Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces

Moon, Yea Hwang; Yoon, Mikyeong; Moon, Jung-Sun; Kang, Ji-Hyoun; Kim, Sun-Hun; Yang, Hong-Seo; Kim, Minseok

doi:10.4047/jap.2013.5.3.341

Cited by 17 publications

(9 citation statements)

References 39 publications

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“…As expected in the present study the cellular viability, the number of cells and the surface occupied by cells were shown to increase with time, showing a very good cellular attachment and proliferation on titanium. The results confirmed the good biocompatibility of titanium found in numerous previous studies (Cochran et al 1994;Eisenbarth et al 1996;Gomez-Florit et al 2013;Grossner-Schreiber et al 2006;Hormia and Kononen 1994;Kononen et al 1992;Moon et al 2013;Nothdurft et al 2014;Pae et al 2009). Actually, titanium is a gold-standard material in the field of dental implant prosthodontics mainly due to its high corrosion resistance, low toxicity, very low allergenic potential and good biocompatibility properties (Jorge et al 2013).…”

Section: Discussionsupporting

confidence: 89%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glassceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.

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Section: Discussionsupporting

confidence: 89%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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“…44 Vinculin is a focal adhesion linker protein, and increased vinculin expression is associated with enhanced adhesion strength. 45 Therefore, both the Nano and Micro surfaces could result in a stronger adhesion of HGFs to the implant than Smooth surface, which is beneficial for the stability of connective tissue integration. Taken together, the Nano surface promoted the attachment of both HGECs and HGFs when compared with other surfaces.…”

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confidence: 99%

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Miao¹,

Wang²,

Xu³

et al. 2017

IJN

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Nanotopography modification is a major focus of interest in current titanium surface design; however, the influence of the nanostructured surface on human cell/bacterium behavior has rarely been systematically evaluated. In this study, a homogeneous nanofiber structure was prepared on a titanium surface (Nano) by alkali-hydrothermal treatment, and the effects of this Nano surface on the behaviors of human MG-63 osteoblasts, human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs) were evaluated in comparison with a smooth titanium surface (Smooth) by polishing and a micro-rough titanium surface (Micro) by sandblasting and acid etching. In addition, the impacts of these different surface morphologies on human THP-1 macrophage polarization and Streptococcus mutans attachment were also assessed. Our findings showed that the nanostructured surface enhanced the osteogenic activity of MG-63 cells (Nano=Micro>Smooth) at the same time that it improved the attachment of HGECs (Nano>Smooth>Micro) and HGFs (Nano=Micro>Smooth). Furthermore, the surface with nanotexture did not affect macrophage polarization (Nano=Micro=Smooth), but did reduce initial bacterial adhesion (Nano

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“…FN, which occurs in two forms, namely, the soluble plasma FN in blood and insoluble cellular FN in ECM or on cell surfaces, is a key factor in the compatibility of dental implant materials for connective tissues [40]. VCL is a FA linker protein, and increased VCL expression is associated with enhanced adhesion strength [41]. The high expressions of cellular FN and VCL might also facilitate the adhesion of HGFs on NT10 and NT30.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes

Zeng³

et al. 2020

BioMed Research International

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Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants.

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Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces

Cited by 17 publications

References 39 publications

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes

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