Estradiol Valerate Affects Hematological and Hemorheological Parameters in Rats

Abstract

Polycystic ovary syndrome (PCOS) is one of the most common endocrinological diseases in women. Although the risk of cardiovascular diseases is high in PCOS, the number of scientific publications describing hemorheological changes is not significant. We aimed to perform a comprehensive hematological and micro-rheological study on experimentally induced PCOS in rats.Wistar rats were divided into control (n = 9) and PCOS groups (n = 9), in which animals received single-dose estradiol valerate. Measurements were carried out before treatment and monthly for four months. Bodyweight, blood glucose concentration, hematological parameters, red blood cell (RBC) deformability, and aggregation were measured. A histological examination of the ovary was performed at the end of the experiment. The blood glucose level and the bodyweight were significantly elevated vs. base in the PCOS group. A significant decrease was seen in RBC count, hemoglobin, and hematocrit. The maximal elongation index showed a significant increase. PCOS also resulted in a significant increase in RBC aggregation index parameters. The histological and hormone examinations confirmed developed PCOS. The administration of estradiol valerate caused significant changes during the examined period in hematological and hemorheological parameters. Our results draw attention to the possible usefulness of micro-rheological investigations in further studies on PCOS.

High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats

“…Literature data showed numerous protocols for PCOS induction in rodents where scientists tried to resemble its complex and multifaceted appearance. Regarding the EV protocol applied alone, there has been a spectrum of different results observed, from those that suggest that administration of EV is not appropriate to establish metabolic profile with impaired glycoregulation similar to PCOS, according to standard biochemical techniques for glucose measurements [ 39 , 66 ], to those that showed elevated fasting blood glucose and impaired glycoregulation with insulin resistance [ 54 , 67 ]. In addition, Danesa et al found elevated fasting blood glucose in the PCOS group, but without difference after OGTT between the PCOS and control group [ 68 ].…”