Foccα6, a truncated nAChR subunit, positively correlates with spinosad resistance in the western flower thrips, Frankliniella occidentalis (Pergande)

Wan, Yanran; Yuan, Guangdi; He, Bin; Xu, Baoyun; Xie, Wen; Wang, Shaoli; Zhang, Youjun; Wu, Qingjun; Zhou, Xuguo

doi:10.1016/j.ibmb.2018.05.002

Cited by 42 publications

(44 citation statements)

References 71 publications

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“…In F. occidentalis , a non‐synonymous point mutation, G275E of Foα6 , was first reported to be associated with spinosad resistance and to be functionally validated through introducing the G275E into the Dα6 subunit with the CRISPR/Cas9 system . A truncated Foα6 subunit, positively correlated with spinosad resistance under laboratory conditions, was identified and later confirmed under field conditions . In other insect species, the substitution corresponding to Foα6 G275E mutation was also detected in Spinosad‐resistant T. palmi and T. absoluta .…”

Section: Discussionmentioning

confidence: 94%

“…35 A truncated Fo 6 subunit, positively correlated with spinosad resistance under laboratory conditions, was identified and later confirmed under field conditions. 29 In other insect species, the substitution corresponding to Fo 6 G275E mutation was also detected in Spinosad-resistant T. palmi and T. absoluta. 22,23 Truncated transcripts of nAChR Bd 6 were associated with spinosad resistance in B. dorsalis.…”

Section: Discussionmentioning

confidence: 97%

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Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

Wang

et al. 2019

Pest Management Science

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BACKGROUND The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides some advantages over other reverse genetic techniques to investigate the causal relationship between insecticide resistance phenotype and candidate gene. Several studies published to date point to the nicotinic acetylcholine receptor (nAChR) α6 subunit strongly associated with spinosyns resistance in insects, including Plutella xylostella. However, reverse genetic verification of the P. xylostella nAChRα6 has not yet been achieved via an in vivo approach. RESULTS Here, we successfully constructed a homozygous strain (Pxα6‐KO) with a 2‐nt deletion mutation of nAChRα6 by CRISPR/Cas9 coupled with non‐homologous end joining approach in P. xylostella. The manipulated mutation results in a frame shift in the open reading frame of transcripts, which produces a predicted protein truncated in the TM3–TM4 loop region. When compared to the background strain IPP‐S, the knockout strain Pxα6‐KO exhibited 229‐ and 1462‐fold resistance to spinosad and spinetoram, respectively, but no or limited (resistance ratios <3‐fold) effects on the toxicities of imidacloprid, abamectin, β‐cypermethrin, indoxacarb, metaflumizone and chlorantraniliprole. Furthermore, the mode of inheritance of the acquired spinetoram resistance was autosomal recessive and significantly linked with the 2‐nt deletion mutation of nAChRα6 in the Pxα6‐KO strain. CONCLUSION In vivo functional investigation demonstrates the causality of the Pxα6 truncating mutation with high levels of resistance to spinosyns in P. xylostella. Our results suggest the Pxα6‐KO strain underlies an autosomal, recessive mode of inheritance for spinetoram resistance, and reinforces the association of this gene to the mode of action of spinosyns. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 97%

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

Wang

et al. 2019

Pest Management Science

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“…Subsequent studies on several insect crop pests have described genetic alterations in nAChR α6 sequences that result in truncated non‐functional proteins leading to resistance. For example, several mutations resulting in mis‐splicing and the introduction of PTCs in nAChR α6 transcripts are associated with spinosad resistance in the diamondback moth, Plutella xylostella, the oriental fruit fly Bactrocera dorsalis , the western flower thrips, Frankliniella occidentalis and the lesser grain borer, Rhyzopertha dominica . In a previous study of a spinosad resistant T. absoluta strain, derived from a population collected in Portugal, resistance was conferred by skipping of exon 3 rather than exon 4, however, in this case the reading frame of the altered Taα6 transcripts was unaffected by the exclusion of exon 3 .…”

Section: Discussionmentioning

confidence: 99%

The evolution of multiple‐insecticide resistance in UK populations of tomato leafminer, Tuta absoluta

Grant

Jacobson²,

Ilias³

et al. 2019

Pest Management Science

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BACKGROUND: The tomato leafminer, Tuta absoluta, is an economically important pest of tomatoes in Europe, Africa, Asia and South America. In the UK this species is controlled using an integrated pest management (IPM) programme which incorporates the insecticides spinosad and chlorantraniliprole. In response to UK grower concerns of loss of efficacy of these compounds at certain sites, insecticide bioassays were performed on five populations collected from four commercial glasshouses and potential mechanisms of resistance investigated. RESULTS:We observed high levels of resistance to spinosad in four of the strains, and in two of these tolerance to chlorantraniliprole. Selection of one of these strains with chlorantraniliprole rapidly resulted in a line exhibiting potent resistance to this compound. Sequencing of messenger RNA encoding the nicotinic acetylcholine receptor (nAChR) 6 subunit, target of spinosad, revealed Ta 6 transcripts in the spinosad-resistant strains that lack exon 4 and encode a highly truncated protein, or contain a triplet deletion in the predicted first transmembrane domain resulting in the loss of a highly conserved amino acid. Sequencing of the ryanodine receptor gene, encoding the target of diamide insecticides, of the chlorantraniliprole-selected line revealed an amino acid substitution (G4903V) that has been previously linked to diamide resistance in populations of T. absoluta in the Mediterranean and South America. CONCLUSION: Taken together our results reveal emerging resistance in UK populations of T. absoluta to two of the most important insecticides used as part of IPM, with significant implications for the control of this species in the UK.

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“…Meanwhile, the TSWV transmission efficiency of spinosad-resistant thrips was not different from that of normal thrips. 23 Considering the prevalence of F. occidentalis resistance to spinosad in the field, 4 where the virus is already present. Spin-R, and Ned strains of F. occidentalis as determined by the TSWV status (virus exposed or non-exposed) of the parental thrips (in groups of 45 females and 15/45 males).…”

Section: Discussionmentioning

confidence: 99%

“…Ivf03 is a laboratory susceptible strain and Spin-R is a spinosad-resistant strain as described by Wan et al 4 Ned, another laboratory susceptible strain, was a gift from Dr. Dick Peters, Wageningen University & Research, in 2006. All three strains were maintained separately on kidney bean pods at 26℃, 70% RH and a 16:8 (L:D) h photoperiod.…”

Section: Frankliniella Occidentalis Strains and Tswv Maintenancementioning

confidence: 99%

Tomato spotted wilt virusmanipulates the reproduction of its insect vector, western flower thrips (Frankliniella occidentalis), to facilitate transmission

Hussain

et al. 2019

Preprint

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BACKGROUND: Tomato spotted wilt virus (TSWV), one of the most devastating viruses of ornamental plants and vegetable crops worldwide, is transmitted by the western flower thrips, Frankliniella occidentalis (Pergande), in a persistent-propagative manner. How TSWV manipulates the reproduction of its vector to enhance transmission and whether infection with TSWV changes the mating behaviour of this thrips vector are not fully understood. RESULTS:In this study, we found that TSWV-exposed thrips, in general, had a significantly longer developmental time than did non-exposed individuals. Such an increase was predominantly seen in adults, a stage associated with dispersal and virus transmission. TSWV-exposed F. occidentalis produced substantially more progeny than did non-exposed thrips. Interestingly, most of the increase in progeny came from an increase in males, a sex with a greater dispersal and virus transmission capability. Specifically, the sex ratio of progeny shifted from female biased (2-7:1) to evenly split or male biased. Regarding mating behaviour, compared to virus-free controls, TSWV-exposed F. occidentalis had significantly longer copulation duration, were more active in males, and remated less often in females. CONCLUSION: These combined results suggest that TSWV alters the reproductive behaviour of its insect vector, F. occidentalis, to promote virus transmission. Consequently, a monitoring program capable of earlier detection of the virus and a reduced economic threshold for vector (thrips) control should be in consideration for the long-term, sustainable management of TSWV.

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Foccα6, a truncated nAChR subunit, positively correlates with spinosad resistance in the western flower thrips, Frankliniella occidentalis (Pergande)

Cited by 42 publications

References 71 publications

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

The evolution of multiple‐insecticide resistance in UK populations of tomato leafminer, Tuta absoluta

Tomato spotted wilt virusmanipulates the reproduction of its insect vector, western flower thrips (Frankliniella occidentalis), to facilitate transmission

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