Fluorometric detection of cancer marker FEN1 based on double-flapped dumbbell DNA nanoprobe functionalized with silver nanoclusters

Li, Bingzhi; Zhang, Peng; Zhou, Bin; Xie, Siying; Xia, Anqi; Suo, Tiying; Feng, Shuang; Xing, Zhou

doi:10.1016/j.aca.2020.12.069

Cited by 22 publications

(14 citation statements)

References 63 publications

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“…Compared with organic dyes and quantum dots (QDs), DNA/AgNCs have the superiority such as longer fluorescence lifetime, higher quantum yield and easy synthesis, low cost, and low toxicity [13,14]. In addition, the formation of AgNCs on the nucleation domain has no significant influence on the other parts of a DNA molecule and the remaining DNA domains still can fold and hybridize [15]. Considering this advantage, DNA/AgNCs can be combined with a variety of different amplification methods to achieve sensitive detection of nucleic acid without labeling.…”

Section: Introductionmentioning

confidence: 99%

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

et al. 2023

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Herein, we developed a novel fluorescent assay using spherical identification probes and toehold-mediated strand displacement reaction-initiated silver nanoclusters (AgNCs) "on-off" signal switch. In this strategy, the target was captured by the spherical probes to induce the activity of exonuclease III (Exo III), catalyzing the cyclic cleavage of substrates to produce a mass of trigger strands. After magnetic bead separation, the intermediates in the supernatant activated downstream toeholdmediated strand displacement reaction to change the structure of silver nanocluster templates, leading to fluorescence intensity reduction. Furthermore, it is demonstrated that the application of spherical identification probes could reduce the signal leakage and the limit of detection. In addition, AgNCs with perfect optical property were ingeniously combined to realize signal output, which reduced the cost and time of synthesis. Under the optimal conditions, the sensing method displayed a good linear range from 250 pM to 25 nM with a detectable minimum concentration of 250 pM. And the practical application potential in complex biological matrices was also evaluated. Considering these advantages, this constructed strategy opens a new path for nucleic acid detection with better performance.

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Section: Introductionmentioning

confidence: 99%

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

et al. 2023

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“…[9][10][11] CRISPR-driven biosensors can achieve high-throughput and field-deployable detection of both nucleic acid [12][13][14] and non-nucleic-acid biomarkers, [15][16][17][18][19] serving as promising tools in food safety, 20,21 environmental monitoring, and medical diagnostics. [22][23][24][25] Currently, innovations of CRISPR-driven biosensors mainly focus on upstream signal processing, while focusing less on the reporting elements. [26][27][28] Most of the previous literature adopts a conventional reporter that a nucleic acid labeled with a pair of fluorophore and quencher (typically referred to as FQ-reporter), which takes a large proportion of the cost of the CRISPR-driven biosensors.…”

Section: Introductionmentioning

confidence: 99%

“…9–11 CRISPR-driven biosensors can achieve high-throughput and field-deployable detection of both nucleic acid 12–14 and non-nucleic-acid biomarkers, 15–19 serving as promising tools in food safety, 20,21 environmental monitoring, and medical diagnostics. 22–25…”

Section: Introductionmentioning

confidence: 99%

A DNA–Cu nanocluster and exonuclease I integrated label-free reporting system for CRISPR/Cas12a-based SARS-CoV-2 detection with minimized background signals

et al. 2022

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“…Traditional assays, including western blot, reverse transcription-polymerase chain reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA) [ 1 , 3 , 4 ] had already been utilized to detect FEN1. Several novel strategies were also designed for FEN1 detection [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. For instance, Zhang et al [ 9 ] proposed a DNA-based fluorescent biosensor to evaluate FEN1 activity in living cells.…”

Section: Introductionmentioning

confidence: 99%

Label-Free and Homogeneous Electrochemical Biosensor for Flap Endonuclease 1 Based on the Target-Triggered Difference in Electrostatic Interaction between Molecular Indicators and Electrode Surface

Zheng

Zhu

et al. 2022

Biosensors

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Target-induced differences in the electrostatic interactions between methylene blue (MB) and indium tin oxide (ITO) electrode surface was firstly employed to develop a homogeneous electrochemical biosensor for flap endonuclease 1 (FEN1) detection. In the absence of FEN1, the positively charged methylene blue (MB) is free in the solution and can diffuse onto the negatively charged ITO electrode surface easily, resulting in an obvious electrochemical signal. Conversely, with the presence of FEN1, a 5′-flap is cleaved from the well-designed flapped dumbbell DNA probe (FDP). The remained DNA fragment forms a closed dumbbell DNA probe to trigger hyperbranched rolling circle amplification (HRCA) reaction, generating plentiful dsDNA sequences. A large amount of MB could be inserted into the produced dsDNA sequences to form MB-dsDNA complexes, which contain a large number of negative charges. Due to the strong electrostatic repulsion between MB-dsDNA complexes and the ITO electrode surface, a significant signal drop occurs. The signal change (ΔCurrent) shows a linear relationship with the logarithm of FEN1 concentration from 0.04 to 80.0 U/L with a low detection limit of 0.003 U/L (S/N = 3). This study provides a label-free and homogeneous electrochemical platform for evaluating FEN1 activity.

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Fluorometric detection of cancer marker FEN1 based on double-flapped dumbbell DNA nanoprobe functionalized with silver nanoclusters

Cited by 22 publications

References 63 publications

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

A DNA–Cu nanocluster and exonuclease I integrated label-free reporting system for CRISPR/Cas12a-based SARS-CoV-2 detection with minimized background signals

Label-Free and Homogeneous Electrochemical Biosensor for Flap Endonuclease 1 Based on the Target-Triggered Difference in Electrostatic Interaction between Molecular Indicators and Electrode Surface

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