Follow-up study of morphology and cardiac function in rats undergoing induction of supravalvular aortic stenosis

Ribeiro, Henrique Barbosa; Okoshi, Katashi; Cicogna, Antônio Carlos; Bregagnollo, Edson Antônio; Rodrigues, Maria Aparecida Marchesan; Padovani, Carlos Roberto; Aragon, Flávio Ferrari; Jamas, Elenize; Okoshi, Marina Politi

doi:10.1590/s0066-782x2003001400003

Cited by 14 publications

(20 citation statements)

References 35 publications

(29 reference statements)

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“…The increase of left ventricle wall relative thickness in stenosis group indicates concentric hypertrophy. The data are in agreement with previous studies that showed cardiac remodeling in this experimental model 5,9,11,20 . Remodeling of the left chamber is an adaptive response to ventricular pressure rise; the increase in wall thickness and cavity volume decrease normalize parietal stress and, in consequence, LV function 1,21 .…”

Section: Discussionsupporting

confidence: 93%

“…The ΔD endo observed at week 6 was similar to that reported by Bregagnollo et al 7,22 , Ribeiro et al 11 and Litwin et al 9 who found an improved systolic function at this time. Furthermore, these authors also observed worsening after 18 or 21 weeks.…”

Section: Discussionsupporting

confidence: 88%

“…In this model, animals develop cardiac remodeling that is associated, in the short term, with diastolic dysfunction and improved systolic function followed by depressed systolic performance and heart failure 10,11 .…”

mentioning

confidence: 99%

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Tolerância ao esforço em ratos com estenose aórtica e disfunção ventricular diastólica e/ou sistólica

Mendes

Sugizaki

Campos

et al. 2013

Arq. Bras. Cardiol.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

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Tolerância ao esforço em ratos com estenose aórtica e disfunção ventricular diastólica e/ou sistólica

Mendes

Sugizaki

Campos

et al. 2013

Arq. Bras. Cardiol.

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“…At 10 -12 wk postbanding, the animals enter a decompensated stage with depressed LV developed pressure (LVDP) and dP/dt. In this model of LVH, no systemic activation of the sympathetic nervous system or of the renin-angiotensin-aldosterone system occurs (46). Consequently, there are no signs of cardiac lesions, peripheral arteritis, myocardial necrosis, or extensive fibrosis.…”

Section: Methodsmentioning

confidence: 91%

“…This banding procedure relies on the natural growth of the animal to produce a gradually increasing degree of aortic constriction. The rats develop a concentric hypertrophy and increased heart-to-body weight ratio, which is associated, in the short term, with improvement in the systolic function of the heart (27,42,46,48,60). At 10 -12 wk postbanding, the animals enter a decompensated stage with depressed LV developed pressure (LVDP) and dP/dt.…”

Section: Methodsmentioning

confidence: 99%

Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo

O’Donnell

Fields²,

Xu³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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O'Donnell JM, Fields A, Xu X, Chowdhury SA, Geenen DL, Bi J. Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo. Am J Physiol Heart Circ Physiol 295: H2483-H2494, 2008. First published October 24, 2008 doi:10.1152/ajpheart.01023.2008.-Adenoviral gene transfer of sarco(endo)plasmic reticulum Ca 2ϩ -ATPase (SERCA)2a to the hypertrophic heart in vivo has been consistently reported to lead to enhanced myocardial contractility. It is unknown if the faster skeletal muscle isoform, SERCA1, expressed in the whole heart in early failure, leads to similar improvements and whether metabolic requirements are maintained during an adrenergic challenge. In this study, Ad.cmv.SERCA1 was delivered in vivo to aortic banded and shamoperated Sprague-Dawley rat hearts. The total SERCA content increased 34%. At 48 -72 h posttransfer, echocardiograms were acquired, hearts were excised and retrograded perfused, and hemodynamics were measured parallel to NMR measures of the phosphocreatine (PCr)-to-ATP ratio (PCr/ATP) and energy substrate selection at basal and high workloads (isoproterenol). In the Langendorff mode, the rate-pressure product was enhanced 27% with SERCA1 in hypertrophic hearts and 10% in shams. The adrenergic response to isoproterenol was significantly potentiated in both groups with SERCA1.31 P NMR analysis of PCr/ATP revealed that the ratio remained low in the hypertrophic group with SERCA1 overexpression and was not further compromised with adrenergic challenge.13 C NMR analysis revealed fat and carbohydrate oxidation were unaffected at basal with SERCA1 expression; however, there was a shift from fats to carbohydrates at higher workloads with SERCA1 in both groups. Transport of NADH-reducing equivalents into the mitochondria via the ␣-ketoglutamate-malate transporter was not affected by either SERCA1 overexpression or adrenergic challenge in both groups. Echocardiograms revealed an important distinction between in vivo versus ex vivo data. In contrast to previous SERCA2a studies, the echocardiogram data revealed that SERCA1 expression compromised function (fractional shortening) in the hypertrophic group. Shams were unaffected. While our ex vivo findings support much of the earlier cardiomyocyte and transgenic data, the in vivo data challenge previous reports of improved cardiac function in heart failure models after SERCA intervention. sarco(endo)plasmic reticulum Ca 2ϩ -ATPase; glucose oxidation; fatty acid oxidation; phosphocreatine-to-ATP ratio; myocardial energy potential THE CHANGES in intracellular Ca 2ϩ handling reported for the failing myocardium have been linked to sarco(endo)plasmic reticulum Ca 2ϩ -ATPase (SERCA). Calcium uptake into the sarcoplasmic reticulum (SR) by SERCA determines the rate of calcium removal for relaxation, the SR calcium content, and calcium released for contractions (33,45). In the failing heart, it has been proposed that SERCA contributes to reduced contractil...

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Congestive heart failure model representing aortic banding induced hypertrophy: A study to analyse extent of pressure overload and alteration in myocardial structure and function

Patel

2021

IJC Heart & Vasculature

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Congestive Heart failure (CHF) is a severe pathology representing a major public health problem in industrialized nations which is increasing in prevalence and incidence. The aortic banding rat model provides steady progression of cardiac dysfunction under chronic pressure overload. Present study evaluated two abdominal aortic constriction techniques including constriction of aorta above renal arteries and between renal arteries. The extent of constriction was varied with 22 G and 24 G needles and the duration for evaluation of CHF was also varied by terminating the banded animals after 6 and 8 weeks of banding. Various hemodynamic, ECG and tissue parameters were evaluated after banding to see the progression of CHF. The findings revealed that the constriction of the aorta above both renal arteries with 24 G needle is a better technique amongst other tested banding techniques as the rate of progression of CHF was found to be maximum with it. On the basis of above study, it was concluded that, aortic banding above both renal arteries with 24 G needle is a better technique for induction of pressure overload and for further observation in transition of the cardiac compensatory to decompensatory phase, the duration of the model needs to be prolonged.

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Follow-up study of morphology and cardiac function in rats undergoing induction of supravalvular aortic stenosis

Cited by 14 publications

References 35 publications

Tolerância ao esforço em ratos com estenose aórtica e disfunção ventricular diastólica e/ou sistólica

Tolerância ao esforço em ratos com estenose aórtica e disfunção ventricular diastólica e/ou sistólica

Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo

Congestive heart failure model representing aortic banding induced hypertrophy: A study to analyse extent of pressure overload and alteration in myocardial structure and function

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