Fluorometric determination of gentamicin by FIA.

Kubo, Hiroaki; Huang, Yuesheng; Kinoshita, Toshio

doi:10.2116/bunsekikagaku.38.5_215

Cited by 8 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concentration of Free Amino Groups in Various Cheeses as Determined by a Manual Ninhydrin Assay a -reactive amino groups. Studies in this area include (a) photometric analysis of the antibiotic cefalotin with an acid ninhydrin reagent(224), (b) isolation of a toxin from Bordetella pertussis(225), (c) purification of the antibiotic microcin (226), (d) determination of the antibiotics gentamycin and streptomycin by chromatography with a mobile phase containing ninhydrin as a fluorogenic reagent(227)(228)(229), (e) determination of amino glycoside antibiotics(230), (f) characterization of bacereutin, an antifungal antibiotic produced by Bacillus cereus (231), (g) determination of the antibiotic neomycin in ophthalmic ointments (232), (h) analysis of the antibiotic cephalosporin in pharmaceutical samples (233), (i) binding of phosphatidylethanolamine to Actinobacillus pleuropneumoniae(234), (j) analysis of glycine formed during the hydrolysis of hippurate to identify Campylobacter microorganisms (235), (k) analysis of bacterial infection of plant nodules by Rhizobium loti (236a), and the transformation of compost components (236b).…”

mentioning

confidence: 99%

Applications of the Ninhydrin Reaction for Analysis of Amino Acids, Peptides, and Proteins to Agricultural and Biomedical Sciences

Friedman

2004

J. Agric. Food Chem.

519

329

View full text Add to dashboard Cite

The reaction of ninhydrin with primary amino groups to form the purple dye now called Ruhemann's purple (RP) was discovered by Siegfried Ruhemann in 1910. In addition, imines such as pipecolic acid and proline, the guanidino group of arginine, the amide groups of asparagine, the indole ring of tryptophan, the sulfhydryl group of cysteine, amino groups of cytosine and guanine, and cyanide ions also react with ninhydrin to form various chromophores of analytical interest. Since its discovery, extensive efforts have been made to apply manual and automated ninhydrin reactions as well as ninhydrin spray reagents to the detection, isolation, and analysis of numerous compounds of interest across a broad spectrum of disciplines. These include agricultural, biochemical, clinical, environmental, food, forensic, histochemical, microbiological, medical, nutritional, plant, and protein sciences. This reaction is unique among chromogenic reactions in that at pH 5.5 it results in the formation of the same soluble chromophore by all primary amines which react, be they amines, amino acids, peptides, proteins, and even ammonia. Because the chromophore is not chemically bound to the protein or other insoluble material, it is not lost when the insoluble substrate is removed by centrifugation or filtration after the reaction is completed. The visible color of the chromophore is distinctive and is generally not affected by the yellow colors present in many food, plant, and tissue extracts. Adaptations of the classical ninhydrin reaction to specialized needs in analytical chemistry and biochemistry include the use of acid, alkaline, and fluorogenic ninhydrin reagents. To cross-fertilize information among several disciplines wherein an interest in the ninhydrin reaction has developed, and to enhance its utility, this review attempts to integrate and correlate the widely scattered literature on ninhydrin reactions of a variety of structurally different compounds. Specifically covered are the following aspects: historical perspective, chemistry and mechanisms, applications, and research needs. A better understanding of these multifaceted ninhydrin reactions provide a scientific basis for further improvements of this important analytical technique.

show abstract

mentioning

confidence: 99%

Applications of the Ninhydrin Reaction for Analysis of Amino Acids, Peptides, and Proteins to Agricultural and Biomedical Sciences

Friedman

2004

J. Agric. Food Chem.

519

329

View full text Add to dashboard Cite

show abstract

“…1 In the 21st century, however, the discovery rate of novel compounds using traditional screening techniques has been extremely low. Previous methods for the determination of aminoglycosides have included spectroscopy, [2][3][4] chromatography, 5,6 antibiotic activity assay, and immunoassay, 7,8 but new approaches suitable for high-throughput screening will be needed to detect additional aminoglycosides.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Microtiter Plate Assay of Polycationic Aminoglycoside Antibiotics in Culture Broth Using Amaranth

et al. 2017

View full text Add to dashboard Cite

We present a colorimetric method for the detection of aminoglycoside antibiotics such as neomycin (NEO) using a reddish anionic dye, amaranth (AR 3-). Under acidic conditions, at which NEO exists in fully protonated form (NEOH 6+ ), the AR 3-anion associates with the NEOH 6+ cation to form a precipitate, NEOH(AR)2. The precipitate was soluble in a buffer solution of pH 8.5, yielding a reddish solution with an absorption maximum at around 520 nm. Tobramycin and gentamycin, which exist as pentavalent cations under acidic conditions, gave almost the same results. On the other hand, kanamycin, amikacin and streptomycin, which would exist as tri-and tetravalent cations, were not precipitated. Thus, the AR 3-anion could be considered to be an analytical reagent for specific aminoglycosides with polycationic functionality. However, since the precipitation reaction was considerably affected by other anions, a separation method using the tetraphenylborate anion was employed as a pretreatment. The separation method involves precipitating the polycationic aminoglycosides with the tetraphenylborate anion, washing the precipitate with acetonitrile, and re-precipitating the aminoglycosides as hydrochloride salts. Thus, the present method was applied to a microtiter plate assay of the products in an NEO-producing culture broth.

show abstract

“…In the interest of using this kind of antibiotic scientifically and securely, finding a safe range, an optimum dosage and reasonable intervals, and establishing an individualized therapy, it is very important to develop an accurate and quick examination technique for antibiotic serum concentration. Nowadays, the primary methods for the determination of aminoglycoside antibiotics include microbiological assay (MA) [2,3], high-performance liquid chromatography (HPLC) [4,5], ultraviolet visual spectrophotometry (UV-VIS) [6,7], and immune assay (IA) [8,9]. However, MA is too trivial and time consuming to satisfy the need for a quick determination with severely injured and emergency patients, so it is being gradually phased out.…”

Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Hu¹,

Liu²,

Li³

2003

Anal Bioanal Chem

View full text Add to dashboard Cite

In a weakly acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB), and neomycin (NEO), or acid bisazo dye pontamine sky blue (PSB) can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when the two agents react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range 350-600 nm can be observed. The maximum scattering peak is at 580 nm. There is a linear relationship between the RRS intensity and the antibiotic concentration in the range 0.01-6.0 microg mL(-1) at 580 nm. This RRS method has therefore been developed for the determination of trace levels of aminoglycoside antibiotics. The detection limits (3 sigma) of the four antibiotics, whose order of sensitivity is KANA>NEO>TOB>GEN, are 5.8-6.9 ng mL(-1). This method has a good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drops, but clinic serum samples as well. In addition, quantum chemistry-based analysis of the reaction mechanism, the factors influencing the RRS spectra, and the reasons for the enhancement of RRS are discussed.

show abstract

Fluorometric determination of gentamicin by FIA.

Cited by 8 publications

References 0 publications

Applications of the Ninhydrin Reaction for Analysis of Amino Acids, Peptides, and Proteins to Agricultural and Biomedical Sciences

Applications of the Ninhydrin Reaction for Analysis of Amino Acids, Peptides, and Proteins to Agricultural and Biomedical Sciences

Colorimetric Microtiter Plate Assay of Polycationic Aminoglycoside Antibiotics in Culture Broth Using Amaranth

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Contact Info

Product

Resources

About