Folic Acid Deficiency and Hepatic DNA Synthesis.

Abstract

FOLATE AND DNA SYNTHESIShold true when stimuli other than endotoxin are used to produce EP. Third, the semiquantitative data obtained by testing EPor any other substance employed in pyrogen assays-improves the accuracy of these assays and yields more meaningful information for comparison of different pyrogenic substances. Fourth, providing it is clearly defined, the concept of the minimum pyrogenic dose is useful in standardizing the pyrogenic response, and to compare data from different laboratories.It should be realized that, as a form of bioassay, fever is a relatively gross measurement and depends on many variables inherent in the recipient, including the species of animal employed(5). It seems unlikely that differences between individual recipients or even within the same recipient will ever be eliminated. For this reason alone, attempts to control the procedures employed in this assay are indicated. Summary.A semiquantitative assay for endogenous pyrogen in serum is described. The test depends on using at least 3-4 recipients at at least 4 ranges of dosage, and the use of the fever index rather than height of the fever curve. This permits construction of a dose response curve which delineates the sensitive range of pyrexia. The minimum pyrogenic dose can then be calculated and should serve as a useful standard for comparison of different pyrogenic substances.

Folate Deficiency and Pancreatic Acinar Cell Function

“…These results are in agreement with studies by Leevy et a/. (24) which indicated that deficiencies of folate, vitamin BI2, and vitamin B6 diminished DNA synthesis whereas deficiencies of thiamin, riboflavin, nicotinic acid, pantothenic acid, and biotin did not impair DNA synthesis in the liver (5). There was no significant change in total RNA, protein content, and amylase content or amylase secretion.…”

Effect of Dietary Conjugated Linoleic Acid on Liver Regeneration after a Partial Hepatectomy in Rats

“…After these sequential events have been com pleted, it is believed that DNA synthesis in hepatocytes reaches a peak around 1d after PH, and then substan tial cell proliferation begins. Within 3-14d, organ weight has been shown to be restored to the original mass (12)(13)(14). In this experiment, in spite of the slight liver enlargement at the time of PH, liver weight for the CLA fed group was significantly lower than control rats at 7-d post PH.…”