Fly culture collapse disorder: detection, prophylaxis and eradication of the microsporidian parasite<i>Tubulinosema ratisbonensis</i>infecting<i>Drosophila melanogaster</i>

Niehus, Sebastian; Giammarinaro, Philippe; Liégeois, Samuel; Quintin, Jessica; Ferrandon, Dominique

doi:10.4161/fly.20896

Cited by 15 publications

(12 citation statements)

References 26 publications

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“…1e,f). As reported earlier, the progression of infection in this injection paradigm resembles that observed in naturally infested flies, with variable titers measured in the latter 24 . The infection progressively decreased the fitness of the fly in terms of motility, feeding, egg maturation in the ovaries, and egg-laying (Fig.…”

Section: Resultssupporting

confidence: 70%

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Phosphatidic acid as a limiting host metabolite for the proliferation of the microsporidium Tubulinosema ratisbonensis in Drosophila flies

et al. 2019

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Section: Resultssupporting

confidence: 70%

“…2c-f). The impaired excretory function of Malpighian tubules likely accounts for the increased water retention measured in infected flies and hence for their enlarged size 24 .…”

Section: Resultsmentioning

confidence: 99%

Phosphatidic acid as a limiting host metabolite for the proliferation of the microsporidium Tubulinosema ratisbonensis in Drosophila flies

et al. 2019

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“…We also identified the microsporidian Tubulinosema ratisbonensis (e.g. Niehus et al . 2012) in one sample (0.54 RPKM).…”

Section: Resultsmentioning

confidence: 99%

“…On average, 93% of reads (range 70 -98%) could be mapped to Drosophila or likely components of the Drosophila microbial community: Wolbachia made up an average of 0.5% of mapped non-fly reads (range 0.0 -2.9%), other mapped bacterial reads combined were 0.6% (0.0 -3.2%), and microbial eukaryotes were 0.3% (0.0 -3.7%). The eukaryotic microbiota included the fungal pathogen Entomophthora muscae (Elya et al 2018), with reads present in 42 of 167 samples (up to 1.38 reads per kilobase per million reads, RPKM), a novel trypanosomatid distantly related to Herpetomonas muscarum (Sloan et al 2019) with reads present in 80 samples (up to 0.87 RPKM), and the microsporidian Tubulinosema ratisbonensis (Niehus et al 2012), which we detected in one sample (0.54 RPKM). We excluded two virus-like DNA Polymerase B fragments from the analyses below because they consistently co-occurred with a fungus very closely related to Candida (Clavispora) lusitaniae (correlation coefficient on >0.94, p<10 -10 ; Supplementary File S4).…”

Section: Host Species Compositionmentioning

confidence: 87%

The discovery, distribution and diversity of DNA viruses associated withDrosophila melanogasterin Europe

Wallace

Coffman

Gilbert

et al. 2020

Preprint

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Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described in association with the Drosophilidae. This has limited the opportunity to use natural host-pathogen combinations in experimental studies, and may have biased our understanding of the Drosophila virome. Here we describe fourteen DNA viruses detectable by metagenomic analysis of 6.5 thousand pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new Nudiviruses, a new and divergent Entomopox virus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of Galbut virus, an RNA Partitivirus, segregating at very low frequency. Remarkably, we show that Vesanto virus, a small DNA virus previously described as a Bidnavirus, may be composed of up to 12 segments and represents a new lineage of segmented DNA viruses. Only two of the DNA viruses, Kallithea virus (Nudiviridae) and Vesanto virus (Bidna-virus like) are common, being found in 2% or more of wild flies. The other viruses are rare, with many likely to be represented by a single infected fly in the collection. We find that virus prevalence in Europe reflects that seen in publicly-available datasets, with Kallithea virus and Vesanto virus being commonly detectable in data from wild-caught flies and large population cages, and the others being rare or absent. These analyses suggest that DNA viruses are generally rarer than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redress the earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.

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“…Tubulinosema ratisbonensis belongs to the Microsporidia , a group of obligate intracellular parasites that group near the base of the fungal radiation (1). T. ratisbonensis is a pathogen of Drosophila melanogaster, responsible for fly culture collapse (2). This species presents a unique opportunity to study and better understand specific host-parasite interactions and also to decipher the adaptation capacities of this parasite.…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of Tubulinosema ratisbonensis, a Microsporidian Species Infecting the Model Organism Drosophila melanogaster

Polonais

Niehus

Wawrzyniak

et al. 2019

Microbiol Resour Announc

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Fly culture collapse disorder: detection, prophylaxis and eradication of the microsporidian parasiteTubulinosema ratisbonensisinfectingDrosophila melanogaster

Cited by 15 publications

References 26 publications

Phosphatidic acid as a limiting host metabolite for the proliferation of the microsporidium Tubulinosema ratisbonensis in Drosophila flies

Phosphatidic acid as a limiting host metabolite for the proliferation of the microsporidium Tubulinosema ratisbonensis in Drosophila flies

The discovery, distribution and diversity of DNA viruses associated withDrosophila melanogasterin Europe

Draft Genome Sequence of Tubulinosema ratisbonensis, a Microsporidian Species Infecting the Model Organism Drosophila melanogaster

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