Fluoroquinolone-Resistant Escherichia coli and Proteus mirabilis in Poultry of Middle Moravia, Czech Republic

Abstract

The aim of the study was to detect antibiotic resistance of Escherichia coli and Proteus mirabilis isolates from farm-reared poultry.During the period of June 2003 to June 2004, samples of cloacal swabs obtained from 5 poultry farms located in the central part of Moravia, Czech Republic were examined using aerobic cultivation and afterwards E. coli and P. mirabilis isolates were identified. Minimal inhibitory concentrations of antibiotics tested were determined by a microdilution method. Pulsed-field gel electrophoresis (PFGE) was performed using SmaI restriction endonuclease.A total of 300 samples of cloacal swabs from healthy layer hens were cultivated and 239 E. coli and 127 P. mirabilis strains were isolated. In the case of E. coli, 7 isolates (3%) from 4 different farms were identified manifesting resistance to ofloxacin and ciprofloxacin. Out of 24 P. mirabilis isolates resistant to the tested fluoroquinolones, 20 strains were isolated from one of the farms and PFGE analysis of DNA proved that 19 isolates were probably identical and represented one clonal type.The study confirmed the occurrence of multiresistant bacterial isolates with resistance to fluoroquinolones in poultry in the Czech Republic. Their clonal spread in farm-reared poultry can be suggested, too.

Changes in Concentrations of Fluoroquinolones and of Ciprofloxacin-resistant Enterobacteriaceae in Chicken Feces and Manure Stored in a Heap

“…Th ese two genera, which are widespread in the environment (O'Hara et al, 2000), thus appear to be more adapted than E. coli to the conditions found in the heaps. Th e presence of resistant Proteus in feces and in chicken manure in our study is in accordance with the results of Kolar et al (2005), who detected Proteus mirabilis resistant to CIP in the cloacae of hens. As observed for E. coli, resistant isolates of P. mirabilis were present in both control and treated chickens.…”

Upending a ‘Totality’: Re-evaluating Corded Ware Variability in Late Neolithic Europe

“…There are only a handful of real single graves from the typical barrows on the Danish Isles (Ebbesen 2006; Jacobs 1991). In the Krakow area of Lesser Poland and in Moravia, north–south and north-east–south-west orientation prevails (Włodarczak 2006; Šebela 1999; Kolář 2011). In the Lubaczów-group of south-eastern Poland (Machnik 1966), in Kujawia (Czebreszuk 1996) and Hesse, Germany (Wiermann 2004), neither the dominance of west–east orientation nor a gender differentiation can be proven statistically. In the area of the Baltic States, the few Corded Ware graves that exist show neither a predominantly west–east orientation, nor gender differentiation (Kilian 1955, 64). In the southern German Tauber valley, there is no gender differentiation detectable in the orientation: west–east prevails, as do collective burials (Dornheim et al .…”

“…In the Krakow area of Lesser Poland and in Moravia, north–south and north-east–south-west orientation prevails (Włodarczak 2006; Šebela 1999; Kolář 2011).…”

Quinolone-resistant Escherichia coli in Poultry Farming

“…In veterinary medicine a large proportion of these antimicrobials belong to a group of drugs with the so-called restricted indication. It means that they may be used only if the causative bacterial agent is identified and tested for susceptibility to various antibiotics, with the results clearly showing that for the particular purpose no other suitable agent without such a restriction is available (5,6).…”