RSM during the cold season. Supplementation with HBL or HLB plus RSM affected the serum metabolites of grazing yaks, and both treatments promoted lipid synthesis. Supplementation of yaks with HBL plus RSM could improve energy-supply efficiency, protein and lipid deposition compared with HLB and RSM.

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“…Cyclooxygenase-2 (COX-2) is a protein encoded by mitochondria, an important component of the oxidative phosphorylation pathway, which plays an important role in the formation of brown adipocytes (Aguirre et al 2017). Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) is a coenzyme factor of PPARγ (Puigserver 1998;Cappello et al 2018) which can increase energy consumption of BAT and is a major regulator of transcription factors involved in cell metabolism (Chen et al 2016). Previous studies have shown that plateau pika (Ochotona curzoniae) increase thermogenesis by browning in subcutaneous WAT and increasing BAT mass to adapt to cold temperatures, and the expressions of UCP1 and PCG-1a are upregulated in this process (Li et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

Zhang

Zhu

et al. 2019

The European Zoological Journal

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Animals employ various strategies to adapt to different natural habitats. In order to investigate the relationship between thermogenesis of adipose tissue and seasonal variations in Tupaia belangeri, body mass, food intake, adipose tissue morphology, and expression levels of three adipose differentiation-related genesperoxisome proliferators-activated receptor α (PPARα), cyclooxygenase-2 (COX-2) and peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α)were measured in the present study. The results show that body mass and food intake increased significantly in winter compared with summer. White adipose tissue (WAT) in winter demonstrated the characteristic appearance of beige adipocytes, including smaller and multi-chamber lipid droplets, darker hue, and increased expression levels of COX-2 and PGC-1α genes, which would increase thermogenic properties in T. belangeri to make up for the lack of thermogenesis in nonshivering thermogenesis (NST) caused by brown adipose tissue (BAT). Moreover, analysis of BAT morphology showed smaller lipid droplets, and COX-2 and PPARα gene expression in BAT increased significantly in winter, indicating that uncoupling protein 1 (UCP1) had been activated to increase NST. All of these results suggest that T. belangeri enhanced thermogenic capacity in winter, including WAT undergoing "browning" and enhanced NST in BAT, which played important roles in the strategies of thermal physiology and bioenergetics in T. belangeri during seasonal change.

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Food safety using NMR-based metabolomics: Assessment of the Atlantic bluefin tuna, Thunnus thynnus, from the Mediterranean Sea

Cited by 61 publications

References 37 publications

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Metabonomic Responses of Grazing Yak to Different Concentrate Supplementations in Cold Season

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

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