Draft Genome Sequence of Tubulinosema ratisbonensis, a Microsporidian Species Infecting the Model Organism Drosophila melanogaster

Polonais, Valérie; Niehus, Sebastian; Wawrzyniak, Ivan; Franchet, Adrien; Gaspin, Christine; Belkorchia, Abdel; Reichstadt, Matthieu; Belser, Caroline; Labadie, Karine; Couloux, Arnaud; Delbac, Frédéric; Peyretaillade, Éric; Ferrandon, Dominique

doi:10.1128/mra.00077-19

Cited by 6 publications

(6 citation statements)

References 22 publications

(23 reference statements)

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“…S3 ) but they share common characteristics including a high molecular weight, an acidic pI and a glutamate and alanine richness. Two PTP3 encoding genes were also identified in the recently sequenced genome of T. ratisbonensis , a species closely related to A. algerae 31 or in the mosquito parasite Edhazardia aedis , whereas a single ptp3 gene was identified in all the other microsporidian genomes sequenced so far 15 .…”

Section: Discussionmentioning

confidence: 99%

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

Fayet

Prybylski

Collin

et al. 2023

Sci Rep

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Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, which is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsporidian Anncaliia algerae. While PTP1 and PTP2 are found on the whole polar tube, PTP3 is present in a large part of the extruded polar tube except at its end-terminal part. On the contrary, PTP4 is specifically detected at the end-terminal part of the polar tube. To complete PTPs repertoire, sequential sporal protein extractions were done with high concentration of reducing agents. In addition, a method to purify polar tubes was developed. Mass spectrometry analysis conducted on both samples led to the identification of a PTP3-like protein (PTP3b), and a new PTP (PTP7) only found at the extremity of the polar tube. The specific localization of PTPs asks the question of their roles in cell invasion processes used by A. algerae.

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Section: Discussionmentioning

confidence: 99%

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

Fayet

Prybylski

Collin

et al. 2023

Sci Rep

Self Cite

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show abstract

“…S2) but they share common characteristics including a high molecular weight, an acidic pI and a glutamate and alanine richness. Two PTP3 encoding genes were also identi ed in the recently sequenced genome of T. ratisbonensis, a species closely related to A. algerae 31 or in the mosquito parasite Edhazardia aedis, whereas a single ptp3 gene was identi ed in all the other microsporidian genomes sequenced so far 15 .…”

Section: Discussionmentioning

confidence: 99%

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

Fayet

Prybylski

Collin

et al. 2023

Preprint

Self Cite

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Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates to vertebrates. The success of their invasion process is based on an original organelle, the polar tube, that is suddenly extruded from the spore to inoculate the sporoplasm into the host cytoplasm. The polar tube is mainly composed of proteins named polar tube proteins (PTPs). A comparative analysis allowed us to identify genes coding for 5 PTPs (PTP1 to PTP5) in the genome of the microsporidian Anncaliia algerae. While PTP1 and PTP2 are found on the whole polar tube, PTP3 is present in a large part of the extruded polar tube except at its end-terminal part. On the contrary, PTP4 is specifically detected at the end-terminal part of the polar tube. To complete PTPs repertoire, sequential sporal protein extractions were done with high concentration of reducing agents. In addition, a method to purify polar tubes was developed. Mass spectrometry analysis conducted on both samples led to the identification of a PTP3-like protein (PTP3b), and a new PTP (PTP6) only found at the extremity of the polar tube. The specific localization of PTPs ask the question of their roles in cell invasion processes used by A. algerae.

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“…This is not a problem if we consider the pangenome but it is more problematic for the core genome [54] especially for organisms such as microsporidia that can be found in multiple ecological niches and therefore present variable gene contents [23,55]. So, to ensure the annotation of new genes, the implementation of an ab initio method is needed and the Glimmer tool which was used for the annotation of several microsporidian genomes was selected [27,28,56,57]. For the best possible prediction, the dataset used for the construction of the Glimmer model must be perfectly reliable.…”

Section: Discussionmentioning

confidence: 99%

MicroAnnot: A Dedicated Workflow for Accurate Microsporidian Genome Annotation

Tournayre,

Polonais,

Wawrzyniak

et al. 2023

Preprint

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With nearly 1,700 species, Microsporidia represent a group of obligate intracellular eukaryotes with veterinary, economic and medical impacts. To help in understanding the biological functions of these microorganisms, complete genome sequencing is used routinely. Nevertheless, the proper prediction of their gene catalogue is challenging due to taxon-specific evolutionary features. As innovative genome annotation strategies are needed to obtain a representative snapshot of the overall lifestyle of these parasites, the MicroAnnot tool, a dedicated workflow for microsporidian sequence annotation using data from curated databases of accurately annotated microsporidian genes, has been developed. Furthermore, specific modules have been implemented to perform small genes (&lt; 300bp) and transposable element identification. Finally, functional annotation was performed using the signature-based InterProScan software. MicroAnnot’s accuracy has been verified by the re-annotation of four microsporidian genomes for which structural annotation had previously been validated. With its comparative approach and transcriptional signal identification method, MicroAnnot provides accurate prediction of translation initiation sites, efficient identification of transposable elements, as well as high specificity and sensitivity for microsporidian genes including those under 300 bp. Thanks to its web interface (https://microannot.org), MicroAnnot is available to the community to ensure high quality annotation of microsporidian genomes.

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Draft Genome Sequence of Tubulinosema ratisbonensis, a Microsporidian Species Infecting the Model Organism Drosophila melanogaster

Cited by 6 publications

References 22 publications

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

Identification and localization of polar tube proteins in the extruded polar tube of the microsporidian Anncaliia algerae

MicroAnnot: A Dedicated Workflow for Accurate Microsporidian Genome Annotation

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