Fluorometric Riboflavin Titration in Plasma by Ribof lavin&lt;sub&gt;-&lt;/sub&gt;Binding Apoprotein as a Method for Vitamin B&lt;sub&gt;2&lt;/sub&gt; Status Assessment

Коденцова, В М; Vrzhesinskaya, O.A.; Спиричев, В. Б.

doi:10.1159/000177885

Cited by 11 publications

(6 citation statements)

References 5 publications

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“…Official methods for quantification of Rf in food samples are based usually on HPLC measurement and microbiological assays. Alternative methods involve electrochemical or fluorescent characteristics of flavins for standard solutions, [1][2][3] biological fluids, 4,5 and pharmaceutical tablets 6 but none for food matrixes. In addition to these approaches, methods based on biological properties of Rf through the binding with Rf binding protein (RBP) from egg white [7][8][9] have been investigated.…”

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confidence: 99%

Biosensor-Based Determination of Riboflavin in Milk Samples

2003

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An assay for quantification of riboflavin (Rf) in milk-based products has been developed using the principle of surface plasmon resonance with on-chip measurement. The quantification was done indirectly by measuring excess of Rf binding protein (RBP) that remains free after complexation with Rf molecules originally present in the sample solution. The chip was modified with covalently immobilized Rf in order to bind the RBP in excess. A chemical modification was performed to introduce a reactive ester group at the N-3 position of the natural Rf to bind amino groups present on the chip surface. Calibration solutions were prepared by mixing a range of Rf standard solutions with an optimized concentration of RBP. The Rf content in the milk-based products was then measured by comparison of the response against the calibration. Results obtained were very close to those from an official HPLC-fluorescence procedure. The limit of quantification was determined to 234 microg/L and the limit of detection to 70 microg/L by an injection volume of 160 microL.

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confidence: 99%

Biosensor-Based Determination of Riboflavin in Milk Samples

2003

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“…Vitamin status was assessed by the content of vitamins in the serum. The concentration of retinol (vitamin A) and betacarotene was determined using high performance liquid chromatography [12], riboflavin (vitamin B 2 )by fluorimetric method using riboflavin-binding apoprotein [13], 25-hydroxyvitamin D [25(OH)D] (vitamin D)enzyme immunoassay method using the test system «ELECSYS Vitamin D Total» (F. Hoffmann-La Roche Ltd., Switzerland). Persons with indicators that did not reach the lower limit of the norm [13] were considered to be insufficiently provided with vitamins.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of vitamin status of the population of the russian arctic according to the data on vitamin consumption and serum level

Beketova

Vrzhesinskaya

Keshabyants

et al. 2019

I.P. Pavlov Russian Medical Biological Herald

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Aim. To compare the calculated data of A, B2, D vitamins and beta-carotene consumption with diets and biomarkers of vitamin status of 178 residents of Tazovsky and Gyda settlements of Yamal-Nenets Autonomous Area. Materials and Methods. The actual nutrition was studied by frequency (according to the frequency of food consumption for the previous month) and 24-hour (daily) reproduction methods. Vitamin status was assessed by serum vitamin concentration. Results. A statistically significant relationship between serum beta-carotene level and its consumption with rations was established. There was no significant relationship between the level of fish consumption and vitamin D sufficiency. Conclusion. Vitamin intake data provide a framework to reveal deviations from optimal nutrition and to identify risk groups with vitamin deficiency, but they do not always make it possible to carry out an objective assessment of individual vitamin sufficiency. Thus, in individuals with a clear lack of vitamin A in the diet on the previous day before taking blood, blood retinol level was in the normal range.

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“…Концентрацию витаминов А (ретинола) и Е (α-, γ-токоферол и их сумма), β-каротина определяли с помощью обращенно-фазовой высокоэффективной жидкостной хроматографии [14], В 2 (рибофлавина) -флуориметрически с использованием рибофлавинсвязывающего апобелка [15], С (аскорбиновая кислота) -титрованием реактивом Тильманса [16]. В качестве критериев обеспеченности витаминами традиционно использовали величины, обоснованные в предыдущих исследованиях [17], составившие для витамина А 30-80 мкг/дл, суммы токоферолов -0,8-1,5 мг/дл, рибофлавина -5-20 нг/мл, аскорбиновой кислоты -0,4-1,5 мг/дл, β-каротина ->10 мкг/дл.…”

Section: методы регистрации исходовunclassified

Vitamin status of patients with certain chronic non-communicable diseases

Sharafetdinov

Хамзярович²,

Коденцова

et al. 2020

Clinical nutrition and metabolism

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Background. Inadequate supply with vitamins is a risk factor for the development of many nutritionally-related diseases and their progression. Data on the actual vitamin status of patients are necessary to develop measures for its improvement. Aim. To characterize the supply of persons with non-communicable diseases with vitamins A, E, C, B2 and -carotene by determining their level in the blood of patients. Material and methods. The blood serum level of vitamins C, A, E, B2 and -carotene in 138 patients (41 men and 97 women) 2280 years old with cardiovascular diseases, obesity, gastrointestinal diseases, type 2 diabetes mellitus (T2DM), osteoarthrosis has been determined. Results. Vitamin C concentration corresponded to adequate status in approximately 2/3 of the examined patients; among patients with T2DM, such sufficiency occurred 1.61.9 fold less often than in other groups. The frequency of reduced levels of vitamins C, A and E was statistically significantly more frequent in patients with gastrointestinal diseases. The -/-tocopherol ratio in the serum of patients in all groups was close to 1:50, while in patients with gastrointestinal diseases reached 1:60.7. The proportion of patients sufficiently supplied with all studied vitamins ranged from 15.8 to 70.0%. Patients with osteoarthrosis were best of all provided with all vitamins: multiple (3 or more vitamins) vitamin deficiency was not found. In other groups of patients, multiple vitamin deficiency occurred in 5.327.6% of the examined (an average of 16.4%). Among patients with gastrointestinal diseases there was not a single person sufficiently provided with all the studied vitamins. Multiple vitamin deficiency in patients with gastrointestinal diseases was detected more often (p 0.01) compared with patients with T2DM and osteoarthrosis. Given the high prevalence of vitamin D deficiency, it is possible to extrapolate that a significant proportion of patients with a combined deficiency of 2 vitamins (6.931.6% in the samples examined) will move into the category of persons with a simultaneous deficiency of 3 vitamins. Conclusion. The purposeful development of supplements containing effective doses of vitamins for various nosologies is necessary.

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Fluorometric Riboflavin Titration in Plasma by Ribof lavin<sub>-</sub>Binding Apoprotein as a Method for Vitamin B<sub>2</sub> Status Assessment

Cited by 11 publications

References 5 publications

Biosensor-Based Determination of Riboflavin in Milk Samples

Biosensor-Based Determination of Riboflavin in Milk Samples

Evaluation of vitamin status of the population of the russian arctic according to the data on vitamin consumption and serum level

Vitamin status of patients with certain chronic non-communicable diseases

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