Flux control analysis of a lactate and sucrose metabolic network at different storage temperatures for Hami melon (Cucumis melo var. saccharinus)

Cai, Xiuzhen; Wang, Huisong; Pang, Guangchang

doi:10.1016/j.scienta.2014.10.040

Cited by 20 publications

(8 citation statements)

References 34 publications

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“…With increasing interest in its biological properties and economic importance, the oriental melon has become an attractive model for studying valuable features, such as fruit morphology (Saladie et al, 2015), tissue specific RNA-seq (Cai, Wang & Pang, 2015; Cao et al, 2016; Kim et al, 2016b; Liu et al, 2016), pathogen resistance (Bezirganoglu et al, 2013), and understand the specialization of some genes involved in plant growth (Cai, Wang & Pang, 2015; Cao et al, 2016; Kim et al, 2016b; Liu et al, 2016). Despite these studies, the molecular mechanisms of fruit development in the oriental melon are still not elucidated.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development

Shin

Kim

Koo

et al. 2017

PeerJ

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BackgroundThe oriental melon (Cucumis melo L. var. makuwa) is one of the most important cultivated cucurbits grown widely in Korea, Japan, and northern China. It is cultivated because its fruit has a sweet aromatic flavor and is rich in soluble sugars, organic acids, minerals, and vitamins. In order to elucidate the genetic and molecular basis of the developmental changes that determine size, color, and sugar contents of the fruit, we performed de novo transcriptome sequencing to analyze the genes expressed during fruit development.ResultsWe identified a total of 47,666 of representative loci from 100,875 transcripts and functionally annotated 33,963 of the loci based on orthologs in Arabidopsis thaliana. Among those loci, we identified 5,173 differentially expressed genes, which were classified into 14 clusters base on the modulation of their expression patterns. The expression patterns suggested that the differentially expressed genes were related to fruit development and maturation through diverse metabolic pathways. Analyses based on gene set enrichment and the pathways described in the Kyoto Encyclopedia of Genes and Genomes suggested that the expression of genes involved in starch and sucrose metabolism and carotenoid biosynthesis were regulated dynamically during fruit development and subsequent maturation.ConclusionOur results provide the gene expression patterns related to different stages of fruit development and maturation in the oriental melon. The expression patterns give clues about important regulatory mechanisms, especially those involving starch, sugar, and carotenoid biosynthesis, in the development of the oriental melon fruit.

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development

Shin

Kim

Koo

et al. 2017

PeerJ

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“…4). In Hami melons, fruit at 5°C has a higher sucrose synthesis flux than fruit at 0°C (Cai et al 2015). However, peach fruit at 5°C exhibits a sharp decrease in sucrose content and an increase in reducing sugars ).…”

Section: Changes In Activities Of Sugar Metabolism Enzymesmentioning

confidence: 95%

Changes in soluble sugar metabolism in loquat fruit during different cold storage

Wei

Shao

2017

J Food Sci Technol

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To investigate soluble sugar metabolism and its relationship to chilling injury (CI), loquat fruits were stored at 0 and 5°C for 5 weeks. CI symptoms were monitored during storage, and soluble sugar content and related enzyme activities were measured. Enzymes activities and substrate levels in the ascorbate-glutathione (AsA-GSH) cycle were also determined. Fruit at 0°C had lower sucrose levels and higher levels of glucose and fructose than fruit maintained at 5°C, and also manifested less severe CI symptoms. Fruit at 0°C also exhibited increased activities of acid invertase, neutral invertase, sucrose phosphatesynthase and sucrose synthase. AsA-GSH cycle activity at 0°C was much higher than that at 5°C. Glucose levels correlated positively with levels of AsA and GSH and ascorbate peroxidase (APX) activity. The increased glucose levels are sufficient to account for the chilling tolerance of loquat fruit.

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“…Deng, Pan, Chen, Shen, and Sheng (2013) confirmed that SPS and NI were relatively key enzymes in "Golden Delicious" apple on account of a steady less level of AI during the entire storage. Cai, Wang, and Pang (2015) showed that SPS played an important role in controlling sucrose flux through metabolic pathways. Itai and Tanahashi reported that the accumulation of SPS/1 transcript contributed to the inhibition of sucrose loss in fruit during cold storage and SPS is the key enzyme involved in sucrose synthesis (Itai & Tanahashi, 2008…”

Section: Activities Of Related Enzymes With Sucrose Metabolismmentioning

confidence: 99%

Changes in nutrition and related enzymes of Annona squamosa during storage based on carbohydrate analysis

Ren

Dong

Wang

et al. 2019

J Food Process Preserv

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Firmness and soluble solids content in Annona squamosa during storage were studied. Changes in total Annona squamosa polysaccharides (TASP) obtained by alcohol precipitation and corresponding ethanol supernatants (TASP‐S) during storage were analyzed. Ethanol supernatants from Annona squamosa stored for 3 days (TASP‐S3) were chosen and small molecule carbohydrate (TASP‐S3‐1) in it was characterized in structure. Activities of related enzyme during storage were determined. Results indicated firmness exhibited a significant decline from 1 day to 3 days and soluble solids content fluctuated during storage. With prolonged storage time, relative content of each component in TASP varied and that of carbohydrate with higher molecular weight gradually decreased. So was the compositions in TASP‐S during storage. TASP‐S3‐1 was ultimately identified to be saccharose. Enzyme assays indicated activities of related enzymes varied during storage, and acid invertase, sucrose synthase might play more important roles in improving the quality and nutrition of postharvest Annona squamosa. Practical applications This work lay the foundation for further studying carbohydrate metabolism and controlling the quality of postharvest Annona squamosa. Because the relative contents of each components in TASP and TASP‐S, changed with the extension of storage time. Besides, among these related enzymes, acid invertase and sucrose synthase would be more important in postharvest Annona squamosa, which provided critical information on improving the quality and nutrition of Annona squamosa in further study.

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Flux control analysis of a lactate and sucrose metabolic network at different storage temperatures for Hami melon (Cucumis melo var. saccharinus)

Cited by 20 publications

References 34 publications

Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development

Transcriptome analysis of the oriental melon (Cucumis meloL. var.makuwa) during fruit development

Changes in soluble sugar metabolism in loquat fruit during different cold storage

Changes in nutrition and related enzymes of Annona squamosa during storage based on carbohydrate analysis

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