Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila

Lamaze, Angélique; Öztürk-Çolak, Arzu; Fischer, Robin; Peschel, Nicolai; Koh, Kyunghee; Jepson, James E.C.

doi:10.1038/srep40304

Cited by 58 publications

(97 citation statements)

References 69 publications

(102 reference statements)

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“…One example of the maintenance of sleep homeostasis in respond to temperature shift is the homeostatic restoration observed in previous researches, as the differences between sleep at the treated and normal temperature in daytime are reversed at nighttime. The restoration is found when the temperature difference is as subtle as 4-5°C, but disappeared when the difference is gradually increased [7][8][9]. In our research, sleep patterns are uniformly changed under the 7°C difference and no homeostatic restoration was observed.…”

Section: Discussioncontrasting

confidence: 48%

“…Series of studies related to the effect of temperature on sleep have been reported, but the paradigms and temperature difference they implemented vary a lot [7][8][9]. We define the cold condition at 18°C, which is the lower limit of Drosophila's temperature preference [20].…”

Section: Cold Promotes Sleep In Wild Type Drosophilamentioning

confidence: 99%

“…As common as the effect is, the phenotype of sleep under different temperature vary a lot according to the duration or intensity of temperature stimuli, the difference between original and treated temperature or the rate of the transition etc. By decreasing the temperature, flies have been found either reduce their daytime sleep but prolong their nighttime sleep, or prolong their sleep both in day and night [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

Zhang

Cui

Zhu

2018

Preprint

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Sleep is regulated by environmental factors including temperature, but the neural circuits that receive the sensory signal and mediate the regulation remain unclear. We examined how cold could influence Drosophila sleep patterns and its neural mechanism. The results showed that Drosophila has more sleep duration, less sleep latency and deeper sleep depth under cold condition. We identified the Insulin-producing Cells (IPCs) can be activated by cold, and receive the cold signal from the 11216 cold-sensing neuron without a direct synaptic connection. Elevation of IPCs' sensitivity to cold impairs the sleeppromoting effect of cold while blocking of IPCs enhance the effect mostly on sleep circadian, suggesting that the cold activated IPCs have a compensative role in sleep regulation. Our finding revealed a potential neural circuit that help maintain sleep circadian in detrimental environment and may give new insight to the complicated sleep regulation mechanism.

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Section: Discussioncontrasting

confidence: 48%

Section: Cold Promotes Sleep In Wild Type Drosophilamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

Zhang

Cui

Zhu

2018

Preprint

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“…Using the standard definition of a Drosophila sleep bout as a 5 min period of inactivity (measured using the Drosophila Activity Monitor (DAM) system [6]), we recently showed that elevating ambient temperature from 22°C to ≥ 30°C delays onset of the first morning sleep bout in Drosophila males [3]. However, Drosophila sleep is often initially fragmented during the early morning at lowto-medium temperatures ( Figure 1A).…”

Section: Consolidated Sleep Onset In Drosophila Is Gated By Circadianmentioning

confidence: 99%

A sleep-regulatory circuit integrating circadian, homeostatic and environmental information in Drosophila

Lamaze¹,

Krätschmer²,

Jepson³

2018

Preprint

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SUMMARYIn the wild, when to go to sleep is a critical decision. Sleep onset is controlled by two processes: the circadian clock, and a homeostat measuring sleep drive [1,2]. Environmental stimuli must also clearly intersect with the circadian clock and/or homeostat so that sleep is initiated only when appropriate. Yet how circadian, homeostatic and environmental cues are integrated at the circuit level is unclear. Recently, we found that DN1p clock neurons in Drosophila act to prolong morning wakefulness at elevated ambient temperatures [3]. Here we show that a subset of DN1p neurons exhibit temperature-sensitive increases in excitability, and define an output pathway linking DN1p neurons to downstream sleep-regulatory circuits. We show that DN1p neurons project axons to a subdomain of the Anterior Optic Tubercle (AOTU), and here make inhibitory synaptic connections with sleep-promoting tubercular-bulbar (TuBu) neurons. Using unbiased trans-synaptic labeling, we show that these TuBu neurons form synaptic connections with R-neurons innervating the ellipsoid body, subsets of which control homeostatic sleep drive [4]. DN1p excitability is clock-dependent, peaking in the late night and . CC-BY-NC-ND 4.0 International license not peer-reviewed) is the author/funder. It is made available under aThe copyright holder for this preprint (which was . http://dx.doi.org/10.1101/250829 doi: bioRxiv preprint first posted online Jan. 22, 2018; 2 early morning [5]. Thus, integration of circadian and thermo-sensory information by DN1p neurons and subsequent inhibition of sleep-promoting TuBu neurons provides a mechanism by which an environmental stimulus can regulate sleep onset during a specific compartment of the day-night cycle.Furthermore, our results suggest that the AOTU functionally links circadian and sleep homeostat circuits in Drosophila.. CC-BY-NC-ND 4.0 International license not peer-reviewed) is the author/funder. It is made available under aThe copyright holder for this preprint (which was . http://dx.doi.org/10.1101/250829 doi: bioRxiv preprint first posted online Jan. 22, 2018; 3 RESULTS AND DISCUSSION Consolidated sleep onset in Drosophila is gated by circadian and thermal cuesUsing the standard definition of a Drosophila sleep bout as a 5 min period of inactivity (measured using the Drosophila Activity Monitor (DAM) system [6]), we recently showed that elevating ambient temperature from 22°C to ≥ 30°C delays onset of the first morning sleep bout in Drosophila males [3]. However, Drosophila sleep is often initially fragmented during the early morning at lowto-medium temperatures ( Figure 1A). To gain a more robust view of how temperature changes affect consolidated sleep, we generated an R-based program capable of quantifying up to 30 distinct sleep parameters using raw DAM system data (see STAR Methods). We used this program to analyse how various properties of sleep architecture were modulated by temperature increases (Figure S1A-J), particularly the daytime longest sleep bout (dLSB).Given the prolonged i...

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“…This led to our second problem, finding confounding phenotypes from mechanical agitation resulting from the movement. Third, published methods for changing temperature are often slow [5,12].…”

Section: Introductionmentioning

confidence: 99%

An inexpensive air stream temperature controller and its use to facilitate temperature controlled behavior in livingDrosophila

Sangston

Hirsh

2018

Preprint

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Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster.Utilizing flies expressing the temperature inducible channel dTrpA1 in dopaminergic neurons, we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature modulated biological reagents. Method Summary:We develop widely applicable and affordable solution to rapidly changing temperature within an enclosed chamber using commercially available components.Funding Acknowledgements:

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Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila

Cited by 58 publications

References 69 publications

Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

Insulin Producing Cells Monitor the Cold and Compensate the Cold-Induced Sleep in Drosophila

A sleep-regulatory circuit integrating circadian, homeostatic and environmental information in Drosophila

An inexpensive air stream temperature controller and its use to facilitate temperature controlled behavior in livingDrosophila

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