Foliar ammonium accumulation as an index of stress in plants

Barker, Allen V.

doi:10.1080/00103629909370193

Cited by 26 publications

(11 citation statements)

References 22 publications

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“…accumulation was reported to increase linearly with respect to external NH 4 ? supply, thus being considered as an index of stress in plants (Barker 1999 ? on free NH 4 ?…”

Section: Resultsmentioning

confidence: 99%

The differing responses of two Arabidopsis ecotypes to ammonium are modulated by the photoperiod regime

Shi

2010

Acta Physiol Plant

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Responses to excessive ammonium (NH 4 ? ) were compared between two Arabidopsis ecotypes (Col-0, JA22) with respect to different photoperiods in hydroponics. In this study, we showed that external extra NH 4 ? led to severe growth suppression, accumulations of free NH 4 ? and amino acids and increased the activities of glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in shoots of the two Arabidopsis ecotypes. However, the levels of free NH 4 ? and total amino acids increased, whereas the activities of GS, NADH-dependent glutamate synthase and GDH decreased under the continuous light when compared with the light (16 h)-dark (8 h) cycle photoperiod. Statistical analyses suggested that strong correlations exist among the growth reduction, accumulations of free NH 4 ? , total amino acids and levels of GS activity in shoots under the high NH 4 ? stress regardless of the photoperiod regimes. Interestingly, under the continuous light, Col-0 showed more resistant to such growth reduction and maintained about onefold higher capability of converting excess free NH 4 ? into amino acids, with onefold higher GS activity induced by the external NH 4 ? when compared with JA22. In contrast, these differences were abolished between Col-0 and JA22 under the lightdark cycle condition. Taken together, our results conclude that the sensitivity to NH 4 ? of Col-0 and JA22 is changed between the continuous light and the light-dark cycle photoperiod, which is correlative to the alteration of the GS activity in shoots.

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“…accumulation was reported to increase linearly with respect to external NH 4 ? supply, thus being considered as an index of stress in plants (Barker 1999 ? on free NH 4 ?…”

Section: Resultsmentioning

confidence: 99%

The differing responses of two Arabidopsis ecotypes to ammonium are modulated by the photoperiod regime

Shi

2010

Acta Physiol Plant

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“…Excessive NH 4 + accumulation is the major cause for injury of plants in many unfavorable conditions (Barker, 1999; Hoai et al, 2003; Nguyen et al, 2005). In this study, NH 4 + significantly accumulated in Col-0 under (NH 4 ) 2 SO 4 conditions (Fig.…”

Section: Discussionmentioning

confidence: 99%

NRT1.1-dependent NH4+ toxicity in Arabidopsis is associated with disturbed balance between NH4+ uptake and assimilation

Jian

Liao

Song

et al. 2017

Preprint

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“…, 2007; Ta and Joy, 1986; Wong et al. , 2004), and accumulation is generally viewed as an index of stress in plants (Barker, 1999a,b; Britto and Kronzucker, 2002). Although the physiological significance of up‐regulating the biosynthesis of ASN is not fully understood, it is likely that an inability to detoxify would, directly or indirectly, affect stress tolerance in the field.…”

Section: Discussionmentioning

confidence: 99%

Tuber‐specific silencing of asparagine synthetase‐1 reduces the acrylamide‐forming potential of potatoes grown in the field without affecting tuber shape and yield

Chawla

Shakya

Rommens³

2012

Plant Biotechnology Journal

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SummarySimultaneous silencing of asparagine synthetase (Ast)-1 and -2 limits asparagine (ASN) formation and, consequently, reduces the acrylamide-forming potential of tubers. The phenotype of silenced lines appears normal in the greenhouse, but field-grown tubers are small and cracked. Assessing the effects of silencing StAst1 and StAst2 individually, we found that yield drag was mainly linked to down-regulation of StAst2. Interestingly, tubers from untransformed scions grafted onto intragenic StAst1 ⁄ 2-silenced rootstock contained almost the same low ASN levels as those in the original silenced lines, indicating that ASN is mainly formed in tubers rather than being transported from leaves. This conclusion was further supported by the finding that overexpression of StAst2 caused ASN to accumulate in leaves but not tubers. Thus, ASN does not appear to be the main form of organic nitrogen transported from leaves to tubers. Because reduced ASN levels coincided with increased levels of glutamine, it appears likely that this alternative amide amino acid is mobilized to tubers, where it is converted into ASN by StAst1. Indeed, tuber-specific silencing of StAst1, but not of StAst2, was sufficient to substantially lower ASN formation in tubers. Extensive field studies demonstrated that the reduced acrylamide-forming potential achieved by tuber-specific StAst1 silencing did not affect the yield or quality of field-harvested tubers.

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Foliar ammonium accumulation as an index of stress in plants

Cited by 26 publications

References 22 publications

The differing responses of two Arabidopsis ecotypes to ammonium are modulated by the photoperiod regime

The differing responses of two Arabidopsis ecotypes to ammonium are modulated by the photoperiod regime

NRT1.1-dependent NH4+ toxicity in Arabidopsis is associated with disturbed balance between NH4+ uptake and assimilation

Tuber‐specific silencing of asparagine synthetase‐1 reduces the acrylamide‐forming potential of potatoes grown in the field without affecting tuber shape and yield

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