Following-up changes in red blood cell deformability and membrane stability in the presence of PTFE graft implanted into the femoral artery in a canine model

Effects and influencing factors on hemorheological variables taken into consideration in surgical pathophysiology research

“…Mechanical trauma to blood cells also has to be taken into consideration [5,19,20]. In conditions leading to ischemia and reperfusion (e.g., clamping vessels, obturation/occlusion and revascularization) and by usage of various intravascular devices for diagnosis and for therapy (including vascular stents, grafts, prostheses, artificial valves, etc), mechanical trauma to blood appears [5,7]. For instance, in sepsis, red blood cells can be damaged mechanically due to the presence of thrombi and micro-thrombi (DIC), or even during therapeutic efforts, such as direct removal of toxins from the bloodstream by high-volume hemofiltration as well [5,21].…”

“…In surgical pathophysiology ischemia-reperfusion -as part of necessary interventions in parenchymal organ surgery, operations on extremities, tissue/organ transplantation, presented in compartment syndromes, traumatic vessel occlusions-, inflammatory processes, sepsis, vascular interventions, such as intravascular devices, presence of anastomoses, shunts, tissue trauma, shock, all mean conditions in which hemorheological parameters show alterations [3][4][5][6][7]. The magnitude of changes can be different, though, dominantly being non-specific.…”

Influence of stent implantation on erythrocyte aggregation in human native coronary arteries and saphenous vein grafts

“…45,46 Long-term exposure to exogenous devices is associated with increased RBC aggregation. 47,48 Here, stent implantation did not induce hemolysis, possi- 53,54 In the present study, the increased RBC aggregation in coronary aspirate was independent of the treated vessel (RCA or SVG-RCA) and therefore of the amount of released particulate debris. The ex vivo supplementation of pure RBCs from healthy volunteers with patient aspirate plasma increases RBC aggregation by the same order of magnitude as the aspirated plasma during stent implantation.…”

“…Thus, neither the location of blood withdrawal nor the different withdrawal techniques and the resulting different shear and tensile stresses during blood sampling (venous blood samples from the cubital vein with a butterfly cannula; arterial blood samples from coronary arteries taken with an aspiration catheter) had impact on the determined RBC aggregation in vitro and under static conditions. Mechanical stress: Implantation of exogenous devices (eg, circulatory support devices, polytetrafluoroethylene grafts) induces mechanical stress on blood cells, resulting in mechanical instability of the RBC membrane and hemolysis . Long‐term exposure to exogenous devices is associated with increased RBC aggregation . Here, stent implantation did not induce hemolysis, possibly because of only short‐term exposure of RBC to the stent. Contrast agent: Physical components, that is, plasma viscosity, influence RBC aggregation .…”

Cinética de lise mecânica de eritrócitos humanos e sua associação com variáveis bioquímicas, hematológicas e oxidativas em indivíduos saudáveis e em indivíduos com diabetes mellitus tipo 1

“…In erythrocytes, stability and deformability are properties that depend on a wide range of factors, such as the composition of the membrane and the complex interactions between membrane proteins and cytoskeleton (Discher, 2000;Popescu, Park, Dasari, Badizadegan, & Feld, 2007;Sun et al, 2007), the magnitude of the surface the cell relative to the volume, cell morphology, hemoglobin concentration and intracellular viscosity, among others (Reinhart, Piety, Goede, & Shevkoplyas, 2015;Stoltz, Singh, & Riha, 1999;Toth et al, 2014;Zhan, Loufakis, Bao, & Lu, 2012).…”