Fly cryptochrome and the visual system

Mazzotta, Gabriella; Rossi, Alessandro; Leonardi, Emanuela; Mason, Moyra; Bertolucci, Cristiano; Caccin, Laura; Spolaore, Barbara; Martin, Alberto J. M.; Schlichting, Matthias; Grebler, Rudi; Helfrich‐Förster, Charlotte; Mammi, Stefano; Costa, Rodolfo; Tosatto, Silvio C. E.

doi:10.1073/pnas.1212317110

Cited by 62 publications

(105 citation statements)

References 49 publications

(65 reference statements)

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“…S10). Indeed, association of Cry with the scaffolding protein close to the membrane was reported recently for Drosophila (53). Such immobilization would further support Cry as a directional magnetoreceptor, as discussed earlier.…”

Section: Detection Of Directional Changes Of Mf In Cockroachesmentioning

confidence: 84%

Cryptochrome 2 mediates directional magnetoreception in cockroaches

Bazalová

Kvíćalová

Válková

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

107

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The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD•− and FADH -redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system. magnetoreception | cryptochrome | light spectrum | locomotor activity | circadian genes

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Section: Detection Of Directional Changes Of Mf In Cockroachesmentioning

confidence: 84%

Cryptochrome 2 mediates directional magnetoreception in cockroaches

Bazalová

Kvíćalová

Válková

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

107

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“…While CRY undertakes several roles in the Drosophila nervous system2956575859, recent work has shown that CRY additionally mediates acute light-dependent increases in clock cell excitability via interaction with the potassium β-subunit Hyperkinetic6061. CRY stability is light-dependent, and thus CRY protein levels increase during the night55.…”

Section: Discussionmentioning

confidence: 99%

Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila

Lamaze¹,

Öztürk-Çolak

Fischer

et al. 2017

Sci Rep

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Sleep is a highly conserved and essential behaviour in many species, including the fruit fly Drosophila melanogaster. In the wild, sensory signalling encoding environmental information must be integrated with sleep drive to ensure that sleep is not initiated during detrimental conditions. However, the molecular and circuit mechanisms by which sleep timing is modulated by the environment are unclear. Here we introduce a novel behavioural paradigm to study this issue. We show that in male fruit flies, onset of the daytime siesta is delayed by ambient temperatures above 29 °C. We term this effect Prolonged Morning Wakefulness (PMW). We show that signalling through the TrpA1 thermo-sensor is required for PMW, and that TrpA1 specifically impacts siesta onset, but not night sleep onset, in response to elevated temperatures. We identify two critical TrpA1-expressing circuits and show that both contact DN1p clock neurons, the output of which is also required for PMW. Finally, we identify the circadian blue-light photoreceptor CRYPTOCHROME as a molecular regulator of PMW, and propose a model in which the Drosophila nervous system integrates information encoding temperature, light, and time to dynamically control when sleep is initiated. Our results provide a platform to investigate how environmental inputs co-ordinately regulate sleep plasticity.

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“…The previously considered Drosophila-like-CRY sequences [4] were retrieved by Blast search using dCRY as a query. TIM sequences were collected from the same species with the same approach.…”

Section: Multiple Sequence and Structure Alignmentsmentioning

confidence: 99%

“…In the receptor region, where PLs interact with DNA, CRYs show a longer C-Terminal Tail (CTT) [3]. In spite of their structural and sequence similarity to photolyases, CRYs show a different kind of activity [4,5]. Indeed, they are involved in Insects in circadian rhythm entrainment and magnetosensitivity while in mammals they act as transcription repressors in the main negative feedback loop at the core of the clock.…”

Section: Introductionmentioning

confidence: 97%