Focusing of alkali earth metals in ligand step gradient

Šišperová, Eliška; Glovinová, Eliška; Budilová, Jana; Pospı́chal, Jan

doi:10.1016/j.chroma.2011.03.023

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…The separation is based on differences in metal‐EDTA complex formation constants and the formation of metal ion substitution reaction in a continuous moving chelation boundary (MCB). Šišperová et al combined IEF, moving boundary electrophoresis and ITP for enhancement of alkali earth metals (Mg 2+ , Ca 2+ , Sr 2+ and Ba 2+ ) . This combination offered a five‐ to 29‐fold improvement in detection sensitivity.…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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CE has been alive for over two decades now, yet its sensitivity is still regarded as being inferior to that of more traditional methods of separation such as HPLC. As such, it is unsurprising that overcoming this issue still generates much scientific interest. This review continues to update this series of reviews, first published in Electrophoresis in 2007, with updates published in 2009 and 2011 and covers material published through to June 2012. It includes developments in the field of stacking, covering all methods from field amplified sample stacking and large volume sample stacking, through to isotachophoresis, dynamic pH junction and sweeping. Attention is also given to online or inline extraction methods that have been used for electrophoresis.

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Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

et al. 2013

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“…The technique was illustrated with numerical simulations and its effectiveness was demonstrated on the example of separation of a 1 kbp DNA ladder on a microchip combined with an epifluorescent microscope equipped with a LED lamp and a CCD camera (LE: 150 mM HCl, 300 mM imidazole, 100 μM rhodamine‐6G; anionic TE: 20 mM N ‐(tri(hydroxymethyl) methyl)glycine, imidazole; cationic TE: 30 mM arginine‐HCl; sample added to LE with a final concentration of 50 μg/L; 0.75% w/w hydroxyethylcellulose (HEC) (mixed with HCl), 1% w/w PVP in all zones). Šišperová et al described the use of a commercial column‐switching instrument with conductivity detection for stacking and selective preconcentration of alkali earth metal chelates with subsequent ITP analysis. The cations were continuously electrokinetically dosed and focused in a ligand step gradient created by the addition of a counterionic complexing agent to the LE.…”

Section: Instrumentation and Techniquesmentioning

confidence: 99%

Recent progress in analytical capillary isotachophoresis

2012

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This review brings a survey of the literature on analytical isotachophoresis (ITP) from the years 2010-2012. It confirms the fact that ITP alone is not used for analyses frequently but that its online combinations with other methods are of paramount importance. This review shows that the inherent features of the technique and first of all its concentrating ability are still unique for reaching high sensitivity and efficient sample cleanup in analytical applications. The part devoted to theory is mostly represented by computer simulations and confirms the power and significance of this approach. The section oriented at instrumentation and techniques shows the advantages of ITP in column combinations and microchip techniques. The chapter reviewing the applications is categorized according to the techniques applied, viz., column switching, on line ITP-CZE and on-chip analyses. The final part of the review is devoted to the nearly omnipresent electrophoresis principle of transient isotachophoresis, and to the advantages that it may offer for detection and sampling. In all parts, the significance of the operational conditions is also considered and where possible, the electrolyte system is explicitly presented.

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“…CCD was used in chiral analysis by CE , however, to the best of our knowledge, its use in separations based on the LE mechanism in CE has not been reported yet. Conductivity detection is commonly used in isotachophoresis, for example, recently utilized in a method for the separation of alkali earth metals with chelating agents . CCD provides even better results than indirect UV detection when carefully optimized.…”

Section: Introductionmentioning

confidence: 99%

Enantioseparation of tartaric acid by ligand‐exchange capillary electrophoresis using contactless conductivity detection

Knob

Petr

Ševčı́k

et al. 2013

J of Separation Science

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A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with L-hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl2, 14 mM trans-4-hydroxy-L-proline, and 100 mM ε-aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide-coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from ε-aminocaproic acid to L-histidine. With detection limits of about 20 μM, the method was applied to the analysis of wine and grape samples; only L-tartaric acid was found.

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Focusing of alkali earth metals in ligand step gradient

Cited by 5 publications

References 32 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2010–2012)

Recent progress in analytical capillary isotachophoresis

Enantioseparation of tartaric acid by ligand‐exchange capillary electrophoresis using contactless conductivity detection

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