Folate-Conjugated Chitosan-Pluronic P123 Nanogels: Synthesis and Characterizations towards Dual Drug Delivery

Abstract

In this study, a self-assembled nanogel-based pluronic P123-grafted chitosan-folate (CP-FA) was fabricated as a paclitaxel/curcumin codelivery system. 1 H-NMR and TGA proved that the fabricating method of CP-FA was successful. Dynamic light scattering (DLS), zeta potentials, and transmission electron microscopy (TEM) exposed that CP-FA nanoparticles had a uniform size with a diameter of around 16:27 ± 2:01 nm in the colloidal solution and had better sustainable stability at a lower concentration than P123 due to the moderate positive potential value (39:43 ± 3:45 mV) and the lower critical micelle concentration (0.036 mg/ml). Dual drugs were loaded with CP-FA nanogels via self-assembly by the hydrophobic interaction between both hydrophobic therapeutic compounds (PTX and Cur) and the hydrophobic segment of the P123 copolymer. The high hydrophobicity of the segment induced a great loading efficacy of up to 98:63 ± 0:42 of PTX and 97:82 ± 0:48 of Cur. In addition, the CP-FA nanogels exposed superior effects in a controlled release of these encapsulated therapeutic compounds for a long period of time. The anticancer activity of the dual-drug delivery system was evaluated using human breast cancer cell lines (MCF-7) via the IC50 value to compare with the PTX-loading CP-FA nanogel. The obtained results suggested that CP-FA/PTX-Cur displayed a remarkable improvement in anticancer activity at an IC50 value of 5:74 ± 0:23 nM which was higher than that of CP-FA/PTX (IC50 = 8:20 ± 1:41 nM) due to the synergistic effect of both PTX and Cur. Thereby, a dual-drug deliverysystem-based CP-FA of PTX and Cur has been proposed as a promising candidate in cancer therapy.

Dual Arginine and Glutamic Amino Acids Delivery Effectiveness of Injectable Chitosan-Poloxamer P407 towards Wound Healing Application

“…The cellulose dialysis membranes (molecular weight cut-off of 12-14 and 3.5 kDa) obtaining from Repligen were used to purify products. Mononitrophenyl formate-activated Poloxamer (NPC-P407-OH) was prepared in our previous studies at the Institute for Applied Materials Science as described [12,13]. All other chemicals were purchased from Thermo Fisher Scientific (Waltham, MA) or Fisher (USA) or of the analytical grade.…”

“…Poloxamer, an amphiphilic, thermo-sensitive, and FDA-approved Triblock copolymer of poly (ethylene oxide) and poly (propylene oxide), is one of the most studied platforms for the preparation of highly efficient hydrogels [5,6,8]. Chitosan-grafted poloxamer is widely exploited in cartilage regeneration, wound healing, burn healing, and anti-cancer drug delivery [7,[9][10][11][12][13].L-Arginine (Arg) is an essential amino acid that helps to prevent and treat circulatory diseases, alleviate fatigue, and stimulate the immune system. Arg is also known as endothelial nitric oxide synthase enzyme (eNOS) substrate [14], responsible for Nitric Oxide (NO) synthesis [15].…”

“…Poloxamer, an amphiphilic, thermo-sensitive, and FDA-approved Triblock copolymer of poly (ethylene oxide) and poly (propylene oxide), is one of the most studied platforms for the preparation of highly efficient hydrogels [5,6,8]. Chitosan-grafted poloxamer is widely exploited in cartilage regeneration, wound healing, burn healing, and anti-cancer drug delivery [7,[9][10][11][12][13].…”

Vitamin E TPGS-Poloxamer Nanoparticles Entrapping a Novel PI3Kα Inhibitor Potentiate Its Activity against Breast Cancer Cell Lines

“…Poloxamers are amphiphilic triblock copolymers composed of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) (PEO-PPO-PEO) [17,18]. They are biocompatible polymers that are used in various applications, especially as nanocarriers for hydrophobic drugs [19]. Poloxamers have also been combined with other polymers to improve drug loading, impart NP stability, and enhance drug solubility [18].…”

Therapeutic Nanomaterials for Neurological Diseases and Cancer Therapy

“…Nanomedicine is a promising field that uses nanosized (10-100 nm) materials to facilitate the diagnosis and treatment of diseases. These nanomaterials with being used as a drug are also used as a carrier, a scaffold, or an imaging agent [1][2][3]. In common, all these applications could benefit from the extraordinary properties of nanomaterials, such as high specific surface area, transformable shape and size, and tunable chemical reactivity.…”