Following-up skin penetration of lidocaine from different vehicles by Raman spectroscopic mapping

Bakonyi, Mónika; Gácsi, Attila; Kovács, Anita; Szűcs, Mária-Budai; Berkó, Szilvia; Csányi, Erzsébet

doi:10.1016/j.jpba.2018.02.056

Cited by 27 publications

(20 citation statements)

References 26 publications

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“…The Raman correlation map proves the presence of the penetrated drug formulations in the different regions of the human skin, from the skin surface to the lower layers of the dermis after the treatment with the different compositions. The Raman spectra of the skin are really diverse and consist of numerous bands originating from different skin segments (e.g., nucleic acids, lipids, proteins) [27,31,32]. Several bands are overlapping with the spectra of the examined preparations.…”

Section: Raman Mappingmentioning

confidence: 99%

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Novel In Vitro Investigational Methods for Modeling Skin Permeation: Skin PAMPA, Raman Mapping

et al. 2020

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The human skin is marked as a standard by the regulatory agencies in the permeation study of dermal formulations. Artificial membranes can substitute human skin to some extent. Academicians and pharmaceutical corporations are focusing their efforts on developing standardized protocols and safe, reliable options to substitute human skin for carrying out permeability studies. Our research aim was to study the applicability of new techniques in the case of different types of dermal formulations. The skin parallel artificial membrane permeability assay (PAMPA) method and Raman mapping were compared to the gold-standard Franz cell method. A hydrogel and two types of creams were investigated as the most generally used dermal preparations. The values of the diffused drug were closer to each other in PAMPA and Franz cell measurement. The diffused amount of drug showed the same order for the different formulations. These results correlate well with the results of Raman mapping. Our conclusions suggest that all early screening examinations can be performed with model tools such as skin PAMPA supplemented with methods like Raman mapping as a semi-quantitative method.

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Section: Raman Mappingmentioning

confidence: 99%

“…Raman spectroscopy can give information about the spatial distribution of the drug in the skin layers based on the characteristic vibrational energy levels of the structure of a molecule. This semi-quantitative technique is suitable for monitoring the permeation of exogenous materials into the skin layers [24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Novel In Vitro Investigational Methods for Modeling Skin Permeation: Skin PAMPA, Raman Mapping

et al. 2020

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“…Therefore, this method is promising for discovering modifications in the structure of skin components. In addition, it is good for following up the penetration/permeation of APIs [114,115]. Nowadays, Raman microscopy is a powerful technique to properly understand skin structure and percutaneous drug delivery [111,[116][117][118].…”

Section: Confocal Raman Microscopic Methodsmentioning

confidence: 99%

Methods to Evaluate Skin Penetration In Vitro

et al. 2019

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Dermal and transdermal drug therapy is increasing in importance nowadays in drug development. To completely utilize the potential of this administration route, it is necessary to optimize the drug release and skin penetration measurements. This review covers the most well-known and up-to-date methods for evaluating the cutaneous penetration of drugs in vitro as a supporting tool for pharmaceutical research scientists in the early stage of drug development. The aim of this article is to present various experimental models used in dermal/transdermal research and summarize the novel knowledge about the main in vitro methods available to study skin penetration. These techniques are: Diffusion cell, skin-PAMPA, tape stripping, two-photon microscopy, confocal laser scanning microscopy, and confocal Raman microscopic method.

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“…Raman spectroscopic measurements were performed on a DXR2xi Raman imaging microscope equipped with a charge‐coupled device camera and a semiconductor laser. Each spectrum of the sample was collected at a laser excitation wavelength of 785 nm and power of 30 mW, using 1000 scans, an exposure time of 0.2 seconds and a slit width of 50 µm, 30,51 including fluorescence corrections.…”

Section: Methodsmentioning

confidence: 99%

A study of the diffusion behaviour of reactive dyes in cellulose fibres using confocal Raman microscopy

Yang

Dai

Zhong

et al. 2020

Coloration Technology

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Confocal Raman microscopy was used as a non‐destructive tool for in situ investigation of the diffusion of CI Reactive Blue 194 in viscose fibres. Dyed fibres were analysed by Raman depth imaging along the radius at different dyeing times (30, 60, 90, 120, 150, 180, 240, 300, 600, 900, 1200, 1500, 1800, 2100 and 2400 seconds) and temperatures (30, 40, 50, 60, 70 and 80°C). The dye concentration in the fibre was expressed by normalising the characteristic peak of dyes at 1577 cm−1 to eliminate the interference of the fibre. The Raman depth mapping images showed that dye molecules gradually diffused to the interior of the fibre with an increase in dyeing time, remaining ring‐shaped in the state of diffusion. In addition, the diffusion rate increased with increases in the dyeing temperature. This was understandable because dye molecules with a higher kinetic energy move faster. It was also found that the external structure of fibres played a significant role in the diffusion behaviour of CI Reactive Blue 194 during the initial stage of dyeing. The concentration gradient distribution of CI Reactive Blue 194 in the fibre was reflected by Raman depth mapping. The study demonstrates the potential of confocal Raman microscopy for monitoring the dynamic diffusion of dye molecules in fibres.

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Following-up skin penetration of lidocaine from different vehicles by Raman spectroscopic mapping

Cited by 27 publications

References 26 publications

Novel In Vitro Investigational Methods for Modeling Skin Permeation: Skin PAMPA, Raman Mapping

Novel In Vitro Investigational Methods for Modeling Skin Permeation: Skin PAMPA, Raman Mapping

Methods to Evaluate Skin Penetration In Vitro

A study of the diffusion behaviour of reactive dyes in cellulose fibres using confocal Raman microscopy

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