Fluorometric determination of microgram amounts of meperidine

Abstract

very lean flame, rapid closure of the fuel valve produced no flashback. However, if the flame is left running for any length of time under these reduced flow conditions, localized overheating may cause damage to the burner.The 50-mm long-path nitrous oxide-acetylene flame shows a very stable CN emission region for some 20 mm above the burner head. Inert gas sheathing again extends the size and shape of this reducing region.The stability and intensity of the CN emission was measured just above the primary reaction cones with the circular burner. The results with and without nitrogen separation are shown in Figure 2 in comparison with a typical "circular slot" burner (2).Emission intensities for a restricted range of elements, from those which are readily atomized (e.g., sodium) to those which form refractory oxides (e.g., aluminium), were compared for the circular capillary and the circular slot burners operated both with and without flame separation (Table II). Although both the burner heads were of the same diameter and the gas flows were equivalent, the circular capillary burner gives a greater emission signal in all instances. Burner heads may be readily constructed from stainless steel capillary tubing on which stable laminar flames may be maintained. These burners are safer in operation than conventional burners, even with the nitrous oxide-acetylene flame. The method of construction may be used for flame or inert gas shields for these burners.

Fluorometric analysis

“…A method for methadone was based on condensation with formaldehyde in sulfuric acid, yielding a fluorophore with 270 nm excitation peak and 450 nm emission peak (596). Meperidine interferes because it undergoes a similar reaction with formaldehyde, and can itself be assayed at 425 nm under 275 nm excitation (210).…”

High Pressure Liquid Chromatographic Determination of Pethidine in Plasma

“…Procedures available for the analysis of pethidine in plasma include the colourimetric technique of Burns et al (1955), fluorimetric assay (Dal Cortivo et al, 1970) and the gas chromatographic (GC) methods (Beckett & Tayler, 1967;Goehl & Davison, 1973;Knowles e f al., 1973;Szeto & Inturrisi, 1976). Many of these procedures are associated with problems of one kmd or another.…”

GLC Determination of Meperidine in Blood Plasma

“…This should be true of many other bases as well. Dal Cortivo et al (3) developed a fluorometric assay for meperidine, but a lower limit again of only 0.3 mcg./ml. was established.…”