Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Humaıdan, Peter; Westergaard, Lars; Mikkelsen, Anne Lis; Fukuda, Misao; Andersen, Claus Yding

doi:10.1016/j.fertnstert.2011.02.013

Cited by 45 publications

(22 citation statements)

References 32 publications

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“…Some of the researchers found lower levels of VEGF in the group of women triggered with GnRH agonist [24], [25] while others did not find a difference between the two groups [26].…”

Section: Discussionmentioning

confidence: 96%

GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells

et al. 2014

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ObjectiveTo investigate the mRNA expression of genes related to steroidogenesis and OHSS in granulosa cells (GCs) of patients triggered with GnRH agonist compared to patients triggered with hCG.DesignMural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.SettingsSingle center, case control study.Patient(s)24 women who were treated with GnRH agonist or hCG for triggering of ovulation.InterventionsGC collection.Main Outcome Measure(s)The expression of genes related to steroidogenesis and OHSS in mural GCsResultsThe fertilization rate was similar in the two groups. The mRNA expression of CYP19A1 (0.50 vs 1, arbitrary unit), CYP11A1 (0.6 vs. 1) and 3 beta hydroxysteroid-dehydrogenase (0.39 vs 1) was significantly lower in the GnRH group. The expression of VEGF (0.74 vs. 1) and inhibin β B (0.38 vs 1) was lower in the GnRH analog triggered group.ConclusionExpression of genes related to steroidogenesis is lower at the time of oocyte retrieval in patients triggered with GnRH agonist. The decreased expression of VEGF and inhibin β B in the GnRH agonist group can explain the mechanism of early OHSS prevention.

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“…Some of the researchers found lower levels of VEGF in the group of women triggered with GnRH agonist [24], [25] while others did not find a difference between the two groups [26].…”

Section: Discussionmentioning

confidence: 96%

GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells

et al. 2014

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“…Subsequent studies confirmed and extended these findings, with higher FF progesterone and amphiregulin levels seen after hCG trigger compared with GnRHa trigger [15,16]. Moreover, significant differences in early luteal endometrial gene-expression profiles were seen when comparing hCG trigger to GnRHa trigger [17].…”

Section: Discussionmentioning

confidence: 70%

Effect of GnRHa ovulation trigger dose on follicular fluid characteristics and granulosa cell gene expression profiles

Vuong

et al. 2017

J Assist Reprod Genet

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Purpose A recent dose-finding study showed no significant differences in number of mature oocytes, embryos and topquality embryos when triptorelin doses of 0.2, 0.3 or 0.4 mg were used to trigger final oocyte maturation in oocyte donors co-treated with a gonadotropin-releasing hormone (GnRH) antagonist. This analysis investigated whether triptorelin dosing for triggering final oocyte maturation in oocyte donors induced differences in follicular fluid (FF) hormone levels and granulosa cell gene expression. Methods This single-centre, randomised, parallel, investigatorblinded trial was conducted in oocyte donors undergoing a single stimulation cycle at IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam, from August 2014 to March 2015.A total of 165 women aged 18-35 years with body mass index <28 kg/m 2 , anti-Müllerian hormone >1.25 ng/mL, and antral follicle count ≥6 were randomised to three different triptorelin doses for trigger. The main outcome was concentration of steroid hormones in FF collected from the first punctured follicle on each side. Moreover, luteinising hormone receptor (LHR), 3β-hydroxy-steroid-dehydrogenase (3ßHSD) and inhibin-Ba (INHB-A) gene expression in cumulus and mural granulosa cells were investigated in a subset of women from each group. Results Progesterone and oestradiol levels in FF did not differ significantly by trigger doses; findings were similar for 3βHSD, LHR and INHB-A gene expression in both cumulus and mural granulosa cells. Conclusions In women co-treated with a GnRH antagonist, no significant differences in FF steroid levels and granulosa cell gene expression were seen when different triptorelin doses were used to trigger final oocyte maturation.

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“…Indeed, previous studies indicate that GnRHa trigger may offer significant advantages over HCG trigger, taking these variables into account (Bodri et al, 2010;Cerrillo et al, 2009;Hernandez et al, 2009;Humaidan et al, 2011;Oktay et al, 2010). Interestingly, one co-author of the present Cochrane analysis previously published results showing that the implantation potential of frozen-thawed embryos deriving from GnRHa trigger is similar to that of embryos deriving from HCG trigger (Griesinger et al, 2007).…”

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confidence: 72%

“…During recent years, however, there has been an increasing focus on the quality of some of the published Cochrane reviews and meta-analyses. Thus, several authors have pointed out that some meta-analyses are based on scant information, and, even worse, are conducted during the development of new concepts (Humaidan and Polyzos, 2012;Humaidan et al, 2011;.…”

mentioning

confidence: 99%

“…We also suggested that the meta-analysis was too premature because the number of trials was restricted and included a limited number of participants. Moreover, data were compiled from studies that were not comparable as different luteal phase protocols were used (Humaidan et al, 2011). Unfortunately, in the new Cochrane review, it seems that the authors repeat the same errors as in their initial review.…”

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confidence: 99%

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The updated Cochrane review 2014 on GnRH agonist trigger: repeating the same errors

Kol

Humaıdan

Alsbjerg

et al. 2015

Reproductive BioMedicine Online

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Cochrane reviews are powerful tools, internationally recognized as the highest standard in evidence-based health care. A Cochrane analysis makes use of precise, reproducible criteria in the selection of studies for review. In the context of a previous Cochrane review (2010) on the subject of gonadotrophin-releasing hormone agonist (GnRHa) trigger, we questioned whether a review should be conducted during the research phase when new concepts are being developed. Recently, an updated Cochrane review was published, reaching the same general conclusion as the first one, i.e., GnRHa triggers lower the chance of pregnancy in fresh autologous IVF and intracytoplasmic injection treatment cycles. We argue that the new review repeats previous errors by compiling data from studies that were not comparable as different luteal phase protocols were used. From the clinical point of view, the luteal support used is the variable which affects the pregnancy rate and not the use of the GnRHa trigger for final oocyte maturation. Therefore, a meaningful comparison between GnRHa and HCG trigger must be confined to outcome measures that are not affected by the luteal support used. We conclude that the updated review falls short of addressing meaningful clinical and fundamental questions in the context of GnRHa trigger.

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Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

Cited by 45 publications

References 32 publications

GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells

GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells

Effect of GnRHa ovulation trigger dose on follicular fluid characteristics and granulosa cell gene expression profiles

The updated Cochrane review 2014 on GnRH agonist trigger: repeating the same errors

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