An influence of complexes of therapeutic antisense oligodeoxynucleotides with cationic polymers on cell respiration

Abstract

Antisense-DNA technologies are new strategy for the treatment of prion infections. This strate gy requires prolonged administrations of the drugs, which are likely to alter cell redox processes. Aim. The evaluation of cell survival and intensity of oxidative processes in vitro under the influence of antisense-oligodeoxynucleotides (asODNs) as cell prion inhibitors (PrP C ) complexed with cationic polyelectrolyte. Methods. Free diffusion in agarose gel, study of cytotoxic action on model cells (bull semen), polarography and potentiometric measurement of oxygen uptake, statistical analysis. Results. Poly(dimethylaminoethyl methacrylate) [-]based surfactants form complexes with asODNs. Polyethylene glycol containing surfactants increase oxygen uptake by cells: by 18 % (VI), by 37 % (IV) and 2.6-fold for V. An addition of the IV-asODNs complex into [the] cell medium did not affect the oxygen absorption; however, it increased reduction processes. Interpolyelectrolyte complex V-asODNs increased the cell respiration by 1.95 times. VI separately increased the cell absorption of oxygen by 18 % and in the complex with asODNs -by 36 %. VI possessed the lowest cytotoxicity. Conclusions. New cationic polyelectrolytes form complexes with asODNs. VI causes the smallest effect on the RedOx processes of model cells and possesses the lowest cytotoxic effect. K e y w o r d s: antisense oligodeoxynucleotides, polyelectrolyte complexes, cytotoxicity, redox processes, prion.

Bioenergetic characteristics of the murine Nemeth-Kellner lymphoma cells exposed to thiazole derivative in complex with polymeric nanoparticles

“…The complexes of the investigated thiazole derivative with PEG containing polymer nanoparticles (PEGPNs) were formed to improve the solubility of BF1 and increase its penetration. It was shown, that influence of poly ethylene glycolcontaining surfactants changed cell respiration in different types of diseases [2,20]. It was previously established, that the studied com plexes were more cytotoxic to certain tumor cell lines compared to the control than unconjugated BF1 [21].…”