N-ACETYLGALACTOSAMINE BIOSENSOR BASED ON POLYDIACETYLENE NANOVESICLES: AN INDIRECTLY COLORIMETRIC RECOGNITION OF GALACTOSE-DEFICIENT IgA1

Abstract

Immunoglobulin A nephropathy (IgAN) has been recognized as the most prevalent form of glomerulonephritis with various histologic and clinical phenotypes in the world. The Gal-de¯cient IgA1 with terminally exposed GalNAc residue(s) plays a key role in the pathogenesis of the disease. In this study, a novel colorimetric biosensor based on Helix aspersa agglutinin (HAA) absorbed on polydiacetylene (PCDA) nanovesicles for N-acetylgalactosamine (GalNAC) molecular as core framework of Gal-de¯cient IgA1 recognition was investigated using the high a±nity between lectin HAA and GalNAC. The PCDA nanovesicles were prepared via UV crosslinking. The size, morphology and elastic property of the nanovesicles were tested. The optimal concentration of HAA for the recognition of GalNAC via naked eyes observation was 3 mM and the critical concentration of GalNAC for the sensitive color transition was 2 g/mL. This novel method has many advantages such as low prices, intuitive, real-time and easy to carry out and great potential in Gal-de¯cient IgA1 detection.

Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes

“…The GalNAc biosensor based on poly(diacetylene) nanovesicles developed by Hao et al. was applied for a noninvasive and real‐time colorimetric analysis of galactose‐deficient IgA1 (playing an important role in the pathogenesis of glomerulonephritis—IgA nephropathy) using nanovesicles modified with Helix aspersa agglutinin for naked‐eye detection …”