Folic acid abnormalities in iron deficiency: the mechanism of decreased serum folate levels in rats

Toskes, Phillip P.; Smith, George W.; Bensinger, Thomas A.; Giannella, Ralph A.; Conrad, Marcel E.

doi:10.1093/ajcn/27.4.355

Cited by 23 publications

(11 citation statements)

References 23 publications

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“…The continuous increase in MCV after 8 and 14 days of supplementation may be explained by folic acid deficiency triggered by a burst of erythropoiesis in the bone marrow. Indeed, such high doses of iron might have created an "extra need" for folic acid for red blood cell synthesis, which was not covered by our diet [8][9][10]. This study therefore raises the question of the advantage of giving high doses of iron to the control group in studies on iron deficiency in the rat.…”

Section: Discussionmentioning

confidence: 93%

Effects of doses and duration of iron supplement on iron deficiency in rats.

Dhur¹,

Galán²,

Herçberg³

1989

J. Clin. Biochem. Nutr.

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SummaryThe effects of 3 doses and 2 durations of iron supplement on iron-deficient rats were investigated. Two groups of 15 rats were given, respectively, a low iron-containing diet (4-5 mg Fe/kg diet) and a medium iron-containing diet (15 mg Fe/kg diet) for 4 weeks. At that time the first group was anemic and the second group was moderately irondeficient, as shown by their iron status parameters. The two groups were then each divided into 3 subgroups of 5 rats each that received the low iron-containing diet supplemented with 35, 150, or 250 mg of iron in the ferrous sulfate form per kg diet. Changes in their iron status and mean body weight was followed after 8 and 14 days of supplementation. Results showed that, after 14 days of supplementation, a dose of 35 ppm was not sufficient to normalize hematological parameters. However, doses of 150 and 250 mg Fe/kg diet normalized all hematological parameters after 8 days of diet, and corrected the decrease in body weight gain. Key Words. iron deficiency, iron supplementation, rats, sulfate iron Studies on iron deficiency in the rat often include a basal iron-deficient diet containing less than 5 mg Fe/kg diet and a. "control" consisting of the basal iron-deficient diet supplemented with iron in the form of sulfate iron (FeSO4 7H2O) at a high concentration, i.e., up to 250 mg Fe/kg diet. However, the AINC (American Institute of Nutrition Ad Hoc Committee) has recommended a 35 mg Fe/kg diet for growing rats [1].In previous experiments, we studied weanling rats fed different iron-containing diets (basal iron-deficient diet supplemented with sulfate iron) during a 4-to 6-week period and we controlled their iron status. Our results showed that the group consuming 35-40 mg Fe/kg had a normal iron status and that ferrous sulfate

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Section: Discussionmentioning

confidence: 93%

Effects of doses and duration of iron supplement on iron deficiency in rats.

Dhur¹,

Galán²,

Herçberg³

1989

J. Clin. Biochem. Nutr.

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“…La concentracion intraeritrocitaria se reduce despues de una terapia con hierro 14 -19 ' 20 . En ratas con deficiencia de hierro se ha demostrado que no existe alteracion de la absorcion del acido folico, cambios en la flora intestinal o secuestro de folato por estos microorganismos de Pedlatrfa intestinales 18 . For estas razones, inicialmente las alteraciones de los niveles de folato sericos se atribuyeron a un posible atrapamiento hepatico del acido folico, con el consiguiente impedimento del paso de este a la circulacion, lo que se revertiria con la terapia con hierro 17 -Estas anomah'as tambien se ban pretendido explicar como consecuencia de un aumento de los requerimientos de acido folico en la deficiencia de hierro 18 .…”

Section: Resultados Discusionunclassified

Folato Sérico y Eritrocitario en el Lactante: Influencia de la Nutrición de Hierro

Anderson²,

A³

et al. 1986

Rev. chil. pediatr.

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Term infants, were examined at 9 (n=215) and 15 (n=198) months of age for levels of serum and red cell folate, hemoglobin, serum iron, total iron binding capacity, free erythrocyte protoporphyrin and serum ferritm. Mean erythrocyte folate levels were consistently higher (p < 0,005) in iron deficient anemic infants. No diferences were noted in serum folate levels. These results suggest, assuming similar intakes of folate, that folate stores depend on the sufficiency of iron nutrition. Iron deficient erythropoieses determines less utilization of folate stores.

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“…Vitale et al (1966) reported the effects of an iron-deficient diet on rats given folic acid supplements and concluded that folate deficiency could be induced by dietary iron deficiency. Similarly Toskes et al (1974) and Saraya et al (1971) reported reduced serum folate levels in iron deficiency. In addition, a metabolic block in folate metabolism in irondeficient subjects has been postulated, possibly due to the iron dependence of the enzyme formimino-transferase (Vitale et al 1966).…”

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confidence: 90%