Following-up hemorheological consequences of gonadectomy in male and female rats

Abstract

Growing number of clinical and experimental data reflect to the gender differences of hemorheological parameters. However, little is known about the potential hemorheological effect of gonadectomy and consequent changes in sex hormone concentration. Adult, sameaged male and female rats were involved in the study. In control male and female group no surgical intervention was performed. In gonadectomized (GoE) male and female groups bilateral orchidectomy or ovariectomy were completed. Body weight measurement and blood sampling were carried out in the 1 st , 2 nd and 3 rd postoperative months. The GoE females had significant bodyweight augmentation and their plasma estrogen concentration decreased by 40-45% by the 1 st postoperative month, while in males the testosterone level was not detectable after gonadectomy. Leukocyte and platelet counts moderately increased in GoE males. Elongation index values of erythrocytes slightly decreased in both genders after gonadectomy, showing converging values. Erythrocyte aggregation index values of GoE females significantly raised by the 2 nd month. It can be concluded that gonadectomy in rats resulted in alteration (dominantly impairment) of blood micro-rheological parameters, by different manner in males and females. Supposedly decrease in estrogen can cause more expressed hemorheological changes than the cessation of testosterone.

Hemorheological consequences of hind limb ischemia-reperfusion differs in normal and gonadectomized male and female rats

“…Growing number of data underline the importance of hemorheological gender differences not only in the clinical investigations but also in the experimental medicine [32]. Furthermore, gonadectomy may additionally influence these micro-rheological gender differences, reflecting the influence of sex hormones, too [33]. The protective effect of estrogen has been demonstrated in numerous I/R studies including acute [47] and chronic hind limb [23], myocardial [10,27,30,37,52], brain [15,41] and intestinal ischemia [48].…”

“…The general anesthesia was provided by intraperitoneal administration of Thiopenthal ® (60 mg/kg). [33] the same protocol was completed as in I/R groups.…”

“…Concerning the hemorheological gender differences in rats [32] and their changes after gonadectomy [33], it is supposed that the effect of I/R on erythrocyte deformability and aggregation may alter in female and males, that might differ further in gonadectomized animals. We aimed to investigate this question in a hind limb I/R follow-up study in rats.…”

Estradiol Valerate Affects Hematological and Hemorheological Parameters in Rats

“…The hematological and hemorheological parameters show differences depending on gender, hormonal status, and menstrual cycle [ 8 , 9 , 10 ]. Guillet et al found that during menstruation, the red blood cell deformability is decreased in the pre-ovulation and post-ovulation phases [ 9 ].…”

Age- and gender-related hemorheological alterations in intestinal ischemia-reperfusion in the rat

“…In addition, only a few studies compared the gender-related responses of microrheological parameters in I/R injuries. [14][15][16][17] The aim of our study was to investigate the effects of intestinal I/R on systemic hemorheological parameters in a rat model. We hypothesized that during intestinal ischemia, the microrheological parameters of the blood worsen, and the degree of these changes differs in male and female, young and older rats.…”