Focused-Pulsed sludge pre-treatment increases the bacterial diversity and relative abundance of acetoclastic methanogens in a full-scale anaerobic digester

Zhang, Husen; Banaszak, James E.; Parameswaran, Prathap; Alder, Jared M.; Krajmalnik‐Brown, Rosa; Rittmann, Bruce E.

doi:10.1016/j.watres.2009.07.034

Cited by 133 publications

(58 citation statements)

References 26 publications

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“…The genus Ruminococcus commonly present in the rumen was also identified in the clone sequences obtained with primer cel48. This genus has previously also been found in anaerobic digesters [32]. For cel5, the clones obtained all belonged to the phyla Bacteroidetes and Firmicutes, which is in agreement with other studies on biogas reactors.…”

Section: Clone Libraries and Phylogenetic Analysissupporting

confidence: 91%

“…This clone was most closely related to Ruminococcus champanellensis (61.8% identity), a cellulolytic bacterium isolated from human faeces that ferments cellulose, cellobiose and xylan, produces acetate and succinate as the main fermentation products and grows optimally from 33°C to 39°C (optimum 39°C) [41]. In a previous study, the abundance of Ruminococcus in a biogas digester was shown to increase after pre-treatment of sludge from a wastewater plant [32]. A Ruminococcus sp.-like clone was also recovered in another biogas process operating with synthetic wastes under thermophilic conditions [42].…”

Section: Cel48mentioning

confidence: 83%

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Biogas production from wheat straw: community structure of cellulose-degrading bacteria

2013

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Background: Wheat straw is one of the most abundant crop residues produced in the world, making it highly interesting as a substrate for biogas production. However, due to the complex structure, its degradability and gas yield are low. The degradability can be improved by pre-treatment, making the material more accessible to microbial degradation. Methods: To investigate the microbial response to straw as a feed stock for biogas production, this study examined the community structure of cellulose-degrading bacteria in lab-scale biogas digesters operating with manure, alone or in co-digestion with straw, with and without pre-treatment (steam-explosion) at different temperatures. The community was studied by targeting the functional gene encoding glycoside hydrolases of families 5 and 48 using T-RFLP, clone libraries and qPCR. Results: In general, bacteria belonging to the phyla Firmicutes and Bacteroidetes dominated the cellulose-degrading bacteria community in all digesters. The degree of similarity to the characterised bacteria was often low, and some clones were more closely related to the uncultured bacteria. The addition of straw, pre-treatment of straw and increasing operating temperature all affected the cellulose-degrading community structure, with differing responses in the cel48 and cel5 communities. Both communities changed in response to temperature, while only the cel5 community was affected by the addition of straw and cel48 community by straw pre-treatment. Conclusions: The addition of straw, pre-treatment of straw and operating temperature all affected the cellulosedegrading community in biogas digesters, but there were no major differences in the digester performance and gas yield.

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Section: Clone Libraries and Phylogenetic Analysissupporting

confidence: 91%

Section: Cel48mentioning

confidence: 83%

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

2013

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“…Although some archaea, such as the halophiles and thermophiles, are restricted to extreme environments, the methanogens are widespread and are found in nearly every habitat in which anaerobic biodegradation of organic compounds occurs, including the human and animal intestinal tracts ( 4,5 ), freshwater and marine sediments, and anaerobic waste digesters ( 4 ). Acidophilic methanogens are ubiquitous in peatlands, which are among the largest natural sources of atmospheric methane ( 6 There is growing evidence that host / microbial interactions within the gut can have a profound impact on human health and disease; in fact, the intestinal microfl ora have been shown to infl uence the innate physiology, biochemistry, immunology, maturation of the vasculature, and gene expression in a host.…”

Section: Archaeamentioning

confidence: 99%

Methanogens in Human Health and Disease

Pimentel¹,

Gunsalus²,

Rao³

et al. 2012

Am J Gastroenterol Suppl

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“…T. porcinum, a homoacetogenic genus that is enriched with the addition of biomethanation of coke oven gas, represented the remaining 13.4 % of the clones [20]. A study of anaerobic cofermentation of straw and manure suggested that T. porcinum is specialized to produce acetate by utilizing H 2 and CO 2 [21].…”

Section: Discussionmentioning

confidence: 99%

Diversity of archaea and bacteria in a biogas reactor fed with <i>Pennisetum sinese</i> Roxb by 16S rRNA sequence analysis

Zhao¹,

Ai²,

Li³

et al. 2017

Trop. J. Pharm Res

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Focused-Pulsed sludge pre-treatment increases the bacterial diversity and relative abundance of acetoclastic methanogens in a full-scale anaerobic digester

Cited by 133 publications

References 26 publications

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

Biogas production from wheat straw: community structure of cellulose-degrading bacteria

Methanogens in Human Health and Disease

Diversity of archaea and bacteria in a biogas reactor fed with <i>Pennisetum sinese</i> Roxb by 16S rRNA sequence analysis

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