Fluorometric paired emitter detector diode (FPEDD)

Tymecki, Łukasz; Pokrzywnicka, Marta; Koncki, Robert

doi:10.1039/c0an00227e

Cited by 22 publications

(9 citation statements)

References 21 publications

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“…Koncki et al reported the use of LEDs for both absorption and fluorescence measurement. 25,26 The PEDD has been employed in the analysis of physiological samples, [27][28][29][30][31][32][33][34][35][36][37][38][39][40] drink and food samples [41][42][43][44] and environmental samples. 45 There are also reports of dual analysis employing PEDDs, for example, multicommutated flow (MCFA) system for the determination of activity of acid phosphate and alkaline phosphatase, 46 sequential injection (SI) system for quantitative measurement of sucrose and phosphate, 47 MCFA system for determination of phosphate and calcium, 48 MCFA system for total protein and creatinine analysis 49 and MCFA system for investigation of iron-transferrin and apotransferrin speciation in human serum.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

et al. 2019

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An effective flow injection analysis (FIA) system employing paired emitter detector diode (PEDD) for simultaneous dual antioxidant assays is proposed. The total antioxidant capacity (TAC) was measured using the 2,2′-azino-bis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay in parallel with the ferric reducing antioxidant power (FRAP) assay. Ascorbic acid was used as the reference antioxidant and the TAC value reported as ascorbic acid equivalent (AAE). A 10port valve furnished with two injection loops allowed the sample from one loop to react with the ABTS reagent and be detected by one PEDD. At the same time, the sample from the second loop reacted with the FRAP reagent and is detected at the second PEDD. A pair of red light emitting diodes (634 nm) was employed for both PEDDs. The linearity range was 10 to 50 μM ascorbic acid, with limit of detection of 3.18 and 4.37 μM, and precision of 2.41 and 2.15% RSD (for 30 μM ascorbic acid, n = 10) for the ABTS and FRAP assay, respectively. Sample throughput of 90 samples/h was achieved. The method was applied to the measurement of TAC of commercial fruit juice, instant tea products and vitamin C tablets. The observed AAE values were in good agreement with those obtained using batch methods with a spectrophotometer.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

et al. 2019

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“…3,4,17,18 The use of the LED in photovoltaic mode has also been reported. 14,34,35 An LED is a current source; the current generated is in proportion to the incident radiation. When the current (I) flows through a resistor (R), the developed voltage (V) across the resistor is in proportion to the current, through Ohm's law: V = RI.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Self-constructed and relatively simple fluorescence instruments have been reported for more than 50 years. − However, it is only since about the year 2000 that LEDs have been used (as light sources), making the instruments both cheaper and simpler. − Photometers using an LED as a light detector generally use the LED in reverse biased mode, requiring an external voltage and some additional circuitry. ,,, The use of the LED in photovoltaic mode has also been reported. ,, An LED is a current source; the current generated is in proportion to the incident radiation. When the current ( I ) flows through a resistor ( R ), the developed voltage ( V ) across the resistor is in proportion to the current, through Ohm’s law: V = RI .…”

Section: Introductionmentioning

confidence: 99%

Demonstrating Basic Properties of Spectroscopy Using a Self-Constructed Combined Fluorimeter and UV-Photometer

Kvittingen¹,

Kvittingen

Melø

et al. 2017

J. Chem. Educ.

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This article describes a combined UV-photometer and fluorimeter constructed from 3 LEDs and a few wires, all held in place with Lego bricks. The instrument has a flexible design. In its simplest version, two UV-LEDs (355 nm) are used as light source and to detect absorption, and a third LED, in the visible spectrum (e.g., 525 nm), is used to detect fluorescence. Various experiments are described: fluorescence as a function of concentration and light source intensity; quantitative measurements of quinine in tonic water, using absorption and fluorescence measurements, with results in line with those obtained on scientific instruments; a demonstration of the inner filter effect reducing fluorescence at higher concentrations; investigation of the effect of drinking tonic water on the fluorescence of quinine in urine; temperature effects on fluorescence; chloride-ion quenching of fluorescence with result comparable to those previously reported; and, finally, a demonstration of phosphorescence.

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“…Our research group has demonstrated that PEDDs can be also configured as complete and effective fluorimetric detectors consisting of integrated LED inductor and LED detector of fluorescence [ 17 , 25 ]. Such approach is possible because LEDs are found to be partially selective detectors able to detect fluorescence emission with negligible effects from the exciting light.…”

Section: Introductionmentioning

confidence: 99%

“…Such approach is possible because LEDs are found to be partially selective detectors able to detect fluorescence emission with negligible effects from the exciting light. Until now, two and three LED-based fluorimetric detectors have been successfully developed for determination of calcium [ 25 , 26 ] and phosphate [ 14 , 15 ] ions, quinine [ 17 ], vitamin B [ 27 ], and proteins [ 20 ]. Only recently, turbidimetric [ 28 – 31 ] and nephelometric [ 30 , 31 ] detectors operating according to PEDD principle have been reported.…”

Section: Introductionmentioning

confidence: 99%

Towards optoelectronic urea biosensors

2015

Self Cite

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Integration of immobilized enzymes with light-emitting diodes (LEDs) leads to the development of optoelectronic enzyme-based biosensors. In this work, urease, used as a model enzyme, immobilized in the form of an open-tubular microbioreactor or biosensing membrane that has been integrated with two red LEDs. It forms complete, fiberless, miniaturized, and extremely economic biooptoelectronic devices useful for nonstationary measurements under flow analysis conditions. Both enzyme-based biodevices, operating according to the paired emitter detector diode (PEDD) principle, allow relatively fast, highly sensitive, and well-reproducible urea detection in the millimolar range of concentrations. Potential analytical applications of the developed urea bioPEDDs have been announced. Both presented constructions will be easily adapted for the development of other optoelectronic biosensors exploring various enzyme-based schemes of biodetection.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-014-8434-z) contains supplementary material, which is available to authorized users.

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Fluorometric paired emitter detector diode (FPEDD)

Abstract: Integration of two light emitting diodes allows construction of a fluorometric paired emitter detector diode (FPEDD)--a compact optoelectronic device useful as a complete flow-through fluorescence detector.

Cited by 22 publications

References 21 publications

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

Simultaneous Colorimetric Measurements of Antioxidant Capacity by Flow Injection Analysis with Paired Emitter Detector Diode

Demonstrating Basic Properties of Spectroscopy Using a Self-Constructed Combined Fluorimeter and UV-Photometer

Towards optoelectronic urea biosensors

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