Fluorometric determination of okadaic acid using a truncated aptamer

Chinnappan, Raja; AlZabn, Razan; Mir, Tanveer Ahmad; Bader, Mamoun M.; Zourob, Mohammed

doi:10.1007/s00604-019-3517-3

Cited by 31 publications

(14 citation statements)

References 34 publications

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“…Both fluorescently-labeled and label-free biosensing approaches have been reported with the possibility of on-site detection using portable analyzers [ 56 , 57 , 58 , 59 ]. The target of interest can range from small molecules such as the mycotoxins aflatoxin B1 [ 60 ] and ochratoxin A [ 61 ], bisphenol A [ 62 ], the antibiotic tobramycin [ 54 ] and the marine toxin okadaic acid [ 63 ], to larger entities such as lipopolysaccharides [ 64 ] and the cyclic heptapeptide microcystin-LR (a hepatotoxin produced by cyanobacteria) [ 65 ]. Furthermore, proteins such as tropomyosin (a major shrimp allergen) [ 66 ] and glycated hemoglobin [ 67 ] or even whole bacteria such as Salmonella enteritidis [ 68 ] can be measured using this approach.…”

Section: Strategies For Improving Binding Affinity Through Chemicamentioning

confidence: 99%

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Elskens

Madder

2020

IJMS

110

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Aptamers are short single stranded DNA or RNA oligonucleotides that can recognize analytes with extraordinary target selectivity and affinity. Despite their promising properties and diagnostic potential, the number of commercial applications remains scarce. In order to endow them with novel recognition motifs and enhanced properties, chemical modification of aptamers has been pursued. This review focuses on chemical modifications, aimed at increasing the binding affinity for the aptamer’s target either in a non-covalent or covalent fashion, hereby improving their application potential in a diagnostic context. An overview of current methodologies will be given, thereby distinguishing between pre- and post-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) modifications.

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Section: Strategies For Improving Binding Affinity Through Chemicamentioning

confidence: 99%

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Elskens

Madder

2020

IJMS

110

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“…51 G-quadruplexes scoring of the N59af (the reverse complement of N59) revealed that N59af had the potential to form several kinds of Gquadruplexes structures, and N59a itself had the potential to form an i-motif fold structure by itself theoretically. 52,53 To verify these, we disrupted the arrangement mode of the bases. Therefore, N59a was truncated to obtain N59a2, N59a3, N59a4, N59a5, N59a6, N59a7 (Table S4 †).…”

Section: Identication Of Affinity and Specicity Of Aptamer N59amentioning

confidence: 99%

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

Zhou

et al. 2020

RSC Adv.

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“…For example, Shi et al developed an SQX-specific aptamer after two truncations [ 4 ]. Although a new aptamer with a higher affinity was obtained, the method was cumbersome and required multiple cuts [ 4 , 17 ]. Therefore, an efficient and straightforward aptamers truncation approach is required.…”

Section: Introductionmentioning

confidence: 99%

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water

et al. 2022

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Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (Kd = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25–160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1–100.1% and coefficients of variation (CVs) of 2.2–10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.

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Fluorometric determination of okadaic acid using a truncated aptamer

Cited by 31 publications

References 34 publications

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water

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