Fluorous Solid-Phase Extraction Technique Based on Nanographite Fluoride

Abstract

Fluorous solid-phase extraction (FSPE) has been employed to isolate target compounds from complex chemical or biological samples in many research areas. However, the lack of efficient and economical available perfluorinated materials impeded its development. In this study, we present a novel nanographite fluoride-based fluorous solid-phase extraction (GF-FSPE) as a replacement of commercially available cartridge-based FSPE, which showed remarkable selectivity, low LOD, high post enrichment recovery, and large enrichment capacity. To demonstrate the potency of GF-FSPE, it was designed and successfully applied to isolate cysteine-containing peptide subsets from complex protein samples to improve the proteome coverage. Additionally, since graphite fluoride was inexpensive and highly commercialized, this study was expected to promote the popularization of FSPE in both chemical and biological separations as well as encourage the synthesis and broaden the application of highly fluorinated carbon fluoride in material science.

FluoroTRAQ: Quantitative Analysis of Protein S-Nitrosylation through Fluorous Solid-Phase Extraction Combining with iTRAQ by Mass Spectrometry

“…20 The fluorous tag can also promote the ionization of analytes during the MS process due to the hydrophobicity of the fluorous portion, which makes FSPE suitable for the analysis of analytes of low abundance and low ionization efficiency. 21,22 The technical feature of FSPE thereby makes it a powerful tool for the proteome-wide investigation of protein PTMs, which has already been reported by several research groups including ours. 22−24 In this work, based on the idea of FSPE, an analytical strategy for the dynamic and site-specific quantitative analysis of SNOproteome was developed.…”

Application of nanomaterials in proteomics-driven precision medicine

“…Subsequently, the fluorescence-tagged SNO-peptides were captured by nanographite fluoride specifically through fluorous-fluorous interactions. Taking advantage of the highly fluorinated level and the high surface area of nanographite fluoride, the enrichment approach was shown to have remarkable selectivity, good sensitivity, high post-enrichment recovery, and large enrichment capacity 42 , 43 .…”

Discover the Post‐Translational Modification Proteome Using Mass Spectrometry

“…To further increase the efficiency of fluorous solid phase extraction (FSPE), we investigated a novel nanographite fluoride as a replacement of commercially available cartridge‐based FSPE. [ 25 ] With the higher surface area and more abundant sites for immobilizing the fluorine, the nanographite fluoride had many advantages over the commercially available FSPE cartridge including better selectivity, higher post enrichment recovery and larger enrichment capacity, which facilitated more sensitive detection and quantitation of N‐glycans via mass spectrometry. [ 26 ] In addition, to profile the N‐glycome from serum exosomes, which need high analytical sensitivity due to the trace amount of exosomes, we used reverse capture strategy to enrich and purify the N‐glycans.…”