Flurochloridone-based herbicides induced genotoxicity effects on Rhinella arenarum tadpoles (Anura: Bufonidae)

Nikoloff, Noelia; Natale, Guillermo Sebastián; Marino, Damián; Soloneski, Sonia; Larramendy, Marcelo L.

doi:10.1016/j.ecoenv.2013.10.021

Cited by 45 publications

(53 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The MN analysis is used worldwide as a bioassay of genotoxicity to detect small chromosomal fragments induced by clastogens or vagrant chromosomes produced by aneugens (OECD, 2007). The induction of MNs in circulating erythrocytes from different amphibian tadpole species as a consequence of pesticide treatments is well documented (Bouhafs et al, 2009;Nikoloff et al, 2014b;Vera Candioti et al, 2010). Among them, H. pulchellus has been used previously as an experimental model in genotoxicity studies.…”

Section: Figmentioning

confidence: 97%

“…Whereas GS25 tadpoles were exposed to 0.27, 0.41, 0.54, 0.68, 0.81, 0.87, 0.95, 1.02, 1.09, 1.22, and 2.72 mg/L IMZT, GS36 tadpoles were exposed to 0.41, 0.81, 1.09, 1.22, 1.36, 1.49, 1.63, 2.17, and 2.72 mg/L IMZT. Whereas the negative control group consisted of five organisms kept in dechlorinated tap water, the positive control group consisted of five tadpoles treated with 23 mg/L Cr(VI) as reported previously (Nikoloff et al, 2014b;Vera Candioti et al, 2010).…”

Section: Experimental Designmentioning

confidence: 99%

“…To accomplish this target, several sublethal end points for testing both genotoxicity and cytotoxicity have been employed on aquatic organisms, including amphibians. Among them, analysis of micronucleus (MN) frequency and the induction of DNA single-strand breaks by the single cell gel electrophoresis (SCGE) assay are the most frequently used and recommended end points for detecting cytogenetic and primary DNA damage in circulating nucleated cells, respectively (Hartmann et al, 2003;Nikoloff et al, 2014b;Pérez-Iglesias et al, 2014;Vera Candioti et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias

Soloneski

Nikoloff

et al. 2015

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

Section: Figmentioning

confidence: 97%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias

Soloneski

Nikoloff

et al. 2015

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

“…Although we could not measure the amount of pesticides and heavy metals, according to the local residents, those areas are well known for the use of agrochemicals, so the greater extent of DNA damage in the animals collected from perturbed areas can be explained by their characteristics, such as the presence of agrochemicals. Several authors have reported that chronic and/or acute exposure to heavy metals, herbicides, and pesticides can result in a variety of genotoxic, mutagenic, teratogenetic, and reproductive anomalies in amphibians (Campana et al 2003;Unrine et al 2007;Yin et al 2008;Peltzer et al 2008;Liu et al 2011;Nikoloff et al 2014). However, due to the complex mix of substances in agroecosystems, which could not be measured, it is difficult to link the genotoxic effects to a specific agent or element (Govindarajulu 2008).…”

Section: Discussionmentioning

confidence: 99%

Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

Gonçalves

Vieira

Maciel

et al. 2014

Environ Sci Pollut Res

View full text Add to dashboard Cite

The aim of the study was to use the comet assay (single-cell gel electrophoresis) and micronucleus test to assess the extent of genomic damage in the whole blood of Dendropsophus minutus from agroecosystems with great use of agrochemicals and to compare the results to those obtained from animals living in unpolluted areas. Our results indicated that specimens of D. minutus collected in perturbed areas exhibited higher amounts of DNA damage in blood cells in comparison to animals from areas free of agricultural activities. The average and standard deviation of all comet assay parameters (tail length, percentage of DNA in the tail, and olive tail moment) and micronuclei frequency were significantly higher in specimens collected in perturbed areas than in the animals from preserved areas. Our study showed that animals from perturbed areas, such as agroecosystems, tend to have higher amounts of DNA damage than animals from reference areas. Moreover, we can conclude that D. minutus tadpoles could be included as a model organism in biomonitoring studies.

show abstract

“…On the other hand, diverse studies about genotoxic and/ or cytotoxic effects obtained with pure and commercial formulations of diverse pesticides indicate that commercial formulations may contain additional unsafe xenobiotics supporting the importance of evaluating not only the active principle but also the commercial formulation, which in fact constitutes the real hazard from agrochemicals (42)(43)(44)(45)(46)(47)(48)(49)(50)(51).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

This study evaluated direct and metabolic genotoxic effects caused by Lannate-90 ® , a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L ® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90 ® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90 ® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90 ® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.

show abstract

Flurochloridone-based herbicides induced genotoxicity effects on Rhinella arenarum tadpoles (Anura: Bufonidae)

Cited by 45 publications

References 51 publications

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Contact Info

Product

Resources

About