Fluorine-containing block/branched polyamphiphiles forming bioinspired complexes with biopolymers

Paiuk, Olena; Mitina, Nataliya; Šlouf, Miroslav; Pavlová, Ewa; Finiuk, Nataliya; Kinash, Nataliya; Karkhut, Andriy; Manko, Nazar; Gromovoy, Taras Yu.; Hevus, Orest; Shermolovich, Yu. G.; Stoika, Rostyslav; Zaichenko, Alexander

doi:10.1016/j.colsurfb.2018.11.047

Cited by 18 publications

(10 citation statements)

References 25 publications

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“…Subsequently, the grid was removed from the oven and then the excess solvent on the grid was removed by touching the bottom of the grid with a small piece of filter paper. This fast removal of the solution is a key step of the fast-drying method, which minimizes drying artifacts, as shown in our previous studies. − The dried samples were equilibrated for at least 1 h in air and were then observed under TEM at room temperature using bright field imaging at 120 kV.…”

Section: Methodsmentioning

confidence: 99%

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics

et al. 2021

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Fluorine-19 magnetic resonance imaging (19F MRI) enables detailed in vivo tracking of fluorine-containing tracers and is therefore becoming a particularly useful tool in noninvasive medical imaging. In previous studies, we introduced biocompatible polymers based on the hydrophilic monomer N-(2-hydroxypropyl)methacrylamide (HPMA) and the thermoresponsive monomer N-(2,2-difluoroethyl)acrylamide (DFEA). These polymers have abundant magnetically equivalent fluorine atoms and advantageous properties as 19F MRI tracers. Furthermore, in this pilot study, we modified these polymers by introducing a redox-responsive monomer. As a result, our polymers changed their physicochemical properties once exposed to an oxidative environment. Reactive oxygen species (ROS)-responsive polymers were prepared by incorporating small amounts (0.9–4.5 mol %) of the N-[2-(ferrocenylcarboxamido)ethyl]acrylamide (FcCEA) monomer, which is hydrophobic and diamagnetic in the reduced electroneutral (Fe(II), ferrocene) state but hydrophilic and paramagnetic in the oxidized (Fe(III), ferrocenium cation) state. This property can be useful for theranostic purposes (therapy and diagnostic purposes), especially, in terms of ROS-responsive drug-delivery systems. In the reduced state, these nanoparticles remain self-assembled with the encapsulated drug but release the drug upon oxidation in ROS-rich tumors or inflamed tissues.

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Section: Methodsmentioning

confidence: 99%

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics

et al. 2021

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“…Azoisobutyronitrile (AIBN), poly(ethylene glycol), Mn = 550 (PEG), ammonium cerium (IV) nitrate were received from Aldrich Chemical and used without purification. Peroxy-containing chain transfer agent 1-isopropyl-3(4)-[1-(tert-butylperoxy)-1-methylethyl]benzene (MP) was synthesized as described earlier [8].…”

Section: Methodsmentioning

confidence: 99%

An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration

et al. 2021

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Antisense-DNA technologies are new strategy for the treatment of prion infections. This strate gy requires prolonged administrations of the drugs, which are likely to alter cell redox processes. Aim. The evaluation of cell survival and intensity of oxidative processes in vitro under the influence of antisense-oligodeoxynucleotides (asODNs) as cell prion inhibitors (PrP C ) complexed with cationic polyelectrolyte. Methods. Free diffusion in agarose gel, study of cytotoxic action on model cells (bull semen), polarography and potentiometric measurement of oxygen uptake, statistical analysis. Results. Poly(dimethylaminoethyl methacrylate) [-]based surfactants form complexes with asODNs. Polyethylene glycol containing surfactants increase oxygen uptake by cells: by 18 % (VI), by 37 % (IV) and 2.6-fold for V. An addition of the IV-asODNs complex into [the] cell medium did not affect the oxygen absorption; however, it increased reduction processes. Interpolyelectrolyte complex V-asODNs increased the cell respiration by 1.95 times. VI separately increased the cell absorption of oxygen by 18 % and in the complex with asODNs -by 36 %. VI possessed the lowest cytotoxicity. Conclusions. New cationic polyelectrolytes form complexes with asODNs. VI causes the smallest effect on the RedOx processes of model cells and possesses the lowest cytotoxic effect. K e y w o r d s: antisense oligodeoxynucleotides, polyelectrolyte complexes, cytotoxicity, redox processes, prion.

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“…This fast solution removal, which takes less than 1 s, minimizes drying artifacts as evidenced in our previous studies. 32,33 The dried samples were left to equilibrate at room temperature for 1 h and then they were observed in the TEM microscope using a bright field imaging at 120 kV.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Anionically-functionalized glycogen encapsulates melittin by multivalent interactions

Zhukouskaya

Blanco

Černochová

et al. 2022

Preprint

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We developed acid-functionalized glycogen conjugates as supramolecular carriers for efficient encapsulation and inhibition of a model cationic peptide melittin - the main component of honeybee venom. For this purpose, we synthesized and characterized a set of glycogens, functionalized to various degrees by several different acid groups. These conjugates encapsulate melittin up to a certain threshold amount, beyond which they precipitate. Computer simulations showed that sufficiently functionalized conjugates electrostatically attract melittin, resulting in its efficient encapsulation in a broad pH range around the physiological pH. Hemolytic assays confirmed in vitro that the effective inhibition of melittin’s hemolytic activity occurs for highly functionalized samples, whereas no inhibition is observed when using low-functionalized conjugates. It can be concluded that functional glycogens are promising carriers for cationic molecular cargos or antidotes against animal venoms under conditions, in which suitable properties such as biodegradability and biocompatibility are crucial.

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Fluorine-containing block/branched polyamphiphiles forming bioinspired complexes with biopolymers

Cited by 18 publications

References 25 publications

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics

An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration

Anionically-functionalized glycogen encapsulates melittin by multivalent interactions

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Fluorine-containing block/branched polyamphiphiles forming bioinspired complexes with biopolymers

Cited by 18 publications

References 25 publications

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for 19F MRI Theranostics

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for 19F MRI Theranostics

An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration

Anionically-functionalized glycogen encapsulates melittin by multivalent interactions

Contact Info

Product

Resources

About

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics

Thermo- and ROS-Responsive Self-Assembled Polymer Nanoparticle Tracers for ¹⁹F MRI Theranostics