Follicle survival and growth to antral stages in short-term murine ovarian cortical transplants after Cryologic solid surface vitrification or slow-rate freezing

Aerts, J.M.J.; Clercq, J. B. P. De; Andries, S.; Leroy, Jo; Aelst, Stefan Van; Bols, P.E.J.

doi:10.1016/j.cryobiol.2008.07.011

Cited by 31 publications

(25 citation statements)

References 46 publications

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“…Similar increases in follicular growth after cryopreservation and transplantation of ovine cortical tissue were observed [26]. Therefore, it is hypothesized that the ovary under physiological conditions is dominated by inhibitory mechanisms, which are no longer available during transplantation [14,26]. Our results also showed that VOAT mice had higher PCNA expression than intact mice.…”

Section: Discussionsupporting

confidence: 75%

“…It has been reported that PCNA-positive follicles after cryopreservation and 7-day autotransplantation were significantly increased, including the antral stage, in the ovarian tissues compared with control tissue [14]. Similar increases in follicular growth after cryopreservation and transplantation of ovine cortical tissue were observed [26].…”

Section: Discussionsupporting

confidence: 65%

“…Furthermore, assessment of follicle survival and growth in vitrified-warmed ovarian tissues at 7 days after autotransplantation to the kidney capsule revealed increased follicular proliferation compared to control [14]. However, it is still unclear whether vitrified-warmed ovarian tissue has the potential to support uterine preparation for implantation and subsequent pregnancy to full term after the autotransplantation.…”

Section: Introductionmentioning

confidence: 99%

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Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Matsumoto

Ezoe

Mitsui

et al. 2011

Reprod Medicine & Biology

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Purpose Our previous study demonstrated that heterotopic autotransplantation of fresh ovarian tissue followed by transfer of blastocysts supported full-term pregnancy in the mouse. In the present study, to address whether vitrified-warmed ovarian tissue has the potential to support uterine preparation for implantation and subsequent pregnancy to full term, we examined vitrified-warmed ovarian tissue autotransplantation (VOAT) in mice. Methods VOAT into kidney capsules was performed for sexual cycle-ceased mice after 7 days of ovariectomy. Uterine potential of decidualization was examined by oil infusion on day 4 of pseudopregnancy. Immunohistochemical analysis was performed to examine the potential in VOAT ovarian tissues. Blastocysts were transferred into uteri on day 4 of pseudopregnancy. Results In VOAT mice, uterine decidualization on day 8 of pseudopregnancy was the same as that in intact mice. Blastocyst transfer into the pseudopregnant VOAT mice showed the same rates of pregnancy and live birth pups as intact mice, while less steroidogenesis in the corpus luteum was detected in VOAT mice. Conclusions The autotransplantation of vitrified-warmed ovarian tissues after 7 days of ovariectomy restored their sexual cycle and then supported their pregnancy and production of offspring.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

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Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Matsumoto

Ezoe

Mitsui

et al. 2011

Reprod Medicine & Biology

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“…The cryologic solid surface vitrification method was found to be a simple and convenient freezing method for cryopreservation of ovarian tissue in this study, and has also been used successfully to cryopreserve embryos and oocytes with a high post-thaw survival rate in other species (Fry et al 2005, Beebe et al 2006, Costigan et al 2006. Aerts et al (2008) used the cryologic solid surface vitrification method to cryopreserve mouse ovarian tissue and assessed the survival of thawed tissues using histological examination and viability staining 7 days after heterotypic allografting in the kidney. Their results showed the fraction of intermediary and primary follicles significantly increased in both vitrified and slow-cooled grafts, but slow-cooled tissues yielded a significantly higher proportion of further developed follicles, including secondary and antral follicles, suggesting that the solid surface method may have inhibited follicle growth.…”

Section: Discussionmentioning

confidence: 99%

“…Using this method, tissue samples are normally placed in a very small volume of vitrification solution and cooled on a sterile metal surface to maximize the cooling rate, and minimize the potential for contamination of samples by LN 2 . Measures of survival of vitrified ovarian tissue after cryopreservation have included morphological examination (histology), viability assessment by viability staining or development in vitro or in vivo culture (Yeoman et al 2005, Santos et al 2007, Aerts et al 2008. There are, however, limited data on the effects on the function of ovarian tissue vitrified using solid surface vitrification systems.…”

Section: Introductionmentioning

confidence: 99%

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Wang¹,

Catt²,

Pangestu³

et al. 2009

Reproduction

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Ovarian tissue cryopreservation and transplantation can be used to preserve fertility for cancer patients. In this study, we assessed the viability and function of ovarian tissue from adult mice that was cryopreserved by solid surface vitrification or traditional slow-cooling using various in vitro and in vivo techniques, including allotransplantation, in vitro oocyte maturation, embryo culture in vitro, blastocyst cryopreservation, embryo transfer, and development. The importance of cumulus cells for oocyte maturation, fertilization, and embryo development was investigated. Graft recovery, follicle survival, and oocyte retrieval was similar in control, vitrified, and slow-cooled groups. High rates of oocyte maturation, cleavage, and blastocyst formation were achieved, with no significant differences between the control, vitrified or slow-cooled ovarian tissue grafts. The presence of cumulus cells was important for oocyte maturation, fertilization, and subsequent development. Cumulus-oocyte complexes with no surrounding cumulus cells (N-COCs) or with an incomplete layer (P-COCs) had significantly lower rates of oocyte maturation and blastocyst formation than cumulus-oocyte complexes with at least one complete layer of cumulus cells (F-COCs; maturation rate: 63, 78 vs 94%; blastocyst rate: 29, 49 vs 80%). Live births were achieved using vitrified blastocysts derived from oocytes taken from vitrified and slow-cooled ovarian tissue heterotypic allografts. Successful production of healthy offspring from these vitrified blastocysts suggests that this technique should be considered as a useful stage to pause in the assisted reproduction pathway. This provides an alternative protocol for restoring fertility and offering cancer patients a better indication of their chances of pregnancy and live birth.

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Biosafety in Embryos and Semen Cryopreservation, Storage, Management and Transport

Bielański¹

2014

Reproductive Sciences in Animal Conservation

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Follicle survival and growth to antral stages in short-term murine ovarian cortical transplants after Cryologic solid surface vitrification or slow-rate freezing

Cited by 31 publications

References 46 publications

Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice

Biosafety in Embryos and Semen Cryopreservation, Storage, Management and Transport

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