Follow-Up of Natural Product Isolation

Cannell, Richard J. P.

doi:10.1007/978-1-59259-256-2_15

Cited by 23 publications

(26 citation statements)

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“…Our results revealed that the methanol roots extract had more toxicity than roots aqueous extract since the methanol had high polarity which could dissolve both polar and non polar components which then can be actively passed through the plasma membrane. Cannell (1998) and Rajendran and Ramakrishnan (2009) found that the high polarity of methanol extract of Artocarpus heterophyllus was responsible for inhibiting nearly 100% of Hep2 cells. The other explanation for the differences in cytotoxic activity between aqueous and methanol extracts may be due to the presence of the calystegin alkaloid extracted by methanol (Russel, 1993).…”

Section: Cytotoxic Effect Of (Aqueous and Methanol) Crude Extracts Pmentioning

confidence: 99%

Cytotoxic and cytogenetic effects of Convolvulus arvensis extracts on rhabdomyosarcoma (RD) tumor cell line in vitro

Asaad¹,

Dhamia²,

Kawthar³

2014

J. Med. Plants Res.

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The present study was designed to investigate the cytotoxicity of (aqueous and methanol) crude extracts of the leaves, stems and roots extracts as well as proteoglycan and glycoside fraction I (FI) of Convolvulus arvensis against human Rhabdomyosarcoma (RD) tumor cell line in vitro. The effect of glycoside FI fraction on mitotic index (MI) of RD cell line was investigated as well. The optical density (OD) of cell growth was measured by Elisa reader at 492 nm using tetrazolium bromide (MTT). Aqueous and methanol leaves extracts and glycoside FI had more cytotoxic effects at 10 mg/ml after 24 h. After 48 h, proteoglycan and glycoside FI at 10 mg/ml revealed very high cytotoxic activity compared with other concentrations. After 72 h, glycoside FI at 10 mg/ml had more cytotoxic inhibition compared with other extracts. Glycoside FI had cytotoxicity concentration 50% (CC 50%) 1.775, 0.870 and 0.706 mg/ml after 24, 48, and 72 h, respectively. The root aqueous extract had less cytotoxic effect after 72 h than other extracts; the CC 50% was 7.437 mg/ml. Cytotoxicity of root aqueous extract was more pronounced at higher concentration of 10 mg/ml. The effect of glycoside FI on MI of RD tumor cell line was concentration dependant.

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Section: Cytotoxic Effect Of (Aqueous and Methanol) Crude Extracts Pmentioning

confidence: 99%

Cytotoxic and cytogenetic effects of Convolvulus arvensis extracts on rhabdomyosarcoma (RD) tumor cell line in vitro

Asaad¹,

Dhamia²,

Kawthar³

2014

J. Med. Plants Res.

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“…The resultant fractions that give positive ferric chloride test 1% solution. Since the grape skin contains numerous amounts of chemical compounds, the extract cleaning up was necessary by liquid-liquid partition technique for separation compounds according to the different distribution coefficients and the solvent affinity to solutes 16 . Ethyl acetate is a good solvent for resveratrol taking up as viewed in many studies 17 .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of New Derivatives of Resveratrol and to Explore its Potential Health Benefits

Al-Jumaily¹,

Shafiq²,

Al-Bayati³

2012

Chem Sci Trans

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“…HPLC is a versatile, robust, and widely used technique for the isolation of chemical products. [21] Currently, this technique is gaining popularity among various analytical techniques as the main choice for fingerprinting study. [22] To understand the purpose of HPLC analytical method, it is necessary to consider the applications of HPLC in pharmaceutical analysis.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Logoyda¹

2018

IJGP

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Introduction: Modern methods of isolating drugs from biological material are based on the individual physic-chemical properties of the compounds, so the choice of optimal conditions for isolation from biological objects, cleaning extracts of impurities are a pressing issue for improving existing and developing new methods of analytical and bioanalytical analysis. The objective of this research was to select the optimal conditions for the extraction of enalapril maleate (EM) by organic solvents from water solutions in dependence on pH solutions. Materials and Methods: The chromatographic analysis of enalapril performed on liquid chromatograph ACQUITY Arc System. Results: The extraction of enalapril by organic solvents from water solutions in dependence on pH solutions has been conducted. The quantitative determination of enalapril by high-performance liquid chromatography methods has been conducted. Conclusion: As a result of studies, we have found that the optimal extragent is chloroform, which is extracted at pH 5-90.74% and methylene chloride is extracted at pH 4-81.39%. We have found that EM least extracted with hexane, so these conditions may be cleaned extract from coextractives impurities.

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Follow-Up of Natural Product Isolation

Cited by 23 publications

References 46 publications

Cytotoxic and cytogenetic effects of Convolvulus arvensis extracts on rhabdomyosarcoma (RD) tumor cell line in vitro

Cytotoxic and cytogenetic effects of Convolvulus arvensis extracts on rhabdomyosarcoma (RD) tumor cell line in vitro

Synthesis of New Derivatives of Resveratrol and to Explore its Potential Health Benefits

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