Follicular fluid VEGF levels directly correlate with perifollicular blood flow in normoresponder patients undergoing IVF

Monteleone, P.; Artini, Paolo Giovanni; Simi, Giovanna; Casarosa, Elena; Cela, Vito; Genazzani, Andrea Riccardo

doi:10.1007/s10815-008-9218-1

Cited by 53 publications

(39 citation statements)

References 16 publications

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“…An adequate blood supply is fundamentally important for the regulation of intrafollicular oxygen levels, nutrients, and determination of oocyte quality [40]. In view of this, various angiogenic factors have been investigated in follicular fluid and their effects on IVF outcome documented.…”

Section: Discussionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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Purpose The study aimed to investigate key intrafollicular prognostic factors among various cytokines and angiogenic molecules for prediction of mature oocytes and good-quality embryos in women with endometriosis undergoing in vitro fertilization (IVF). Methods Paired follicular fluid and serum samples were collected from 200 women with advanced stage endometriosis and 140 normal ovulating women during oocyte retrieval. The concentrations of cytokines (pro-inflammatory: IL-1β, TNF-α, IL-2, IL-8, IL-12, IFN-γ; anti-inflammatory: IL-4, IL-6, IL-10) and angiogenic molecules (vascular endothelial growth factor (VEGF), adrenomedullin, angiogenin) were determined in follicular fluid and serum using ELISA. Expression of these molecules was subjected to multivariate analysis for the identification of major predictive markers of oocyte and embryo quality. Receiver operating characteristic (ROC) curve was applied to determine the best cutoff point for the discrimination between mature and immature oocytes in these women. Results Significant increases in levels of cytokines and angiogenic molecules were observed in women with endometriosis compared to controls (P < 0.001). From the validated partial least squares-discriminant analysis (PLS-DA) model, IL-8, IL-12, and adrenomedullin were identified as the most important factors contributing to endometriosis and were negatively associated with oocyte maturity and embryo quality. Conclusion The levels of IL-8, IL-12, and adrenomedullin may be good indicators of embryo and oocyte quality in endometriosis patients undergoing IVF. Further studies are necessary to ascertain the potential of these markers for oocyte and embryo developmental competence which may help improve the chances of a successful IVF in endometriosis patients.

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Section: Discussionmentioning

confidence: 99%

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Singh

Dutta

Chattopadhyay

et al. 2016

J Assist Reprod Genet

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“…Vascular endothelial growth factor plays an important role for the vascularization of the follicle, and thus the regulation of the intrafollicular oxygen level and FF levels of VEGF directly correlated with perifollicular blood flow in patients undergoing IVF (24). A significantly higher FF concentration of VEGF has been reported in oocytes failing to fertilize, and high FF VEGF levels also have been associated with poor embryo quality and low pregnancy rates in IVF (25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan

Westergaard

Mikkelsen

et al. 2011

Fertility and Sterility

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“…It has been suggested that the competence of an oocyte for maturation, fertilisation and embryo viability after fertilisation mainly depends on the follicle (Moor et al 1998, Webb et al 2007. Greater blood flow in the follicle is related to the greater dissolved oxygen in the follicular fluid (Van Blerkom et al 1997, Monteleone et al 2008. The vascular system carries nutrients and oxygen to the follicle.…”

Section: Discussionmentioning

confidence: 99%

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

Siddiqui¹,

Gastal²,

Gastal³

et al. 2009

Reproduction

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The effect of the extent of vascular perfusion of the wall of the preovulatory follicle on in vitro cleavage rate of the recovered oocyte and embryo development to >8 cells was studied in 52 heifers. Heifers received a luteolytic dose of prostaglandin F2α (PGF2α) when the largest follicle was ≥11 mm. An ovulation-inducing injection of GnRH was given 36 h later (hour 0), and collection of follicular fluid and the oocyte was done at hour 26. Vascular perfusion of the follicular wall was assessed by colour Doppler ultrasonography at hours 0 and 26. Each of the recovered oocytes (41/52; 79%) was mature (extruded polar body). Cleavage and embryo development were assessed at 48 h and 120 h respectively, after in vitro fertilisation (IVF). The percentage of cleaved oocytes and >8 cell embryos was 80% (31/39) and 55% (17/31) respectively. Vascular perfusion of the follicular wall was greater (lower pulsatility index; P<0.001) for follicles that produced cleaved versus non-cleaved oocytes and greater (P<0.04) for follicles that produced >8 cell versus ≤8 cell embryos. Percentage of follicular wall with Doppler signals of blood flow was greater (P<0.001) for >8 cell versus ≤8 cell embryos. Follicular-fluid concentration of free IGF1 was lower for cleaved oocytes (P<0.001) and >8 cell embryos (P<0.05), and oestradiol was lower (P<0.05) for >8 cell embryos. Results supported the hypothesis that greater vascular perfusion of the wall of the preovulatory follicle was positively associated with IVF and embryo development.

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Follicular fluid VEGF levels directly correlate with perifollicular blood flow in normoresponder patients undergoing IVF

Cited by 53 publications

References 16 publications

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Relationship of vascular perfusion of the wall of the preovulatory follicle to in vitro fertilisation and embryo development in heifers

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