Folic Acid Supplementation Attenuates Chronic Hepatic Inflammation in High‐Fat Diet Fed Mice

Sid, Victoria; Shang, Yue; Siow, Yaw L.; Hewage, Susara Madduma; House, James D.; Karmin, O

doi:10.1002/lipd.12084

Cited by 25 publications

(19 citation statements)

References 38 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have found that oral iron supplementation improved iron status, but also increased oxidative stress, even in women with low iron stores [24,25]. Interestingly, Tiwari et al [26] observed that although treatment with iron and folic acid has remarkable efficacy in terms of hemoglobin, it may also lead to impaired liver function in pregnant anemic women. It has recently been shown that folic acid ameliorates liver inflammatory processes [27] and hepatic steatosis [28].…”

Section: Discussionmentioning

confidence: 99%

Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

Suliburska

Skrypnik

Chmurzyńska

2019

Biol Trace Elem Res

View full text Add to dashboard Cite

Although simultaneous supplementation with iron and folic acid is justified, the potential interactions between these micronutrients are unknown. The aim of this study was to determine the effects of oral iron and folic acid, administered together or separately, on iron concentration in tissues in rats with a deficiency of both these micronutrients. In the first stage of the experiment (28 days), 150 8-week-old female Wistar rats were randomly assigned to a control group (C; n = 30) fed the standard diet and to a study group (n = 120) fed a diet deficit in iron and folate. The study group was then randomly divided to four groups: D group fed a deficit diet, FE group fed a deficit diet with iron gluconate, the FOL group fed a deficit diet with folate acid, and the FEFOL group fed a deficit diet with iron gluconate and folate acid. After 2, 10, and 21 days of supplementation, ten animals from each group were killed. Morphological parameters were measured in whole blood. Iron concentration was assayed in serum, liver, spleen, pancreas, heart, and kidneys. Folic acid supplementation more significantly decreased iron concentrations in the pancreas and spleen than in the D group after 10 and 21 days of supplementation. Moreover, the combination of iron with folic acid markedly decreased iron levels in the liver and spleen, in comparison with iron alone, after 10 and 21 days of the experiment. In conclusion, folic acid affects iron status in female rats deficient in these micronutrients in moderate and long-term supplementation.

show abstract

Section: Discussionmentioning

confidence: 99%

Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

Suliburska

Skrypnik

Chmurzyńska

2019

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…Chronic consumption of high-fat diets (HFD) is a major contributor to the development of obesity and metabolic abnormalities. In our previous studies, we observed an increased body weight gain and metabolic abnormalities (hyperlipidemia, hyperglycemia) in mice fed a HFD for 5-12 weeks (11)(12)(13)(14)(15)(16). Recent studies demonstrated renal injury in dietinduced obese mice, a murine model of CKD (7,17).…”

Section: Introductionmentioning

confidence: 93%

Inhibition of Inflammatory Cytokine Expression Prevents High-Fat Diet-Induced Kidney Injury: Role of Lingonberry Supplementation

et al. 2020

Self Cite

View full text Add to dashboard Cite

Chronic low-grade inflammation is a major stimulus for progression of chronic kidney disease (CKD) in individuals consuming high-fat diet. Currently, there are limited treatment options for CKD other than controlling the progression rate and its associated complications. Lingonberry (Vaccinium vitis-idaea L.) is rich in anthocyanins with demonstrated anti-inflammatory effect. In the current study, we investigated the potential renal protective effect of lingonberry and its anthocyanin (cyanidin-3-glucoside) in high-fat diet fed obese mice and in human proximal tubular cells. Prolonged consumption of high-fat diets is strongly associated with obesity, abnormal lipid and glucose metabolism. Mice (C57BL/6J) fed a high-fat diet (62% kcal fat) for 12 weeks developed renal injury as indicated by an elevation of blood urea nitrogen (BUN) level as well as an increase in renal kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL) and renin expression. Those mice displayed an activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and increased expression of inflammatory cytokines-monocyte chemoattractant-1 (MCP-1), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) in the kidneys. Mice fed a high-fat diet also had a significant elevation of inflammatory cytokine levels in the plasma. Dietary supplementation of lingonberry for 12 weeks not only attenuated high-fat diet-induced renal inflammatory response but also reduced kidney injury. Such a treatment improved plasma lipid and glucose profiles, reduced plasma inflammatory cytokine levels but did not affect body weight gain induced by high-fat diet feeding. Lingonberry extract or its active component cyanidin-3-glucoside effectively inhibited palmitic acid-induced NF-κB activation and inflammatory cytokine expression in proximal tubular cells. These results suggest that lingonberry supplementation can reduce inflammatory response and prevent chronic kidney injury. Such a renal protective effect by lingonberry and its active component may be mediated, in part, through NF-κB signaling pathway.

show abstract

“…Asam folat juga dapat mengurangi tingkat sirkulasi mediator proinflamasi pada individu yang kelebihan berat badan dan pada pasien hiperhomosisteinemia (Solini et al, 2006;Wang et al, 2005). Pada penelitian pemberian asam folat oleh Sid et al (2018) pada tikus yang diberi makan HFD (High Fat Diet) yang dapat mengakibatkan fatty liver, mengungkapkan bahwa suplemen asam folat mengurangi peradangan hati pada tikus tersebut.…”

Section: Pembahasanunclassified

“…Kemampuan asam folat untuk menekan respons radang hati menunjukkan mikronutrien tersebut memiliki potensi terapi untuk manajemen fatty liver. Penelitian Sid et al (2018) menguatkan hasil pada pelitian ini bahwa pemberian asam folat mampu menekan stres oksidatif yang terjadi akibat pestisida karbamat, akan tetapi hasilnya tidak dapat mendekati kelompok normal walaupun telah berbeda signifikan jika dibandingkan dengan kelompok P1 induksi karbamat. Pemberian asam folat pada penelitian Sid et al (2018) diketahui selama 8 minggu atau selama minimal 56 hari, sementara dalam penelitian ini hanya dilakukan pemberian asam folat selama 14 hari.…”

Section: Pembahasanunclassified

“…Penelitian Sid et al (2018) menguatkan hasil pada pelitian ini bahwa pemberian asam folat mampu menekan stres oksidatif yang terjadi akibat pestisida karbamat, akan tetapi hasilnya tidak dapat mendekati kelompok normal walaupun telah berbeda signifikan jika dibandingkan dengan kelompok P1 induksi karbamat. Pemberian asam folat pada penelitian Sid et al (2018) diketahui selama 8 minggu atau selama minimal 56 hari, sementara dalam penelitian ini hanya dilakukan pemberian asam folat selama 14 hari. Hal tersebut menunjukkan bahwa perbedaan lama pemberian berpengaruh pada efek antioksidan sebagai pencegahan terjadinya kerusakan pada hati, dalam penelitian ini pemberian asam folat selama 14 hari terbukti dapat mencegah kerusakan pada hati yang diinduksi karabamat akan tetapi pemulihannya tidak dapat mendekati kelompok normal.…”

Section: Pembahasanunclassified

See 1 more Smart Citation

Hepatoprotector Effect of Coconut Water (Cocos nucifera L.) and Folic Acid to the Liver Histopathological Desccription of Pregnant Wistar Female Rats (Rattus norvegicus) Induced by Carbamate

Ridho

Prasetyo²,

Hairrudin³

2020

AMS

View full text Add to dashboard Cite

Abstract Carbamate is an insecticide with a working mechanism that inhibits the enzyme acetylcholineestrase (AChE). Obstacles to AChE will cause the formation of excessive free radicals in the body causing oxidative stress and causing lipid peroxidation in body cells, including hepatocyte cells in the liver. Pregnant women have a change in detoxification activity in the liver due to exposure to xenobiotic substances during pregnancy causing a decrease in cytochrome P450 1A2 (CYP1A2) which will cause an increase in free radicals and can damage liver cells. Coconut water contains antioxidants that can neutralize free radicals in the body. While folic acid can encourage improvement in morphology of liver cells. The purpose of this study was to determine the hepatoprotector effect of coconut water and folic acid on the histopathology of the liver of pregnant female wistar rats induced by carbamate. The design of this study is true experimental with a post test only control group design. The sampling method used is simple random sampling. The number of samples in this study were 28 rats divided into 4 groups K (aquades), P1 (carbamate), P2 (carbamate and coconut water), and P3 (carbamate and folic acid). At the end of the study rat liver was taken to then become histological preparations. The One Way ANOVA test results showed a significant difference between groups compared (p <0.05). Post hoc LSD test results showed that group P1 had damaged liver histopathology, there were significant differences with group K and P3 (p <0.05), but there were no significant differences when compared with group P2 (p = 0.826). Meanwhile, the P3 group had significant differences when compared with all groups (p <0.05). The conclusion of this study is that giving coconut water cannot prevent liver damage due to carbamate induction, while folic acid has been shown to prevent liver damage due to carbamate induction, from liver histopathology. Keywords: carbamate, coconut water, folic acid, liver histopathology

show abstract

Folic Acid Supplementation Attenuates Chronic Hepatic Inflammation in High‐Fat Diet Fed Mice

Cited by 25 publications

References 38 publications

Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

Folic Acid Affects Iron Status in Female Rats with Deficiency of These Micronutrients

Inhibition of Inflammatory Cytokine Expression Prevents High-Fat Diet-Induced Kidney Injury: Role of Lingonberry Supplementation

Hepatoprotector Effect of Coconut Water (Cocos nucifera L.) and Folic Acid to the Liver Histopathological Desccription of Pregnant Wistar Female Rats (Rattus norvegicus) Induced by Carbamate

Contact Info

Product

Resources

About